Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 53 for “"Cdk1"”.
-
Eukaryotic chromosome segregation: New aspects of separase regulation by securin, Cdk1, PP2A and auto-cleavage
… either securin or cyclin-dependent kinase 1 (Cdk1) in conjunction with cyclin B1. Only when all chromosomes interact properly with the mitotic spindle apparatus does the anaphase promoting complex or cyclosome (APC/C), a multisubunit E3 ligase, mediate the ubiquitylation of securin and cyclin …
-
Molecular and biochemical characterisation of phosphorylation-dependent NICD1 turnover: novel roles for CDK1 and CDK2
In the developing vertebrate embryo, segmentation initiates very early and in a species-specific manner through the formation of somites, which later give rise to the vertebral column, most skeletal musculature and dermis. Somites are gradually and periodically pinched off from the anterior end of …
-
Regulation of mitotic spindle biogenesis in budding yeast
… and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in bipolar spindle formation. In this study, we have investigated the regulatory role of Cdk1 in centrosome separation. We show that E3 ubiquitin ligase …
-
Exploring the Regulation of Mitotic PP2A-Rts1 Activity in Saccharomyces cerevisiae
… by an increase in cyclin-dependent kinase 1 (Cdk1) activity caused by the dephosphorylation of Cdk1 on a conserved tyrosine residue. PP2ARts1 is a phosphatase that participates in dephosphorylating the conserved tyrosine residue, tyrosine-19 (Y19). PP2ARts1 dephosphorylates phosphorylated …
-
Developing 1,2,3,4-tetrahydro-5H-aryl[1,4]diazepin-5-ones and Related Scaffolds as Poly-(ADP-ribosyl) Polymerase (PARP) Inhibitors and Exploring Their Targeted Polypharmacology with Kinases
… lethality of cyclin-dependent kinase 1 (CDK1) and PARP1 is known. Evaluation of PARP1 and CDK1 pharmacophores led to the development of the tetrahydro-arylazepinone (TAAP) scaffold as a potential dual PARP1/CDK1 inhibitor. We screened a handful of TAAP analogs against PARP1 in a cell-free …
-
Meiotic Dna Re-Replication And The Recombination Checkpoint
… nuclear divisions. Cyclin dependent kinase 1 (Cdk1) is required for meiosis, and any disruption in its activity leads to meiotic defects. The Cdk1 inhibitor, Sic1, regulates the G1-S transition in the mitotic cell cycle and the analogous transition in meiosis. We have employed a form of Sic1, …
-
Analysis of the role of rab11-FIP3 phosphorylation during cytokinesis
… show that FIP3 can be phosphorylated by cyclin B-CDK1, Plk1, Aurora A and weakly by Aurora B. A proteomic approach revealed that, within the limits of our experimental approach, only cyclin B-CDK1 phosphorylated FIP3 significantly, at serine 102. Interestingly, proteomic analysis of FIP3 …
-
Mitotic slippage by ATP depletion and its mechanism
… the increase of inhibitory phosphorylation Cdk1 (pY15) by ATP depletion, suggesting the inactivation of Cdk1 kinase during mitotic slippage. Reactivation of Cdk1 during mitotic slippage by treatment with PD166285, a Wee1/Myt1 inhibitor, partially reduced the induction of mitotic slippage by …
-
Investigation of mTOR-independent regulation of macroautophagy
… of CMGC kinase family members ERK1/2 and CCNB1-CDK1 in the regulation of autophagy. The ERK1/2 signalling cascade is activated in a high proportion of cancers. ERK2 has been proposed as a regulatory kinase of TFEB; however, we found little evidence to suggest that ERK1/2 was a direct kinase …
-
Targeting protein kinases to manage or prevent Alzheimer’s disease
… kinases such as cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase 5 (CDK5), glycogen synthase kinase-3 (GSK-3α and GSK-3β), and the protein kinase RNA-like endoplasmic reticulum kinase (PERK). AD intervention by reduction of amyloid beta (Aβ) levels is also possible through …
-
In-depth bioinformatics analysis of the phosphoproteome of triple negative breast cancer treated with a tumor selective NQO1 bioactivatable drug
… signaling of cell cycle checkpoint proteins. CDK1 and CDK2 substrate phosphorylation was decreased in response to combination drug therapy. Based on the differential kinase activity as determined by substrate abundance, we hypothesize that since CDK1/2 plays an important role in DNA damage …
-
Characterisation of the molecular complexes that regulate the G2/M checkpoint of the eukaryotic cell cycle
… or progression is principally mediated by the CDK1/cyclin B1 complex; phosphorylation of CDK1 by wee1 kinase prevents progression to mitosis, and subsequent dephosphorylation by the CDC25 phosphatases, initially the B isoform, leads to mitotic onset. The aim of this research was the biophysical …
-
Mechanisms of cell cycle remodeling at the MBT during the development of Xenopus laevis embryos
… cell cycle arrest. Since XChk1 inhibits both Cdk1 and Cdk2, and GST-D34Xic1 inhibits only Cdk2, we propose Cdk1 destabilizes Wee1, whereas Cdk2 elevates Wee1 level. Prior to the MBT, when cyclin E/Cdk2 is active, Wee1 is maintained. After cyclin E/Cdk2 is destroyed at the MBT, Wee1 is degraded.
-
Function of MCPH1 in Neurogenesis
… temporäre negative Regulation von Cdc25B und Cdk1. Mcph1-defiziente Zellen aktivieren frühzeitig Cdk1 und leiten einen vorzeitigen Eintritt in die Mitose ein, was zu einer Entkopplung des Zellzyklus vom zentrosomalen Zyklus zur Folge hat. Diese Entkopplung führt zu Defekten der mitotischen …
-
Investigating the function of Citron kinase and its regulation by other mitotic kinases
… is regulated by other mitotic kinases, including Cdk1 and Aurora B, suggesting that its functions must be tightly controlled. Furthermore, although several lines of evidence indicate that CIT-K kinase activity is important (the most significant being the identification of mutations in the CIT-K …
-
Regulation of the anti-senescence factor, TBX2, by the UV stress signaling pathway and the mitotic cyclin dependent kinases
… and the mitotic cyclin A/Cdk2 and cyclin B1/Cdk1, are direct regulators of Tbx2 both in vitro and in vivo. It is possible that Tbx2 and Tbx3 may contribute towards the oncogenic process through their anti-senescence function, especially since a dominant negative form of Tbx2 induces …
-
Regulation of the anti-senescence factor, TBX2, by the UV stress signalling pathway and the mitotic cyclin dependent kinases
… and the mitotic cyclin A/Cdk2 and cyclin B1/Cdk1, are direct regulators of Tbx2 both in vitro and in vivo. It is possible that Tbx2 and Tbx3 may contribute towards the oncogenic process through their anti-senescence function, especially since a dominant negative form of Tbx2 induces …
-
The role of Cdc7 and cyclin-dependent kinases in DNA replication and S phase
… in mouse embryonic fibroblasts lacking both Cdk1 and Cdk2 was examined. Contrary to expectations, Cdk1/Cdk2 double knockout cells can enter S phase in the absence of detectable S phase-Cdk activity. S phase progression, however, was inefficient. Cdc6 and cyclin E1 proteins were found to …
-
Investigating the role of cell cycle regulators in mesoderm specification
… CDK4/6, CDK2, Retinoblastoma phosphorylation and CDK1 are essential for mesoderm subtype formation. Furthermore, I have shown that CDK1 regulates the activity of ERK1/2 signalling, an important pathway for the differentiation process confirming the existence of complex interplays between cell …
-
Anti-cancer Functions and Mechanisms of a pRb2/p130 Peptide Fragment
… is also able to inhibit the kinase activities of Cdk1/Cyclin B, Cdk4/Cyclin D and Cdk9/Cyclin T/K. Result of a binding assay using GST-Spa310 and in vitro transcribed/translated Cdk2 did not support a direct binding between Spa310 and Cdk2. Additionally, GST-Spa310 was unable to bind to the in …
Page 1 of 3