Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 40 for “"Cell surface markers"”.
-
An unbiased proteomic approach to identifying cell surface markers in neutrophils
… Efferocytosis refers to the engulfment of dead cells by phagocytes such as neutrophils and macrophages and culminates in the beneficial removal of inflammatory cargo and auto-reactive material. While many of the mechanisms involved in efferocytosis have been described, the critically important …
-
Defining the Transcriptional and Epigenetic Signature of Mouse Embryonic Stem Cells with Compromised Developmental Potency
Mouse embryonic stem (ES) cells have played a crucial role in studying developmental processes and gene function in vivo. They are extremely useful in the generation of transgenic animals as they can be genetically manipulated and subsequently microinjected into blastocyst stage embryos, where they …
-
Immune Function Determination in Mice Dermally Exposed to Permethrin
… macrophage phagocytic activity was not affected. Cell surface markers of thymocytes, splenocytes and bone marrow cells were not affected. Antibody production as shown by plaque forming cell (PFC) assay decreased significantly at 10 days after dosing termination. Taken together, these data indicate …
-
Characterisation of the T cell responses induced by BCG in infants over the first year of life
… the first study was to characterise specific T cell immunity following BCG vaccination. These data are critical to determine when to optimally boost BCG induced immunity in infants. We enrolled infants routinely vaccinated with BCG at birth, and determined the frequency of T cells induced by …
-
Identification and characterisation of HLA-DRB1*0101 restricted CD4+ T cell epitopes in the major grass pollen allergens Phl p 1 and Phl p 5
… thesis was to identify HLA-DR1-restricted CD4+ T cell epitopes of Phl p 1 and Phl p 5 by complementary approaches of epitope mapping: in silico prediction, tetramer guided epitope mapping and human HLA-DR1 transgenics. I investigated recognition of HLA-DR1-restricted CD4+ T cell epitopes in …
-
Microfluidic devices for analysis of red blood cell mechanical properties
Decreased deformability of human red blood cells (RBCs) is both a cause of disease and biomarker for disease (1). To traverse blood capillaries, the biconcave disk-shaped RBC must deform dramatically, since the diameter of the unconstrained RBC is larger than that of the capillaries. If the RBC …
-
Mechanism of TCDD-Induced Immunotoxicity: The Role of Cell Activation in the Generation of Toxicity
… that TCDD exerted immunotoxic effects only on cells differentiating in response to antigenic challenge. To this date, no work has been done to characterize the long-term effects of TCDD on the activated cells. Additionally, no studies have been done to determine whether TCDD has any effect on …
-
The development of an in vivo model to study the biology and treatment of childhood acute lymphoblastic leukaemia (ALL)
… expansion and characterisation of childhood ALL cells, obtained from patients at diagnosis or relapse. Mice were inoculated with leukaemia cells from patients' biopsies and engraftment was monitored by the proportion of human CD45+ cells in the blood. Successful leukaemia engraftment was achieved …
-
Characterization of porcine adipose tissue-derived stem cells
… suggests the use of a less differentiated donor cell, such as a stem cell, may lead to an increase in the efficiency of the NT procedure. Stem cell characteristics must be clearly defined in order to isolate, identify and optimally culture potential donor cells. Stem cell characteristics such as …
-
The role of T cell Immunoglobulin Mucin domain in Herpes Simplex Virus-induced Behçet’s Disease mouse model
The T cell immunoglobulin mucin (TIM) proteins regulate T cell activation and tolerance. Individual TIM family members may serve as susceptibility markers for asthma, allergies and autoimmune diseases, as well as potential cell surface markers for T helper type (Th)1 and Th2 T cells. TIM-1 plays an …
-
Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… Our lab uses human induced pluripotent stem cells (iPSCs), as an attractive alternative supply, as iPSCs can be cultured indefinitely and differentiate into almost any cell type. Using a technique called forward programming, we over express three key haematological transcription factors …
-
Investigation of neuron T cell interaction in central nervous system tuberculosis
… understanding of the CNS immunity; investigate cells targeted for infection, their respective response to infection and interaction with different cell types to the overall protection of the CNS - as accumulating evidence indicates a dynamic neuronal lymphocyte interplay that defines outcomes of …
-
Development of one-step single-cell RT-PCR for the massively parallel detection of gene expression
… the presence of a reservoir of latently infected cells, such as resting CD4+ T-cells. The latent reservoir in resting memory CD4+ T-cells has been estimated to decay with a half-life of as long as 44 months, thus hindering the eradication of HIV. Current knowledge of latent reservoirs came from …
-
The pivotal role of dendritic cells in feline immunodeficiency virus infection
… to human immunodeficiency virus. Dendritic cells (DC) are one of the first cell types encountered during lentivirus infections. The studies set forth in this dissertation initiated investigations into the interactions of FIV and DC. Accordingly, objectives addressed here were: (1) to develop …
-
REGULATION OF TELOMERASE EXPRESSION IN STEM CELL REPROGRAMMING
… easily accessible source of patient-specific cells that will function for use in regenerative medicine. One promising source is the adult stem cell derived from adipose tissue (ASCs). Isolated from waste lipoaspiration, these cells could serve as a readily available source for the regeneration …
-
Characterization and application of adipose derived stem cells following burn injury
… that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we investigated the cellular source of inflammation within adipose tissue and whether inflammation affects ASC fate and …
-
In Vivo Studies on the Transcriptional and Posttranslational Regulation of the CCAAT/Enhancer Binding Protein Beta
… macrophage population, showed that the cells are Mac1(+), F4/80(+) and Gr1(+), typical markers of Natural Suppressor macrophages. Taken together, the C/EBPbeta target genes found in the macrophage and the cell surface markers, suggest an immunosuppressive phenotype. we propose a novel …
-
A comparison of gene expression in tolerated and rejected islet grafts by gene microarray analysis
… of tolerance and good graft function mononuclear cell infiltration surrounding the islet was found in tolerated grafts. To elucidate the differences between these mononuclear infiltrates in the presence of tolerance and destructive mononuclear infiltrates during graft rejection as well as their …
-
CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
Human pluripotent stem cells (hPSCs) exist in multiple states of pluripotency, broadly categorised as naïve and primed states. These provide an important model to investigate the earliest stages of human embryonic development. Naïve cells can be obtained through primed-to-naïve reprogramming; …
-
Establishing models of and characterising the genomic context of neutrophil hypersegmentation
… contexts, suggesting it may not be a marker of cell maturity, but rather one of functional status. Neutrophil hypersegmentation has been described in response to a wide array of stimuli, and clinically in the context of tumour-associated neutrophils (TANs), bacterial infection and in the lung …
Page 1 of 2