Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Cdk8"”.
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Mechanisms of Transcriptional Control by the Mediator Complex Subunit Cdk8
… factors. Cyclin-dependent kinase 8 (Cdk8) is the only enzymatic subunit in the entire Mediator complex, and it resides in a dissociable module with CycC, Med12, and Med13. Cdk8 regulates transcription by phosphorylating transcription factors in response to environmental signals. …
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Identifying CDK8/19 as a Novel Regulator of EWS-FLI1 Function in Ewing Sarcoma
… molecules that resemble previously described CDK8/19 inhibitors. A role for CDK8/19 in EWS-FLI1 transcriptional activity has not been reported. To determine if the remaining small molecules capable of modulating EWS-FLI1 transcriptional activity were acting through CDK8/19 inhibition I …
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Protein kinase A-dependent phosphorylation and degradation of CDK8 : implications for yeast filamentous growth
… and cAMP pathways. Previous work showed that Cdk8p phosphorylated a key transcriptional activator of the filamentous response, Ste12p, and subsequently targeted the factor for degradation under conditions of limiting nitrogen. Data presented in this thesis suggests that Cdk8p is regulated by …
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Identifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… has determined that Cyclin Dependent Kinase 8 (CDK8) and Mediator Complex Subunit 12 (MED12), components of the kinase module in the Mediator complex, contain consensus sequences for binding to the N-terminus of USP7. Using various co-immunoprecipitation, pulldowns, tissue culture, and western …
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Coordinated of Post-Translational Modifications of Yeast Transcriptional Activator Gcn4
… depends on phosphorylation, by either Pho85 or Cdk8, which triggers its ubiquitination and subsequent proteolysis by 26S proteasome. Previous studies showed that Gcn4 becomes sumoylated at two Lys residues (K50, 58), specifically after binding to target gene promoters. However, it is not clear …
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Functional Analysis of Drosophila Integrator Complex In Snrna 3' End Processing
… found a conserved requirement for Cyclin C and Cdk8 in both fly and human snRNA 3’ end processing. We have further demonstrated that the kinase activity of Cdk8 is critical for snRNA 3’ end processing and is likely to function independent of its well-documented function within the Mediator Cdk8 …
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
… Mam were investigated, with CBP/p300 and the CDK8 module of the Mediator complex recruited to the transcription hub and required for transcription. However, of the two complexes, only the CDK8 module had an influence on Mam recruitment at the target locus. Further investigation suggested the …
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The DNA damage response to ATR inhibition
… and ATM-deficient cells encoded Cyclin C and CDK8, which function as a complex to regulate RNA polymerase II-mediated transcription. We show that Cyclin C/CDK8 loss promotes resistance to inhibitors of the replication stress response (RSR), and reduces S-phase DNA:RNA hybrid formation and …
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Characterization of the Enhancer and Promoter Elements of the Human TAF(II)55 Gene
… The 500 mM fraction-derived Mediator contains Cdk8/Cyclin C, while the 850 mM fraction-derived Mediator lacks the protein pair and likely corresponds to the previously characterized positive cofactor 2 (PC2). The associated RNA polymerase II was also depleted from the Mediator complexes with an …
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Med12 Is A Critical Regulator of Neural Crest Lineage and Nervous System Myelination
… eukaryotic transcription machinery. MED12 is a Cdk8- regulatory kinase module subunit directly implicated in human disease and is genetically altered in neurological disease and cancer. Numerous attempts at generating an<em> in vivo</em> system to study the role of Med12 failed due to embryonic …
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Site-specific phosphorylation of histone H1 associated with cell cycle progression and transcription
… elements of transcribed genes. Our data suggest CDK8 and CDK9, kinases with known association with the transcriptional machinery, as candidates that could account for this distribution of interphase H1 phosphorylation. Comparative analyses of H1 variant expression and phosphorylation in different …
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Molecular and biochemical characterisation of phosphorylation-dependent NICD1 turnover: novel roles for CDK1 and CDK2
… by two kinases namely cyclin-dependent kinase-8 (CDK8) and GSK-3β, which then recruits the SCF Sel10/FBXW7 E3 ubiquitin ligase complex, that targets NICD for degradation by the proteasome. However, many of these studies have been conducted through overexpression assays in vitro and none of this …