Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Cell Culture Techniques"”.
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Rethinking Plant-Based Materials Production: Selective Growth of Tunable Materials Using Cell Culture Techniques
… disruption. This work explores the use of cell culture techniques to selectively generate plant-based materials without whole plant cultivation and harvest. The proposed approach allows for localized, high-density production, elimination of energy-intensive harvest and hauling, and reduced …
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Analyzing GFP-tagged cytoskeletal protein colocalization in human carcinoma cells
… as dynamic, complex components involved in cellular structure and signaling. Characterizing the roles of such proteins would greatly benefit many research areas, including the study of cancer and protein-related diseases. There is currently no accurate, high throughput method of image …
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Cell patterning technology for controlling the stem cell microenvironment
Embryonic stem cells serve as powerful models for the study of development and disease and hold enormous potential for future therapeutics. Yet, over two decades after mouse embryonic stem cells (mESCs) were first isolated, there is still little known about the role of cell-cell signaling in …
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A self-assembling peptide scaffold functionalized for use with neural stem cells
… used synthetic materials employed in the culture of neural stem cells. Furthermore, improvements in the performance of RADA16 have recently been made by appending the self-assembling peptide sequence with various functional motifs from naturally occurring proteins. The focus of this work …
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Cells, surfaces and adhesion
This thesis concerns cell adhesion to polymer surfaces with an experimental emphasis on hydrogels. The thesis begins with a review of the literature and a synthesis of recent evidence to describe the process of cell adhesion in a given situation. The importance of understanding integrin-adhesion …
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A Nonribosomal Peptide Pheromone Controls Male-Induced Female Sexual Development in Schistosoma mansoni
Schistosomes are parasites causing one of most prevalent and serious neglected tropical diseases. Their immense egg output in the host is the main source of pathology, placing their reproductive biology of great importance for scientific researches. A bizarre, and physiologically meaningful …
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The neurobiology of central dopamine and progesterone in the reproductive cycle of the ring dove (Streptopelia risoria)
… Quantitative analysis revealed increased TFJ-ir cells, in male and female brooding doves, in the nuclei periventricularis magnocellularis, dorsomedialis anterior thalami, pretectalis medialis; and in the nucleus dorsomedialis posterior thalami in female brooding birds. This confirms that …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
Pluripotency – the ability of cells to differentiate into all three germ layers – is of great interest, particularly in the fields of developmental biology and medicine. The in vivo continuum can be separated into three in vitro states that are also found in the embryo: naïve, formative, and primed …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… are presently no sources of primary lung stem cells which can be expanded at sufficient scale to permit engineering multiple lungs from a single donor. I have developed a method for conditionally reprogramming primary human bronchial epithelial cells in culture to extend their functional …
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The role of retinoids in the regeneration of the axolotl spinal cord
… has been described in juvenile Axolotl ependymal cells. Retinoid effects may possess a significant role in the regeneration-permissive interaction of the meninges and ependyma of the Axolotl spinal cord after penetrating injury. During spinal cord regeneration in urodele amphibians, the pattern of …
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Biocompatibility and Bioactivity of Calcium Silicate-based Dental Bioceramics
… study used gingival and periradicular fibroblast cell cultures, with both direct and indirect material exposure methods. Several cell culture techniques were used to determine biocompatibility and bioactivity of tricalcium silicate-based materials:<br/><br/>1)Visual observations of cell<br/>2) …
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The anticancer potential of CITme, a quinoline derivative
… for neoplastic cytotoxic effects using standard cell culture techniques revealed cytotoxic activity against HeLa, DU-145, MCF-7, Jurkat and CoLo 320 human derived cancer cell lines at low concentrations. Toxicity was significantly less in either normal resting and stimulated lymphocytes or …