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Showing 1 to 20 of 1083 for “"Cell culture"”.
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Microscale controlled continuous cell culture
Measurements of metabolic and cellular activity through substrate and product interactions are highly dependent on environmental conditions and cellular metabolic state. For such experiments to be feasible, continuous cultures are utilized to ensure consistent conditions. However, since medium must …
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Fabrication of Micropatterned Hydrogels for Cell Culture
… agent and have the characteristics of extracellular matrix. Therefore, they are suitable for cell growth and appropriate for forming cell culture. Hydrogels can mimic the cell microenvironments and promote cell differentiation by interactions with cells. Cells can get oxygen, nutrients …
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Mathematical Modeling of Perifusion Cell Culture Experiments
<p>In perifusion cell cultures, the culture medium flows continuously through a chamber containing immobilized cells and the effluent is collected at the end. In our main applications, gonadotropin releasing hormone (GnRH) or oxytocin is introduced into the chamber as the input. They stimulate the …
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Metabolic flux analysis in mammalian cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1993.
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Microfluidics for Cell Culture and Single Cell Assays
Elucidating molecular processes that govern cell function is paramount in forming a basis for understanding human health and treating disease. Research is presently limited by investigating in bulk, where information on critical subsets of cells is lost through averaging across an entire …
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In vitro models for airway epithelial cell culture
… of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear stress, stretch, the air-liquid interface and the biochemical factors in the medium influence the biology of the cells. The aim of this work is …
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High density animal cell culture systems using porous supports
… examined as supports for large scale mammalian cell culture. Vero cells and transformed baby hamster kidney (BHK) cells which produced human transferrin were grown in three different reactor configurations: a fixed bed ceramic perfusion system, an airlift systemwith draft tube made of porous …
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Cryptosporidiosis in the Immunosuppressed Mouse and in Cell Culture
… (AIDS). Persons with abnormal humoral or cellular immune function, and even normal subjects, have also been affected.</p> <p>To determine whether or not mice, when experimentally immunosuppressed, would develop cryptosporidiosis, a test was designed using the AKR/J strain. This strain was …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
Cell behavior is strongly influenced by the microenvironment. In vitro studies have conclusively shown that chemistry, geometry, and mechanics have all been shown to influence or direct cellular proliferation, differentiation, and matrix formation. However, few systems exist that allow researchers …
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Packed fiber bed reactor design for animal cell culture
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1987.
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Extracellular Matrix-Derived Microcarriers as 3-D Cell Culture Platforms
Recognizing the cell-instructive capacity of tissue-specific extracellular matrix (ECM) to direct cell attachment, proliferation and differentiation, there is a need for the development of in vitro cell culture models that reflect the complexity of the ECM to improve stem/progenitor cell expansion …
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Expression of anti-HIV peptides in tobacco cell culture systems
Submitted in fulfilment of the requirements for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2009.
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Peptide hydrogels as novel scaffolds for dental pulp cell culture
… tissue regeneration should mimic the native extracellular matrix of tissues and allow recruitment and retention of stem cells whilst also promoting tissue organization and function. To achieve this, biomaterials used for scaffold fabrication should be biocompatible and allow attachment of cells …
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Enzymatic crosslinking of dynamic hydrogels for in vitro cell culture
Stiffening and softening of extracellular matrix (ECM) are critical processes governing many aspects of biological processes. The most common practice used to investigate these processes is seeding cells on two-dimensional (2D) surfaces of varying stiffness. In recent years, cell-laden …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… antibody production process, including upstream cell culture, downstream purification and analytical characterisation. A CHO-DP12 cell line engineered for anti-IL-8 mAb expression and a cNIST mAb-producing CHO cell line were employed to investigate key cell culture process parameters. Upstream …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
… tissues and frequently combines therapeutic cells, biomaterial scaffolds, and therapeutic molecules in efforts to do so. One of the primary considerations in such therapies is to orchestrate the interactions between these components to regulate the desired cellular responses. This thesis …
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Microenvironmental modification and analysis of 3D cell culture using microgels
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
Pluripotency – the ability of cells to differentiate into all three germ layers – is of great interest, particularly in the fields of developmental biology and medicine. The in vivo continuum can be separated into three in vitro states that are also found in the embryo: naïve, formative, and primed …
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Glycosylation site occupancy heterogeneity in Chinese hamster ovary cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
… were used to develop relations between mammalian cell physiology and specific culture environments and to formulate strategies for increasing cell culture performance. Mitochondrial characteristics associated with respiration, membrane potential, and apoptosis along with physiological state …
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