Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Cell permeability"”.
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
Bacterial cell genetic engineering is fundamental for research aiming to learn more about bacterial species for a broad range of applications. One method of intracellular delivery of foreign DNA during the genetic engineering process is the use of electroporation to create pores along the bacterial …
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Investigations into endothelial cell permeability and adherens junction disruption induced by Junín virus infection.
… to produce productive infection of endothelial cells in vitro with no visible cytopathic effects which provides a unique opportunity to study the cells while they are infected. Here, we show that direct Junín virus infection of primary human endothelial cells (EC) corresponds to increased …
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Synthesis of aromatic peptide nucleic acids
… to a more hydrophobic backbone which may improve cell permeability. Such analogues may be useful as antisense/antigene therapeutic agents. A first generation APNA hexamer, composed of monomers of general structure A was synthesized in solution. Thermal denaturation studies failed to indicate if …
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An evaluation of the theory and use of the acridine orange stain in enumerating bacterial populations
… reports in the literature. Green fluorescence of cells is not a reliable indicator of viability; and fluorescence color alone cannot distinguish between detritus and bacteria. Instead, the resultant color of fluorescence, i.e., green or red, appeared to relate to AO concentration and cell …
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Endothelial cells and basement membrane : a co-regulatory unit for fibroblast growth factor-2 in hyperglycemic stress
Endothelial cells and basement membrane interact as a biochemical and mechanical co-regulatory unit. The wide spectrum of manifestations of diabetic vascular disease could be related to altered kinetics of vasoactive compounds within this regulatory unit. We hypothesized that hyperglycemic stress …
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Design and synthesis of novel functionalized sensors for the biological imaging of zinc(II) and nitric oxide
… used to image chelatable zinc(II) in neuronal cells. Ratiometric sensors have been developed for more precise zinc sensing. These sensors exhibit differentiable changes in fluorescence in the presence and absence of zinc. CHAPTER 2. An Esterase-Activated System for the Ratiometric Sensing of …
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Cell penetrating peptide for ocular drug delivery
… and the vasculature of the eye, leading to cell death, vascular permeability and vision loss. Cx43 HC block has proven to be effective in inhibiting the inflammatory cascade in various in vitro and in vivo ocular models. Gap19 is an intracellularly acting connexin mimetic peptide, said to be …
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The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
… genes at this point in the central dogma of cell biology offers the chance for excellent gene knockdown specificity as the gene silencing tool is tailored for an exact messenger RNA sequence. There are some inherent problems associated with this type of technology which arise from the …
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Chemical Tools to Monitor and Inhibit poly(ADP -Ribosyl)ation
… of PARG, due to toxicity (intercalators), cell permeability issues (ADP-HPD), and nonspecific activity (tannins), few if any of these compounds have been useful for evaluating the effects of PARG inhibition in cell culture and in vivo. Thus, efforts to synthesize and assess cell ADP-HPD …
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The synthesis of bimane constrained peptides and their fluorescent and structural properties
… small molecule drug hurdles including cell permeability, protease degradation and the ability to visualise and track a molecule intracellularly. Linkers such as the all-hydrocarbon metathesis linker have shown great promise in reducing protease degradation and increasing cell …
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Investigation of zinc metalloneurochemistry with fluorescent sensors based on fluorescein platforms
… Platform: Synthesis, Properties and Intracellular Distribution Two new fluorescent sensors for Zn2' that utilize fluorescein as a reporting group, Zinpyr-1 (ZP1) and Zinpyr-2 (ZP2), have been synthesized and characterized. ZP1 is prepared in one step via a Mannich reaction, and ZP2 is …
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Investigation of catalase and superoxide dismutase from Mycobacterium avium, M. intracellulare and M. scrofulaceum
… but not peroxidase activity was detected in cell-free extracts of Mycobacterium avium, M. intracellulare and M. scrofulaceum (MAIS). The M. scrofulaceum isolates had the highest catalase activity, while both M. avium and M. intracellulare had significantly lower activities. The percentage of …
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Great Expectations: Phosph(On)Ate Prodrugs In Drug Design—Opportunities and Limitations
… physiological pH, which portends poor membrane permeability. This limitation has been successfully overcome through the use of prodrugs. When attached to the phosph(on)ate moiety, prodrugs mask the negative charge and easily enable cell permeability. Upon cellular entry, the promoieties are …
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The Development of Novel Tools for the Metabolic Labelling of Glycans in Cancer
Aberrant cell surface glycosylation is key in tumour proliferation, metastasis, and immune evasion, with hypersialylation one of the alterations most observed in tumour cells. Changes in the glycome can be monitored by incorporating unnatural sugars bearing bioorthogonal chemical reporters. …
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Targeting PfUCHL3 Using Chemically Constrained Peptides
… disease. PfUCHL3 is essential to *P. falciparum* cell viability, and through the use of the RaPID peptide discovery system (Yamagishi Y et al, 2011), 10 novel, non-natural, cyclic peptides were selected for against PfUCHL3. The peptides all display tight dissociation constants for PfUCHL3 in the …
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Controlling Gene Expression with Synthetic Molecules
… and other biomolecules generally have poor cell-membrane permeability and are prone to rapid enzymatic inactivation inside cells, it is highly desirable to develop artificial molecules that are capable of mimicking native ADs. Towards this goal, a comprehensive methodology for the synthesis, …
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Understanding the intrinsic antibiotic resistance in Lysobacter enzymogenes, a ubiquitous environmental bacterium
… characterization of selected mutants showed that cell permeability, β-lactamase activity and transport contributed to high level of resistance to ampicillin in LeC3; whereas high level of resistance to spectinomycin in LeC3 was mostly due to its ribosomal RNA target as well as related to cell-wall …
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Synthesis of Novel Sulfonamide-Based Calpain Inhibitors and Their Potential as Anti-Tumor Agents
<p>Calpain is a class of intracellular cytoplasmic cysteine proteases.1 The enzyme participates in different intracellular signaling pathways that are mediated by Ca2+.2 The two major isoforms of calpain universally distributed in most mammalian tissues are calpain 1 (µ-calpain) and calpain 2 …
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Design, Synthesis, and Antibacterial Properties of Dual-Ligand Inhibitors of Acetyl-CoA Carboxylase and Mechanism of Action for the Antibacterial Agent Moiramide B
… activities, likely reflects alteration in cell permeability or avoidance of intrinsic efflux of the tested dual-ligand molecules. The natural product moiramide B is a broad spectrum antibiotic that inhibits the carboxyltransferase component of acetyl-CoA carboxylase. A crystal structure of …
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