Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 2007 for “"Cell Line"”.
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Engineering mammalian cell line to improve sialylation
… of sialidase released to supernatant when the cell starts to die. This phenomenon is undesirable because the loss of terminal sialic acid results in a product which is rapidly removed from the plasma by the interaction with asialoglycoprotein receptors in the liver. Many studies have been done …
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Pancreatic peptide hormone expression in the RINm5F cell line
… is a striking feature of the pancreatic islet cell lines (RIN and MSL) derived from a rat islet cell tumour (Chick et al., 1977) which may therefore be suitable for the study of islet cell differentiation in vitro. In the present study, the RINm5F cell line (Oie ~-, 1983) was investigated to …
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Studies on intracellular calcium pools in a pancreatic cell line
… (IP₃) can release Ca²⁺ from intracellular stores, the phosphatidylinositol signal transduction system has been recognized as a major transduction pathway in most cell types. However, IP₃ is not the only intracellular Ca²⁺ mediator. In some cells, guanosine 5'-triphosphate (GTP) and …
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Studies on the Complement Receptor of a Human Lymphoblastoid Cell Line
<p>A human lymphoblastoid cell line, 8866, was used to investigate a surface marker, the C3d receptor. A method for cloning these cells at a relatively high efficiency was developed and C3d receptor bearing (CR+) and receptor free (CR-) subclones isolated. Receptor bearing cells were identified by …
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Induction of Marek's disease virus antigens in a lymphoblastoid cell line
Thesis (M.S.)--Michigan State University. Dept. of Microbiology and Public Health, 1975
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RESPONSE OF A NEIL1 DEFICIENT MURINE EPITHELIAL CELL LINE TO CHROMATE
… and the ease with which it can be taken into a cell. Chromate, Cr(VI), ions are taken up by a cell through relatively non-selective anion channels which normally regulate uptake of phosphate and sulfate ions. Once in the cell, chromate ions are reduced by intracellular reductants such as …
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RESPONSE OF A NEIL1 DEFICIENT MURINE EPITHELIAL CELL LINE TO CHROMATE
… and the ease with which it can be taken into a cell. Chromate, Cr(VI), ions are taken up by a cell through relatively non-selective anion channels which normally regulate uptake of phosphate and sulfate ions. Once in the cell, chromate ions are reduced by intracellular reductants such as …
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Exploring mutational signatures in human cancers using human cell line models
… gene knockouts in various isogenic human cell line models (i.e. HAP-1 cells and human-induced pluripotent stem cells) and using whole-genome sequencing as readouts to study their associated mutational patterns. In doing so, I have successfully validated some of the most widely-applied …
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Data-driven predictive modeling for cell line selection in biopharmaceutical production
… development cycle is the selection of the cell line that will become the Master Cell Bank for product manufacturing for clinical and commercial use. This cell line selection process is resource-intensive, requiring several months, involving hundreds of cell cultures and corresponding …
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Intervention of non-small cell line cancer oncogenic pathways with delta-tocotrienol
<p>Non-small cell lung cancer (NSCLC) is most hostile and leading cause of cancer deaths in the United States. Clinical data has demonstrated that 30 % of NSCLC patients have increased Notch activity and 10% of NSCLC have gain-of-function mutation on Notch-1 gene. Our data demonstrated that …
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Elucidation of the Mechanism of Action of a Cell Line Selective Toxin
… therapies is selective toxicity among cancer cell lines. We evaluated results from a viability screen of over 200,000 small molecules to identify two chemical series, oxalamides and benzothiazoles, that were selectively toxic to the same four of 12 lung cancer cell lines at low nanomolar …
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SENSITIZATION OF HUMAN OSTEOSARCOMA CELL LINE 143B WITH CALCITRIOL FOR CISPLATIN THERAPY
… combination with cisplatin on the osteosarcoma cell apoptosis, invasion and migration. Our hypothesis is pretreatment of OS cells with calcitriol would sensitize them for cisplatin therapy leading to decrease in concentration for IC50. Secondary objective was to evaluate the effect of calcitriol …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… unique membrane structures that mediate the intercellular communications among almost all tissues, are composed of connexins in vertebrate. The essential role of gap junction-mediated intercellular communications(GJIC) for coordination of cell homeostasis, tissue functions and organ development …
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Optimisation of CHO Cell Line Development Using Hybrid Modelling for Antibody Therapeutics
… disorders. Chinese Hamster Ovary (CHO) cells are the primary host for mAb production due to their flexible karyotype and human-like post-translational modifications. However, their flexibility poses challenges due to their genetic instability and expression variability upon longer …
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Gene Targeting in a Novel Mouse Model and the Chicken DT40 Cell Line
… genes, followed by transplanting the corrected cells back into the patient. However, the natural rate of gene targeting is too low to be of practical use in most cells; an exception to this is the chicken DT40 cell line which has a high relative rate of gene targeting (gene targeting rate/ …
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Identification of a novel method for differentiating human monocytic cell line into macrophages
… to body repair and signal transfer between cells. Macrophages within tissues are specialised macrophages and adapt their structure and function to optimise fighting foreign material and infections. Part of the immune response involves release of microvesicles (MVs) formed by the outward …
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Chloride Channels in Normal Human Blood Monocytes and a Macrophage-like Cell Line
Phagocytic cells serve critical roles in the body’s immune response by recognizing, phagocytosing and destroying foreign particles. A major class of phagocytic cells is composed of monocytes and macrophages. During inflammation there is release of several inflammatory mediators and chemokines, …
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The Mechanism and Effects of TNF-α on Apoptosis in Auditory Cell Line
… that TNF-α could induce apoptosis in auditory cell line and to investigate the role of nitric oxide (NO) in TNF-α-induced auditory cell death. UB-OC1 cells and zebrafish were exposed to TNF-α. Flow cytometry, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling …
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