Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"Cell mechanics"”.
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Effect of Cell-Substrate Interactions on Epithelial Cell Mechanics
Cell-substrate interactions play a key role in the regulation of epithelial cell mechanics. Through a series of studies, we demonstrate how substrate interactions impact both the response to an oncogene and the cellular contractility and organization of a monolayer. We first examine the effects of …
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Microfluidic Methods for Investigating Cell Migration and Cell Mechanics
… topology of the environment that surrounds the cell. Additionally, I sought to develop a microfluidic device able to measure mechanical properties of single cells with a sufficient throughput to account for the inherent heterogeneity of biological samples. Throughout this thesis I made use of …
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Cancer Cell Mechanics in Chemoresistance and Chemotherapeutic Drug Exposure
… is complex, recent studies have highlighted that cellular structure and extra-cellular composition, mechanics and structure play a role in the development of chemoresistance. The mechanical properties of cells impact their architecture, migration patterns, intracellular trafficking and many other …
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Cell Mechanics: From Cytoskeletal Dynamics to Tissue-Scale Mechanical Phenomena
<p>This dissertation explores the mechanics of living cells, integrating the role of intracellular activity to capture the emergent mechanical behavior of cells. The topics covered in this dissertation fall into three broad categories : (a) intracellular mechanics, (b) interaction of cells with the …
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Experimental and Computational Analysis of Cell Mechanics during Spreading and Migration
The processes of cell migration and spreading are critical for wound healing and cancer metastasis. Cells use their ability to generate mechanical forces to probe the mechanical resistance of their environment and move forward. Biomechanical explanations for how mechanical factors in their …
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Aging Changes Cell Mechanics and Dynamics with a Backbone of Cytoplasmic Crowding
… effect of aging could be better understood in cells, which are the fundamental building blocks of life. In particular, because the cytoplasm comprises approximately 70% of cells and its physical properties strongly influence biological processes, understanding its mechanics provides a more …
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Cell mechanics-based modelling and in toto imaging describe a new mode of lumen formation in chicken neural tube
… through secondary neurulation where the caudal cell mass forms multiple de novo lumens that then merge to form a central lumen. Second neurulation is also thought to be present in several other species, such as mouse and human, whilst zebrafish takes a different approach of lumen opening that …
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The Functional Roles of Rho-Kinase and Matrix Metalloproteinases in Regulating Corneal Stromal Cell Mechanics in 3-D Collagen Matrices
The file named "ZHOU-DISSERTATION-2013.pdf" is the primary dissertation file. Eleven (11) supplemental movie files are also available.
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Mechanotransduction at cell-cell contacts
… whether the differences in cadherin- dependent cell-cell adhesion energies could solely predict cell sorting. Although adhesion energies do influence cell sorting in vitro, recent studies suggested that that differences in cell adhesion, determined mainly by cadherin binding affinities and …
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Dynamics of Cell Packing and Polar Order in Developing Epithelia
… functionality form from a single fertilized egg cell. Mechanisms that control shape, size and morphology of tissues pose challenges for developmental biology. These mechanisms are tightly controlled by an underlying signaling system by which cells communicate to each other. However, these …
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First-passage time analysis of particle transport in the cytoplasm
Cell mechanics are often probed by tracking fluorescent tracer particles embedded in the cytoplasm. The analysis of such experiments typically involves computation of the mean-square displacement of the particles, and thus ignores the variation in how individual particles are transported by …
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Modeling vascular smooth muscle contraction
… of (1) a model of single vascular smooth muscle cell membrane electrophysiology, contractile system kinetics and cell mechanics; (2) a model of signal transduction of nitric-oxide induced vascular smooth muscle relaxation, and (3) a cellular-based model for myogenic responses of isolated …
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Mechanical characterisation of mammalian cells during monolayer formation using atomic force microscopy
Exploring the elasticity of mammalian cells - through interpretation of the Young’s elastic modulus [E] - has become one of the most useful benchmark biomarkers for investigating the relationship between the physiological and mechanical properties of cells. Such investigations have, until now, …
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Fluorescence laser tracking microrheology for quantitative studies of cytoskeletal mechanotransduction
To shed light on the cell's response to its mechanical environment, we examined cell rheology at the single cell level and quantified it with nanometer spatial and microsecond temporal resolutions over a five-decade frequency range (- 0.5 Hz to 50 kHz). To this end, we developed and optimized an …
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Technology assessment and feasibility study of high-throughput single cell force spectroscopy
In the last decade, the field of single cell mechanics has emerged with the development of high resolution experimental and computational methods, providing significant amount of information about individual cells instead of the averaged characteristics provided by classical assays from large …
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Fabrication of topographically patterned hydrogel substrates and their influence on cell morphology
Cell behavior is influenced by a multitude of extracellular factors including chemistry, mechanics, and geometry. Engineered microenvironments can be used to assess a wide range of such influences on cell behavior and identify parameters for future implementation into tissue engineered scaffolds. …
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Intranuclear strain measured by iterative warping in cells under mechanical and osmotic stress
… and regulation center for gene expression in the cell. Mechanical forces transfer to the nucleus directly and indirectly through specific cellular cytoskeletal structures and pathways. There is increasing evidence that the transferred forces to the nucleus orchestrate gene expression activity. …
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E-cadherin mechanotransduction beyond cell-cell junctions
… and integrin receptors form crucial extracellular adhesive connections with, respectively, adjacent cells and the extracellular matrix, and transduce mechanical signals across cell membranes. The role of adhesive complexes in diseases, cardiomyopathies, atherosclerosis, cancer, stem cell …
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Mechanochemical pattern formation in the cellular actomyosin cortex
Protein patterning is essential for cellular function. From cell division to cell migration, specific positional and temporal arrangement of proteins are requisite to triggering and executing vital cell processes. In the aforementioned examples and many other fundamental cellular activities, …
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