Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Cell reprogramming"”.
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Modularity in Somatic Cell Reprogramming
Reprogramming relies on the concept that cellular fate is plastic, and therefore a considerable effort has been made to develop molecular strategies that reset the epigenome to achieve distinct cell fates, including stem cells. Deriving induced pluripotent stem (iPS) cells has thus emerged as a …
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Novel Regulators of Somatic Cell Reprogramming
Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by expression of defined embryonic factors. My thesis is focused on exploring the mechanisms underlying reprogramming using a secondary mouse embryonic fibroblast model that forms iPS cells with high efficiency upon inducible …
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Modelling Huntington’s Disease Using Direct Cell Reprogramming
… of generating a representative model of HD. Cell reprogramming is a revolutionary technology that can generate live, human neurons in vitro from the somatic cells of individuals with HD. Our research group was the first to demonstrate the direct reprogramming of adult human dermal fibroblasts …
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REGULATION OF TELOMERASE EXPRESSION IN STEM CELL REPROGRAMMING
… easily accessible source of patient-specific cells that will function for use in regenerative medicine. One promising source is the adult stem cell derived from adipose tissue (ASCs). Isolated from waste lipoaspiration, these cells could serve as a readily available source for the regeneration …
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Chromatin Regulation in Somatic Cell Reprogramming to Pluripotency
Contains fulltext : 215040.pdf (Publisher’s version ) (Open Access)
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Computational Systems Biology Analysis of Cell Reprogramming and Activation Dynamics
In the past two decades, molecular cell biology has transitioned from a traditional descriptive science into a quantitative science that systematically measures cellular dynamics on different levels of genome, transcriptome and proteome. Along with this transition emerges the interdisciplinary …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
Human pluripotent stem cells (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic …
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The Modeling Spectrum of Data-Driven Decision Making
… spectrum in the contexts of digital agriculture, cell reprogramming, and pandemic policymaking. First, we investigate the problem of learning good farming practices in the framework of multi-armed bandits with expert advice. We extend the setting from finitely many experts to any countably …
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Reprogramming to Pluripotency Using Small Molecule Compounds
<p>The generation of induced pluripotent stem cells (iPSCs) through the use of small molecule compounds has evolved as a potential cellular reprogramming strategy. Individually, specific small molecule compounds have previously been shown to replace reprogramming transcription factors or enhance …
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Induced pluripotency and reprogramming by defined factors
… genome is the foundation for epigenetics and cellular differentiation. Somatic cell nuclear transfer experiments (SCNT) provided unequivocal proof of nuclear equivalency between adult somatic cells. However the molecular mechanisms of somatic cell reprogramming have remained largely a mystery. …
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Modeling Alzheimer's Disease Using Cellular Reprogramming Technologies
Two cellular reprogramming technologies have emerged that demonstrate that cell-fate can be converted by ectopic expression of defined transcription factors: induced pluripotent stem (iPS) cell technology and induced neuronal (iN) cell technology. These recent advances in cell reprogramming …
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Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response
Ehrlichia chaffeensis is an obligately intracellular bacterium that establishes infection in mononuclear phagocytes through largely undefined reprogramming strategies including modulation of host gene transcription. In this study, we demonstrate that the E. chaffeensis effector TRP47 enters the …
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Visualizing the Structural Basis of Genome Silencing
… reflects both the transcription profile of the cell and of the specific cell type. A dispersed, mesh-like chromatin configuration, for example, is characteristic of a pluripotent stem cell. Here we show that the acquisition of the pluripotent state during somatic cell reprogramming is coincident …
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Distinct Functional Properties of Perinatal Versus Adult Adipose Progenitor Subpopulations
… metabolic syndrome. The recruitment of new fat cells ensures adequate energy storage in WAT and prevents against ectopic lipid accumulation and is therefore considered as "healthy expansion". Pathologic WAT expansion is characterized by chronic tissue inflammation and fibrosis, and is tightly …
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Molecular mechanisms of pioneer factor ELF2 interactions with the nucleosome
… regions. Pioneer factors are important for stem cell pluripotency, cell reprogramming and differentiation. ETS family TFs are a large subfamily of TFs consisting of 29 members. The DNA binding domains (DBD) of ETS family TFs are highly conserved. ELF2 is a ETS family pioneer factor that has a …
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Establishment of the epigenetic landscape in mammalian embryonic stem cells
… phenomena has the potential to enrich both stem cell biology and therapeutics, as well as offer insight into various pathologies. While the enzymatic machinery responsible for these transitions is well known, it is their localization to specific genomic regions that controls cell fate, and this …
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Chromatin accessibility informs cell identity: studies in silico, in vitro, and in vivo
… provides key regulation in defining cell identity as it exhibits control over the ability of transcription factors and transcriptional machinery to bind to regulatory elements and initiate changes to gene expression. In this thesis I elaborate on the intricate relationships between …
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