Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Cdh1"”.
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Regulation of Anaphase Promoting Complex Coactivators
… evolutionarily conserved coactivators Cdc20 and Cdh1. After an initial wave of APC-Cdc20 activity, APC-Cdh1 degrades multiple mitotic proteins from mitotic exit through G1; inhibitory phosphorylation of Cdh1 by CDK and Polo kinase may allow accumulation of Cdh1 targets in the subsequent cell …
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Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
… the regulation of the APC by its activator Cdh1 in budding yeast in single cells. Inactivation of APC-Cdh1 is an important regulatory transition leading to mitotic entry. I developed and characterized a fluorescent biosensor to measure the dynamics of APC-Cdh1 activity in single cells by …
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Role of Drosophila Rap/Fzr (Cdh1) in retinal axon targeting and its interactions with Loco, Liprin-a and Ras
… mutants. Rap/Fzr is a homolog of mammalian Cdh1, an activator of anaphase promoting complex (APC), an E3 ubiquitin ligase. Previous work has shown that Rap/Fzr is required in cell cycle regulation, glia differentiation and pattern formation during eye development. Results from our …
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Sequence analysis of the E-cadherin (CDH1) gene in a cohort of gastric cancers seen in the Western Cape
… Amongst the genetic factors, the E-cadherin (CDH1) gene is said to play a major role in the development of gastric cancer. Both germline and somatic mutations have been identified in the CDH1 gene. The germline mutations span the entire exon of this gene and are seen in approximately 29% to …
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The regulation of LIV-1 mRNA in MDA-MB-231 human breast cancer cells and its association with E-cadherin (CDH1)
… of the cell adhesion protein, E-cadherin (CDH1), thought to play a role in metastasis. In the present study, we investigated the regulation of LIV-1 and its relationship to CDH1 in MDA-MB-231 ER<sup>-</sup> breast cancer cells. Cells were treated with insulin and EGF for 24 hours and the …
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Regulation of mitotic spindle biogenesis in budding yeast
… separation. We show that E3 ubiquitin ligase APCCdh1 (conserved from yeast to human) acts as a potent inhibitor of spindle assembly by promoting degradation of microtubule-associated proteins essential for centrosome separation. Activated Cdk1 causes inactivation of APCCdh1 during S phase, …
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Strengths and Weaknesses of Hybrid TPR Technology for Obtaining Structural and Mechanistic Insights into TPR Proteins
… the Ile-Arg motifs of substrate coreceptors Cdh1 (or Cdc20) and APC10. Cdh1 and APC10 together recruit substrates for ubiquitination. Therefore, it is important to understand the structure of APC3, and how APC3 mediates interactions. To address this problem, I used a novel “hybrid TPR” …
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Analysis of Aurora B Regulation and Signaling
… system by APC/C that had been activated by Cdh1. The recognition of Aurora B by APC/CCdh1 is specific as it requires the presence of a conserved KEN-box motif at the amino terminus of Aurora B. Degradation of Aurora B at the end of mitosis requires Cdh1 in vivo as the reduction of Cdh1 level …
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Morphological and Neuroanatomical Changes Associated with the Induction of Neurodegeneration and Life Span Reduction in Drosophila melanogaster by Glia Targeted Expression of Cdh1/Rap/Fzr, An Activator of the Anaphase Promoting Complex
… Rap/Fzr (the Drosophila homolog of the mammalian Cdh1) regulates glia differentiation and promotes neuron formation. Recent studies have shown that the glia specific over expression of Rap/Fzr causes progressive neurodegeneration and life span reduction preceded by temperature sensitive paralysis, …
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Mechanism of APC/C activation and substrate specificity in mitosis
… interactions with two coactivators, Cdc20 and Cdh1. In contrast to Cdh1, APC/C activation by Cdc20 during mitosis requires hyper-phosphorylation of APC/C subunits by cyclin-dependent kinase (Cdk) and polo kinase. The aim of the first part of this thesis was to understand how mitotic …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
… via its two WD40-domain co-activators, Cdc20 and Cdh1, which recognise three peptide motifs (degrons) on its substrates: the D-box, the KEN box and the ABBA motif. The regulation of mitotic exit is tightly controlled by modulating the levels of Cdc20 and Cdh1 through different stages of the cell …
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INSIGHTS INTO SUBSTRATE RECOGNITION BY THE ANAPHASE PROMOTING COMPLEX (APC)
… requires binding to one of its co-activators, Cdh1 or Cdc20. The co-activators directly facilitate APC enzymatic activity, but also contribute to the highly selective recognition of substrates via binding to degrons, such as the destruction box (D-box), KEN-box, and ABBA motif. However, not all …
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Temporal organization of the budding yeast cell cycle: general principles and detailed simulations
… by SCF and adaptors of the APC (Cdc20, Cdh1). The emphasis was put on mechanisms responsible for the release of Cdc14 from the RENT complex, Cdc14 being a major player in exit from mitosis. Simulations of the wild type strain and more than 100 mutants showed phenotypes in accordance with …
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Γενετική ανάλυση στον κληρονομικό καρκίνο του μαστού
… BRCA2, CHEK2, PALB2, BRIP1, TP53, PTEN, STK11, CDH1, ATM, BARD1, MLH1, MRE11, MSH2, MSH6, MUTYH, NBN, PMS1, PMS2, RAD50, RAD51C) με την χρήση τεχνολογίας αλληλούχισης επόμενης γενεάς. Για το σκοπό αυτό αναλύθηκαν 42 ασθενείς με σοβαρό ιστορικό καρκίνο μαστού ή/και ωοθηκών, οι οποίες είχαν βρεθεί …
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Regulation of Ciz1 by Cyclin A-Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
… presented demonstrates that an E3 ligase (Cdh1) interacts with Ciz1 via a conserved destruction box in the N-terminus. Taken together, the data presented here suggest that Ciz1 is regulated at multiple levels post-transitionally that contribute to ensuring it accumulates precisely at the …
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Understanding Human Astrovirus from Pathogenesis to Treatment
… of SNAI1 and VIM with a down regulation of CDH1 and OCLN, loss of cell-cell junctions most notably at 18 hours post-infection (hpi), and loss of cellular polarity by 24 hpi. While active transforming growth factor- (TGF-) increases during HAstV infection, inhibition of TGF- signaling does …
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Assessing Genetic Counselors' Clinical Approach and Practices Regarding Pathogenic/Likely Pathogenic Variant Downgrades
… variant downgrade scenarios involving the <em>CDH1</em> or <em>ATM</em> genes with variable family histories. Genetic counselors appear to be united in whether they would discuss how the variant reclassification was reached, the possibility of another reclassification in the future, and …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
… cycles even without undegradable Clb2. The Cdc14-Cdh1-Cdc5 negative feedback is the primary mechanism driving this release oscillator. Its mechanism and regulation of its frequency by Clb2-Cdk, suggest the hypothesis that intrinsically autonomous Cdc14 release cycles are locked at once per cell …
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pH Regulatory Molecules In The Tumour Microenvironment: Modulators Of Aggressiveness And Immune Profile Of Human Hepatocellular Carcinoma
… (EMT) (<i>TWIST, CDH1, VIM</i>) and those encoding for HCC stem cell-associated markers (<i>CD13, CD24, CD44, CD90, EpCAM, CD133, KRT19, OCT4, NANOG</i> and <i>SOX2</i>). Selected HCC, non-tumour and normal liver tissue samples were evaluated by immunohistochemistry …
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