Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Cell cycle stage"”.
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A toolset for linking phenotype and gene expression at the single-cell level
The development of single-cell RNA-sequencing has led to a new degree of resolution in the characterization of complex, heterogeneous biological systems. However, existing methods are often limited in their ability to link these whole-transcriptome profiles with complimentary measurements of …
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Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… is a standard model system for analyzing cellular response as it is related to the cell cycle. The analysis of yeast cell cycle is typically done visually or by using flow cytometry. The first of these methods is slow, while the second offers a limited amount of information about the …
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The Mitotic Exit Network detects spindle position and anaphase entry
… (1) movement of the nucleus into the daughter cell and (2) anaphase onset. These two events only coincide after anaphase chromosome segregation, ensuring that mitosis is complete prior to cytokinesis. The MEN is regulated by spindle position. The MEN GTPase, Tem1, is inhibited as long as the …
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Mathematical modeling of molecular mechanisms governing cell cycle progression in Caulobacter crescentus and differentiation of immune system progenitor cells
… the underlying molecular mechanism that drives cell fate determination in granulocyte-monocyte progenitor (GMP) cells that are exposed to various cytokines. By converting this molecular mechanism into a set of ordinary differential equations (ODEs), we acquired new insights into the behavior of …
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Genome-wide microscopy screening identifies links across processes including a conserved connection between DNA damage control and the microtubule cytoskeleton
… high-content microscopy screening of a cell library consisting of over 3000 single mutant strains of the fission yeast, Schizosaccharomyces pombe. Each strain has one nonessential gene knocked-out, allowing investigation of the resulting phenotypes. I report the implementation and …
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Mechanisms of Regulation of Mitochondrial Dynamics by the Actin Cytoskeleton
… mitochondrial partitioning between daughter cells in mitosis. Propagating in the space surrounding mitochondria is a wave of actin polymerization and depolymerization. In control and wave-depleted metaphase cells we labeled mitochondrial subpopulations with photo-activatable GFP and tracked …
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The role of transition fibre proteins in regulating CEP164C assembly and localisation to the flagellum in the African Sleeping Sickness parasite Trypanosoma brucei
… bodies, essential for flagellum assembly and cell division in the procyclic cycle.. In particular, CEP164C is thought to be a key organiser of the transition fibres and an integral part of the “Grow and lock model”, which prevents the old flagellum from growing and directs resources towards …
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Morphological and Neuroanatomical Changes Associated with the Induction of Neurodegeneration and Life Span Reduction in Drosophila melanogaster by Glia Targeted Expression of Cdh1/Rap/Fzr, An Activator of the Anaphase Promoting Complex
… Complex/Cyclosome (APC/C). APC/C facilitates the cell cycle stage-specific degradation of cyclins and other mitotic regulators, resulting in the inactivation of Cdk (Cyclin Dependent Kinase) complexes, thus promoting completion of mitosis. Previous studies from our laboratory have shown that …
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The effects of altered membrane fatty acid composition on the toxic interactions of heavy metals with Saccharomyces Cerevisiae
… 65% and 40% of the total fatty acids in whole-cell and plasma membrane lipids, and nuclear membrane lipids, respectively. Incorporation of the exogenous PUF As had no discernible adverse effects on cell division. However, inhibition of cell division in the presence of Cd(N03)2 was accentuated …
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Prediction of mRNA polyadenylation sites in the human genome and Mathematical modeling of alternative polyadenylation
… plays many important roles in the cell, such as transcription termination, mRNA stability and transportation, and mRNA translation in eukaryotic cells. A large number of human and mouse genes have multiple polyadenylation sites (referred to as poly(A) sites) that lead to variable …
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Control of M-G1 phase-specific expression in fission yeast
The mitotic cell cycle underlies propagation of eukaryotic cells, continually duplicating and dividing. The past few years have seen major advances in understanding of the regulatory mechanisms that impose on the cell cycle to tightly co-ordinate progression through its individual phases, …
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Measuring ongoing chromosomal instability in single-cell DNA sequencing data
… computational tool to correctly estimate single-cell ploidy and replication status. Cancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy as a consequence. Correct estimation of the ploidy of single cell genomes is crucial for many aspects of downstream …