Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 164 for “"Cell sorting"”.
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Affinity Flow Fractionation for label-free cell sorting
Capture and isolation of flowing cells from body fluids such as peripheral blood, bone marrow or pleural effusion has enormous implications in diagnosis, disease monitoring, and drug testing. However, in many situations the conventional methods of cell sorting are of limited use due to complex …
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Surface Acoustic Wave-Actuated Cell Sorting and Enantiomer Separation
… modeling and numerical simulations of cell sorting and enatiomer separation using surface acoustic wave-actuated fluid flow. We model a high throughput sorting of two different types of biological cells (type I and type II) by a biomedical micro-electro-mechanical system (BioMEMS) whose …
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Enabling technologies for multiplexed biomolecule analysis and cell sorting
… research. From a diagnostics standpoint, the cells, proteins, and nucleic acids that compose our bodies contain an enormous amount of information that can indicate the presence of, progression of, or even susceptibility to a given disease. However, extracting this information is often quite …
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Inexpensive technologies enabling widespread utilization of image-predicated cell sorting
The most mature, widespread sorting technology, fluorescence-activated cell sorting (FACS), offers high throughput and sorts predicated on a wide range of phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of …
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
… are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a …
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Contactless Dielectrophoresis towards Drug Screening and Microdevice Development for Cell Sorting
… phenotype of late-stage ovarian cancer cells to a profile similar to that of slow-growing early-stage ovarian epithelial cells after treatment with a non-toxic bioactive metabolite, sphingosine. Current chemotherapeutics are highly toxic to patients and can cause severe adverse side …
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Tools for engineering multicellular systems through cell sorting and cell state detection
… human genome, the genetic blueprint that every cell in our body follows, encodes approximately 20,000 genes. Through complex regulation of these genes, each cell is able to play the role it needs within our body. Synthetic biology, an emerging field in biology, seeks to expand on this blueprint …
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Design, Fabrication and Characterization of a Microfluidic Device for Ultrasound-based Cell Sorting
The isolation and sorting of cells is an important process in research and hospital laboratories. Most research laboratories incorporate fluorescently or magnetically labeled antibodies adherent to cell surface antigens for cell identification and separation. MiCS (Microbubble Cell Sorting) is a …
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Propulsion of polymer particles on caesium ion-exchanged channel waveguides for stem cell sorting applications
… of a channel waveguide potentially allows for sorting and trapping of several particles and cells simultaneously. In the evanescent field above an optical channel waveguide, particles experience three optical forces; i) a transverse gradient force, which acts in the direction of the intensity …
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A two-color bacterial two-hybrid system for selecting sequence-specific DNA-binding proteins via fluorescence activated cell sorting
Proteins that recognize specific DNA sequences play a major role in many biological processes, and the ability to select or design novel DNA binding proteins could have a major impact on many areas of biotechnology and medicine. This thesis begins with background information on zinc finger …
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Mechanotransduction at cell-cell contacts
… whether the differences in cadherin- dependent cell-cell adhesion energies could solely predict cell sorting. Although adhesion energies do influence cell sorting in vitro, recent studies suggested that that differences in cell adhesion, determined mainly by cadherin binding affinities and …
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Identification of echinus and characterization of its role in Drosophila eye development
… eye results from a coordinated process of cell sorting, differentiation and selective cell death in the retinal epithelium. Mutations in the gene echinus cause supernumerary pigment cells due to insufficient cell death. This study reports the identification of echinus and the …
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Microfluidic Concentration Gradient Generation and Integrated Magnetic Sorting of Microparticles
… their feature size similar to that of biological cells, have great potential for cell manipulation and interrogation. On the other hand, the process of drug discovery involves vast amount of tests of candidate drug molecules with cells, and hence requires intensive manipulation and interrogation …
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… such as cancer, use by pathogens to infect host cells, and roles in the immune system makes them important targets for the design of therapeutics and diagnostics. However, a major limitation in targeting the vast array of glycans has been the lack of reliable reagents. Many existing …
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An integrated system for selection and manipulation of single cells in culture
Cellular heterogeneity plays a critical role in processes like embryonic development, drug-resistance, and immune response. The ability to select and manipulate individual cells greatly increases the ability to study heterogeneity within cultures. As such, a number of different single cell sorting …
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Cadherin specificity in adhesion and mechanotransduction
Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. Tight regulation of cadherin adhesion plays critical roles in diverse biological processes. Differential binding between cadherin subtypes is widely believed to mediate cell sorting during embryogenesis. However, a …
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Homophilic and Heterophilic Interactions Among Cadherin Cell Adhesion Molecules
… over heterophilic binding that would explain the cell sorting behavior.
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Cell compliance: cytoskeletal origin and importance for cellular function.
Mechanical properties of cells, mainly defined by their cytoskeleton, are closely related to cell function and can be measured with a dual-beam laser trap (optical stretcher). Functional changes, which go hand in hand with changes of the cytoskeleton, also occur during differentiation of stem …
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Lab on a chip rare cell isolation platform with dielectrophoretic smart sample focusing, automated whole cell tracking analysis script, and a bioinspired on-chip electroactive polymer micropump
… Contactless DEP (cDEP) is a recently developed cell sorting and isolation technique that uses the DEP force by capacitavely coupling the electrodes across the channel. The cDEP platform sorts cells based on intrinsic biophysical properties, is inexpensive, maintains a sterile environment by …
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Regulation of Homophilic and Heterophilic Adhesion Among Classical Cadherins
… and actin organization in E-cadherin expressing cells cultured on E-, N-, and C-cadherin ectodomains. As expected, the formation of filamentous actin correlated with the Rac activation amplitudes in E-cadherin expressing cells. The results also showed that lateral cadherin clustering, which is …
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