Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Cell contractility"”.
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
… controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the …
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Effects of the MYH7 R369Q Dilated Cardiomyopathy-causing Mutation on Myosin Crossbridge Kinetics and Cardiomyocyte Contractility
… within a cardiomyocyte that results in hypocontractility. To better understand the biochemical and biophysical effects of R369Q mutation, this study utilized CRISPR/Cas9-edited R369Q homozygous human induced pluripotent stem cell-derived cardiomyocytes to investigate its effect on the myosin …
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STEP-enabled Force Measurement Platform of Single Migratory Cells
… are demonstrated for the measurement of group cell migration for wound closure and single cell contractility force for the study of force modulation. The wound healing assay bridges the gap between confluent reservoirs of NIH3T3 fibroblasts through arrangement of a suspended array of fibers …
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Inhibition of myofibroblast contraction
… electrical conduction. Layers of contractile cells known as myofibroblasts have been observed around regenerated nerve portions. An inverse relationship between the layer thickness and the quality of the regenerate has also been observed. These findings suggest that the cells are exerting …
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MECHANOBIOLOGICAL REGULATION OF ATRIOVENTRICULAR VALVE MORPHOGENESIS
… ligand signaling programs to orchestrate cellular activities that bring about valve morphogenesis. The objective of this thesis was to elucidate the influence of mechanical stress on the biology of valve embryonic cells to determine how mechanical stimuli regulate normal development and …
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E-cadherin mechanotransduction beyond cell-cell junctions
… and integrin receptors form crucial extracellular adhesive connections with, respectively, adjacent cells and the extracellular matrix, and transduce mechanical signals across cell membranes. The role of adhesive complexes in diseases, cardiomyopathies, atherosclerosis, cancer, stem cell …
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Mechanochemical pattern formation in the cellular actomyosin cortex
Protein patterning is essential for cellular function. From cell division to cell migration, specific positional and temporal arrangement of proteins are requisite to triggering and executing vital cell processes. In the aforementioned examples and many other fundamental cellular activities, …
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Differentiation Induces Dynamic Alterations in Mesenchymal Stem Cell Nuclear Architecture and Mechanotransduction
Mesenchymal stem cells (MSCs) are a promising cell source and widely used in a variety of regenerative applications given their multipotent nature. MSCs are subjected to various types of mechanical forces during tissue development and repair, and it is clear that, along with soluble factors, …
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Cells and force transduction
… at cadherin complexes is not restricted to cell-cell junctions but is also propagated globally via the mediation of its respective RTK binding partner. These results suggest that homophilic ligation in trans- and cadherin association with cognate receptor tyrosine kinase in cis comprises a …
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Dissecting combinatorial microenvironmental regulation of cell fate and function using a multi-modal arraying platform
… the microenvironmental cues which guide cell fate and function. These cues can include biochemical elements, as in ligand–receptor binding for cell–cell or cell–extracellular matrix (ECM) interactions, or responses to biophysical parameters, as in cell sensing of substrate material …
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Development and application of chemical tools for investigating dynamic processes in cell migration
Cell migration is a dynamic process essential for many fundamental physiological functions, including wound repair and the immune response. Migration relies on precisely orchestrated events that are regulated in a spatially and temporally controlled manner. Most traditional approaches for studying …
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Antagonistic Roles of miR-199a-3p/miR-214 and the miR-200 Family in the Regulation of Uterine Contractility During Pregnancy and Labor
… roles in regulating myometrial quiescence and contractility during pregnancy and labor (Kamel et al., 2010). While these contrasting hormonal effects are likely mediated via differential regulation of inflammatory and contractile genes, the underlying mechanisms remain incompletely understood. …
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Continuum Models of Collective Migration in Living Tissues
… the physical mechanics of collective cell migration in monolayers of epithelial cells. Coordinated cell motion underlies a number of biological processes, including wound healing, morphogenesis and cancer metastasis, and is controlled by the interplay of single cell motility, cell-cell …
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Determining Mechanisms by which Pathological Extracellular Matrix induces Ocular Hypertensive Phenotypes in Human Trabecular Meshwork Cells
Purpose: Aberrant extracellular matrix (ECM) remodeling is prevalent in several sight-threatening diseases; in the trabecular meshwork (TM), it is implicated in elevated intraocular pressure (IOP), the only treatable risk factor for glaucoma. Herein, two different glaucoma-relevant cell-derived …
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Monitoring single heart cell biology using lab-on-a- chip technologies
… structures for the analysis of single cells; however, substantially less work has focused on developing "active" assays, where the cell‘s metabolic and physiological function is itself controlled on-chip. The heart attack is considered the largest cause of mortality and morbidity in the …
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Single Cell Force Platforms to Link Force-ECM Coupling in Pathophysiology
Migratory cells in vivo move within a predominantly fibrous microenvironment through the action of forces. These dynamic interactions facilitate mechanosensing, critical to fundamental biological processes in pathophysiology. Naturally, the field of mechanobiology has evolved over the past several …
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Mechanical modulation of indirect repair mechanisms for improved hematopoietic recovery
Hematopoietic stem cell or bone marrow transplantation is a curative treatment for multiple hematologic malignancies. However, the myeloablative conditioning regimens preceding cell delivery have rendered the rapid and sustained hematopoietic recovery after transplantation an outstanding challenge. …
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Keratose Hydrogels Promote Vascular Smooth Muscle Differentiation from c-kit+ Human Cardiac Stem Cells: Underlying Mechanism and Therapeutic Potential
… limit this therapy. C-kit+ human cardiac stem cells (hCSCs) offers a viable alternative therapy to treat cardiovascular disease by replacing damaged cardiac tissue; however, low cell viability, low retention/engraftment, and uncontrollable in vivo differentiation after transplantation has …