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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"Cell culture systems."”.
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High density animal cell culture systems using porous supports
… examined as supports for large scale mammalian cell culture. Vero cells and transformed baby hamster kidney (BHK) cells which produced human transferrin were grown in three different reactor configurations: a fixed bed ceramic perfusion system, an airlift systemwith draft tube made of porous …
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Expression of anti-HIV peptides in tobacco cell culture systems
Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2009.
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Investigation of the antioxidant activity of phenolic compounds in cell culture systems
… compounds in ultraviolet radiation-exposed cultured skin cells. Biocatalytic synthesis of polyphenolic compounds was carried out using isolated enzymes, tyrosinase from Agaricus biosporus, and laccase from Trametes pubescens to produce oxidized oligomeric products.
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Designing quantitative structure activity relationships (QSAR) to predict specific toxic endpoints for polybrominated diphenyl ethers (PBDE) in mammalian cell culture systems.
… toxic endpoints for PBDEs in mammalian cells. QSAR modeling uses the known chemicals' activity, which serve as surrogates to study unknown chemicals belonging to the same family. This research is a threefold process including running in-vitro bioassays to collect data on the toxic …
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Microfluidic platforms to evaluate the effects of drugs on fertility and proteins on cancer progression – a focus on cisplatin, alpha-smooth muscle actin, and glial cell line-derived neurotrophic factor
The introduction of in vitro systems that can mimic organ physiology and allow studying the progression of diseases such as cancer is necessary to get reliable information about cell responses and test drug efficacy and toxicity. Classical cell culture systems facilitate a wide range of clinical in …
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Selektion, Charakterisierung und Entwicklung von neuronalen Subtypen des optischen Tektums des Huhns in vitro
It is possible to cultivate cells of the optic tectum of the chick and to observe significant processes of growth and differentiation under serum free conditions. Visual analysis allowed a first classification of the cells in different cell types. Immuncytochemical and electrophysiological …
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The Design of Polyelectrolyte Multilayers Using Galactosylated Chitosan
… in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. Hepatocytes have a surface protein, called the asialoglycoprotein receptor …
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The interplay of matrix rigidity and mechanical stretching for the development of artificial skin
… employed to improve tissue morphogenesis, and cellular spatial organization but it has not been previously implemented in a synergistic manner with matrix rigidity due to lack of tools in 3D cell culture systems. The goal of this study is to investigate the structural changes, the morphological …
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Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
… cases of chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Significant disadvantages exist among currently available therapeutics (e.g. IFNca, lamivudine, adefovir, etc.) that include limited efficacy and the promotion of drug-resistant viral strains. These therapeutics are the research …
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Functional Expression of ABC Transporters at Blood-tissue Barriers: Relevance to Antiretroviral Drug Tissue Distribution
… at the plasma membrane in primary human Sertoli cells and mouse Sertoli cell culture systems using quantitative polymerase chain reaction, immunoblotting and confocal immunofluorescence microscopy, respectively. In mouse Sertoli cell culture systems, using radiolabeled and fluorescent substrates …
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Investigations into the Role of Paf1 Complex Proteins in Drosophila Ovaries
… and chromatin modifications. The ability of two cells with identical genomes to have entirely different transcriptomes and therefore cellular behaviors has piqued the curiosity of many researchers creating the whole field of Chromatin Biology. The difference in behavior of otherwise identical …
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DEVELOPMENT OF TRAPPING STYLE CASSETTES FOR NEW GENE TARGETING STRATEGIES
… of homologous recombination in mammalian cell culture systems. Later in the 1990s, innovative gene trapping strategies were developed to enabled random mutagenesis. Today, the goal is to generate more versatile tools to avoid limitations posed by these earlier mutagenesis strategies. Many …
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A Cell-Based Model to Study Factors that Drive Diffuse Astrocytoma Development
… a hypermethylator phenotype that prevents the cells from differentiating. Acquisition of additional mutations in tumor suppressors such as p53 and/or ATRX likely leads to tumor initiation. Work in the Wells Laboratory has found that loss of the HIPPO adaptor protein AmotL1 is also associated …
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Analysis and implementation of the bilayer microfluidic geometry
… sciences. In particular, they have been used in cell culture systems, where they are used to monitor cell behavior in various environments. One challenge that has emerged, however, is the ability for a microfluidic device to uniformly deliver soluble factors to a given culture of cells without …
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Profiling muscle stem cell adhesion using extracellular matrix peptide arrays
… of life and increases pressure on healthcare systems. Declining skeletal muscle (SkM) mass and function coupled with a reduced tissue healing capacity is one of the main drivers of loss of independence in the elderly. Our previous work revealed that aged SkM contains lower levels of certain …
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Traumatic Injury-Induced Cell Responses in Human Cortical Organoids
… This is driven by secondary injury cascades of cellular toxicity, inflammation, and aggregation of toxic proteins. Although animal models and reductionist human cell culture systems provided important mechanistic insights, there is no available therapy to prevent pathological progression, which …
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Human Parainfluenza Virus 3: Genetic Diversity, Virulence and Antiviral Susceptibility
… on HPIV3 has been conducted using significantly culture adapted reference strains. Therefore, minimally adapted clinical strains were grown in two cell culture systems: immortalised and primary. Plaque phenotype, growth kinetics and inflammatory response triggered were evaluated and it was found …
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Characterization and application of large disposable shaking bioreactors
… for cultivation of plant, animal and insect cell cultures for pilot-scale production. Cylindrical reactors of size 2L, 20L and 50L were thoroughly characterized in terms of important engineering parameters such as mixing, power consumption, heat transfer rate and oxygen transfer rate. …
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