Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Cell penetrating peptide"”.
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Cell penetrating peptide for ocular drug delivery
… and the vasculature of the eye, leading to cell death, vascular permeability and vision loss. Cx43 HC block has proven to be effective in inhibiting the inflammatory cascade in various in vitro and in vivo ocular models. Gap19 is an intracellularly acting connexin mimetic peptide, said to be …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
… charge of siRNA creates a hurdle for intracellular delivery that has thus far limited its development as a therapeutic. Cell penetrating peptides (CPPs), such as the Antennapedia homeodomain (AntpHD) and its third helix, Penetratin, are well characterised cationic motifs used previously to …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
<p>Somatic stem cells have been used as a regenerative medicine tool to treat diseases like leukemia for decades, but they are limited in their ability to self-renew and differentiate. A better alternative, induced pluripotent stem cells (iPSCs), has the ability to generate cells from all three …
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Delivering Signal-Altering Bacterial Effector Proteins to Mammalian Cells Using Cell-Penetrating Peptide Technology
… inflammation, the transcription of antimicrobial peptides, the modulation of the cell cycle and cell survival. Some Gram-negative bacteria have evolved needle-like structures called Type III Secretion Systems (T3SS) that secrete signal-altering molecules into the host cell to interrupt signaling …
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Simultaneous Delivery of CRISPR/Cas Complexes and Donor DNA Using Cell-Penetrating Peptide-Adaptors
… with RNA to the nuclei of living eukaryotic cells using a novel cell-penetrating peptide (CPP)-adaptor system. Since the discovery of CPPs in the 1980s, researchers have used CPPs to deliver biomolecules such as proteins and nucleic acids into cells (the delivered biomolecules are called …
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
<p>Induced pluripotent stem cells (iPSCs) are one of the most promising new technologies in the field of regenerative medicine. iPSCs can be generated directly from a patient’s own fibroblast cells then converted into any cell type, thus overcoming challenges associated with embryonic stem cells …
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Utilization of the Cell Penetrating Peptide TAT-CaM in New Biological Systems: Fungal and Bacterial Cell Lines
<p>Cell penetrating peptides (CPPs) are biomolecular vehicles or systems that mediate the delivery of proteins into different cell lines. Since their discovery in the late 1980s, the CPP delivery system has primarily been explored in different types of mammalian cells. The technology has …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
… of HIV through the plasma membrane of mammalian cells. The McMurry lab developed a CPP-adaptor system which consists of the fusion of TAT to calmodulin to which mediates the delivery of exogenous proteins into cells and prevents endosomal entrapment. Calmodulin (CaM) is capable of binding to the …
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Development of a Real-Time Cell Imaging Assay to Characterize the Delivery of a Cell-Penetrating Peptide Adaptor and its Associated Cargo
<p>Cell-penetrating peptides (CPPs) are small peptides capable of transporting molecules across the membrane. In most cases, the membrane is a blockade that prevents molecules from entering the cell, therefore CPPs have attracted much attention. Although CPPs are effective, this system is still …
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Targeting Nrf2 in Inflammation and Cancer
The transcription factor Nrf2 protects against cellular stress by inducing cytoprotective proteins. Activation of Nrf2 protects against inflammation and oxidative damage in disease models in vitro and in vivo. Nrf2 activation may be a good therapeutic strategy in these diseases. Some dietary …
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A Dual-Domain Peptide Strategy for Targeted Regenerative Repair
… aims to develop and evaluate a modular synthetic peptide designed to enhance tissue regeneration through coordinated extracellular matrix targeting and immune modulation. The peptide construct incorporates a collagen-binding domain (CBD) to spatially localize bioactivity within matrix-rich …
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Enhancing the radio sensitivity of prostate cancer using RALA Gold nano-complexes
… systemic circulation, poor stability and target cell uptake. This thesis employs the cell penetrating peptide RALA to enhance AuNP cell internalisation and in turn radiosensitivity of PCa. RALA-AuNP have undergone extensive characterisation following synthesis, proving optimal for in vitro and in …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… & translation, protein degradation and cell cycle progression. Furthermore, evidence suggest that misregulation of O-GlcNAcylation may result in pathogenesis, such as Alzheimer’s and diabetes. To elucidate the function of O-GlcNAc it is crucial to understand the regulation of OGT and OGA …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… of nucleic acid therapeutics across various cell membranes has always been one of the major challenges. Cell-penetrating peptides (CPPs) containing arginine (R), tryptophan (W), and histidine (H) have been promising for siRNA delivery. Therefore, to enhance siRNA delivery and efficient …
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Acetylation regulates Thioredoxin Reductase activity and oligomerization
The Thioredoxin (Trx) system provides the cell with robust defense against oxidative stress and regulates the function of nearly every cellular process through the reduction-oxidation (redox) regulation of proteins. The Trx system is involved in the development of many diseases ranging from cancer …
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Mitochondrial Unfolded Protein Response Regulator Atf5 In Mitochondrial Targeted Therapies In Aml
… protein pool homeostasis. However, the cell-protective roles of UPR<sup>mt</sup> in the context of mitochondrial stress-induced cell death in AML has not been well explored. We demonstrate that AML cells are susceptible to mitochondrial targeted agents such as ONC201, an agonist of the …
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I) Hv1, LBP and LCF reconstitution in Liposomes II) Signed, Sealed, and Delivered: CPP Technology Delivers Active Protein Cargo
… Hv1. The second half of this thesis focuses on cell penetrating peptide (CPP) technology. TaT-CaM is a recently developed CPP that crosses cellular membranes and carries a wide variety of proteins. This study focuses on post-delivery effects of catalase, a peroxidase protein that breaks down …
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Design and Sythesis of Conjugages of Amphiphilic Cell-Penetrating Peptides Containing Anticancer Drug and Ligand for Extra Cellular Matrix Biomarker to Provide Efficient Tumor-Targeting
<p>Peptide-drug conjugates (PDCs) have emerging applications in the safer delivery of chemotherapeutics to the site of diseases to avoid the side effects associated with the drug. PDCs also improve the physicochemical property of the anticancer drug. Therefore, we have designed and synthesized …
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