Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 134 for “"Cell analysis"”.
-
Single cell analysis of Keap1-Nrf2 dynamics
… protein which allows Nrf2 to respond to changing cellular conditions. In the basal state, Nrf2 binds to two sites of a Keap1 dimer allowing its ubiquitination in a Cullin-3/Rbx1-dependent manner. In response to electrophiles and oxidants (termed inducers, which bind directly to Keap1) …
-
Single Cell Analysis of the HIV-1 Latent Reservoir
… of rare, long-lived, latently infected cells. These cells, called the latent reservoir, are thought to resist immune clearance and viral cytopathic effects by harboring a transcriptionally quiescent integrated HIV-1 provirus. As a result, interruption of suppressive therapy almost …
-
Single Cell Analysis of B Cell Responses to Malaria
… advances in technologies such as single cell RNA sequencing are allowing scientists to studying many of these aspects in ways never attempted before. It is known that the generation of a functional germinal centre (GC) to produce affinity matured long lived plasma cells (LLPCS) and memory …
-
Single Cell Analysis of B Cell Responses to Malaria
… advances in technologies such as single cell RNA sequencing are allowing scientists to studying many of these aspects in ways never attempted before. It is known that the generation of a functional germinal centre (GC) to produce affinity matured long lived plasma cells (LLPCS) and memory …
-
Massively multiplexed imaging probes for comprehensive single-cell analysis
… microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular …
-
Development of High-Throughput Platforms for Single-Cell Analysis
… deal with highly heterogeneous populations of cells where the contributions of individual players cannot be ignored. Single-cell technologies aim to resolve this heterogeneity by analyzing many individual cells in a high-throughput manner. Here we developed two examples of such tools that rely …
-
Development of a Dielectrophoresis-Based Cancer-Cell Analysis Tool
… fields in a microfluidic device to separate cells by biophysical phenotype. By optimizing force balancing between the dielectrophoretic force and the drag force on cells in the device, and by using cell-size pillars to maximize electric field gradients per volt applied while reducing …
-
Automated hydrodynamic trap for single cell analysis in free solution
… to study inducible gene expression at the single cell level, albeit without the ability to control external stimuli with precise methods. Most are limited by long diffusive timescales to alternate environmental concentrations. In this work, we report a microfluidic-based platform for single cell …
-
Integrated, single-cell analysis of transcriptional phenotype and clonotypic identity
Single-cell RNA sequencing (scRNA-seq) currently affords the ability to resolve whole transcriptomes of single cells with substantial throughput and has revolutionized studies of gene expression. Recent technical advances have enabled the matching of T cell receptor (TCR) and B cell receptor (BCR) …
-
Passivated-Electrode Insulator-Based Dielectrophoretic Chips for Rare Cell Analysis
The analysis of potentially harmful biological particles is imperative for the mitigation of disease. As a result, there is a growing need for tools which can characterize, detect, and separate biological particles for the alleviation of a multitude of disease. One powerful technique for the …
-
Single-cell Analysis of Structural and Immune Cell Interactions in Periodontitis
… insight into inflammatory pathways, single-cell RNA sequencing (scRNA-seq) enables detailed characterization of cell-type–specific responses and coordinated interactions within the periodontal microenvironment. OBJECTIVE: This study aimed to compare the transcriptional landscapes of …
-
Development of enabling technologies for single cell analysis with mass spectrometry
… robust performance. A growing field of single cell chemical analysis has arisen around these figures of merit. While early reports utilized manual isolation and extraction, recent developments in high-throughput sampling have enabled the examination of large populations of cells. One such …
-
High throughput microfluidic technologies for cell separation and single-cell analysis
The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has …
-
Label-Free Microfluidic Devices for Single-Cell Analysis and Liquid Biopsies
… efficient, and have rapid processing and analysis. Microfluidic platforms in cancer research have proven to be advantageous due to its operation at the microscale, which has low costs, favorable physics, high precision, short experimentation time, and requires minimal reagent and sample …
-
Phytoplankton growth and diel variations in beam attenuation through individual cell analysis
… of phytoplankton, from flow cytometric analysis of individual cells, was determined and found to show similar diel patterns for populations of pico- and nanophytoplankton measured in the equatorial Pacific and the Sargasso Sea. The cell concentration patterns do not generally correspond …
-
Single cell analysis of bacterial communication and gene transfer by Enterococcus faecalis
… resistance and transfer between E. faecalis cells is facilitated by cell-to-cell communication. This signaling triggers expression of genes from pCF10 that encode for transfer machinery. The response to signaling is robust and has been extensively studied at the population level. However, it …
-
Single cell analysis by capillary electrophoresis with laser-induced native fluorescence detection
<p>Individual mammalian cells were analyzed by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This technique was chosen due to its high separation efficiencies, small sample volumes and sensitive detection. Native fluorescence was used, in which the analyte was not …
-
Development of new photoluminescent sensing materials, systems and applications for cell analysis
Cell analysis using optochemical sensors represents large and important niche in life and biomedical science. Sensor calibration and detection platforms are critical during sensor fabrication, characterization and application. However, existing calibration setups, platforms, and systems have …
-
A genome-wide, single-cell analysis of vascular smooth muscle cell plasticity
Vascular smooth muscle cells (VSMCs) possess a remarkable capacity to change phenotype in response to injury or inflammation. In healthy arteries, VSMCs exist in a contractile state, but upon vascular inflammation or injury, they can switch into an activated state, in which they downregulate the …
-
Phytoplankton growth and diel variations in beam attenuation through individual cell analysis
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
Page 1 of 7