Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Cdc20"”.
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
… of the Anaphase Promoting Complex (APC) protein Cdc20. <em>MAD2</em> encodes a HORMA domain, which I identify as a novel histone binding motif. Mad2 maintains two differing conformations, closed Mad2 (C-Mad2) and open Mad2 (O-Mad2). We find C-Mad2 increases the Mad2-H3 interaction. In addition, …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
… the Anaphase Promoting Complex/Cyclosome (APC/C-Cdc20). TNKS belong to the poly-ADP-ribose polymerases (PARP) family of enzymes that PARylate their substrates, leading to ubiquitination and subsequent degradation. TNKS bind their protein substrates through an ankyrin-repeat domain comprising five …
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Design and development of novel antagonists targeting Cdc20, a central regulator of the Anaphase-Promoting Complex/Cyclosome, for the treatment of cancer
… during mitosis. Cell division cycle 20 (Cdc20), a central SAC regulator and mitotic protein, binds the Anaphase-Promoting Complex/Cyclosome (APC/C), a complex capable of stipulating protein destruction, regulating its activity and driving cell cycle progression through orderly APC/C …
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The Multifunctional Kinase Bub1 Acts as a Signaling Hub for the Spindle Checkpoint
… anaphase-promoting complex or cyclosome (APC/C). Cdc20 is the mitosis-specific APC/C activator. The spindle checkpoint prevents premature sister chromatids separation by preventing Cdc20 from activating APC/C. Bub1 is a highly conserved spindle checkpoint protein that plays multiple roles in …
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Regulation of Anaphase Promoting Complex Coactivators
… by the evolutionarily conserved coactivators Cdc20 and Cdh1. After an initial wave of APC-Cdc20 activity, APC-Cdh1 degrades multiple mitotic proteins from mitotic exit through G1; inhibitory phosphorylation of Cdh1 by CDK and Polo kinase may allow accumulation of Cdh1 targets in the subsequent …
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Regulation of the spindle checkpoint by Mad2 binding proteins
… of Mad2 to the checkpoint protein Madl and Cdc20, a subunit of the Anaphase Promoting Complex. We have characterized the interactions of human Mad2 with Madl, Cdc20, and CMT2, a checkpoint inhibitor. Cdc20 and Madl form competitive high affinity complexes through contacts in the peptide …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… in conjunction with its essential co-activator Cdc20. The spindle assembly checkpoint (SAC) is a surveillance mechanism that monitors the chromosomes' interactions with the microtubules of the mitotic spindle apparatus. In response to even one erroneous attachment the affected kinetochore emits …
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Mechanism of APC/C activation and substrate specificity in mitosis
… by its interactions with two coactivators, Cdc20 and Cdh1. In contrast to Cdh1, APC/C activation by Cdc20 during mitosis requires hyper-phosphorylation of APC/C subunits by cyclin-dependent kinase (Cdk) and polo kinase. The aim of the first part of this thesis was to understand how mitotic …
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Safeguard of Mitosis: The Spindle Checkpoint
… (MCC), which consists of BubR1-Bub3, Mad2, and Cdc20. The diffusible MCC inhibits the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome bound to its co-activator Cdc20 (APC/C-Cdc20) to arrest cells in mitosis. Nevertheless, it remains unknown how the checkpoint monitors …
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Identification and characterization of Sgo2 interactions - Insights into dynamic chromosomal localization, mechanism of cohesin protection and putative checkpoint function
… binding to the downstream effector protein Cdc20. SAC-dependent inhibition of anaphase correlates well in timing with shugoshin-dependent protection of cohesin. Here, Mad2 is identified as another novel interactor of hSgo2. Precise mapping reveal a conserved Mad2 interaction motif (MIM) in …
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
… was used to investigate the expression levels of Cdc20 and Cyclin B1 to confirm the compounds were acting on the intended target. The novel compounds were found to reduce cell viability in the cancer cell lines tested but were also very cytotoxic to the non-carcinomic RPE-1 cells. The cytotoxicity …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… B and securin. The MCC is composed of BubR1, Cdc20 and Mad2, and while their assembly is an intrinsically very slow process, in cells it is catalytically activated. Recent work points towards hierarchical recruitments of SAC proteins onto the outer kinetochore by means of a Mps1-dependent …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in vitro,much more efficiently than Mad2. Surprisingly, the kinase activity of BubR1 or association with Bub3 is not required for the inhibition of APCCdc20. Furthermore, BubR1 restores the mitotic arrest in …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in vitro,much more efficiently than Mad2. Surprisingly, the kinase activity of BubR1 or association with Bub3 is not required for the inhibition of APCCdc20. Furthermore, BubR1 restores the mitotic arrest in …
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Mitotic slippage by ATP depletion and its mechanism
… normal mitotic progression is regulated by APCCdc20, decrease of Cdc20 paradoxically occurred prior to cyclin B degradation during mitotic slippage by ATP depletion. The results presenting the changes in Cdc20 level by MG132 or cycloheximide treatment demonstrated that the decrease of Cdc20 is …
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INSIGHTS INTO SUBSTRATE RECOGNITION BY THE ANAPHASE PROMOTING COMPLEX (APC)
… binding to one of its co-activators, Cdh1 or Cdc20. The co-activators directly facilitate APC enzymatic activity, but also contribute to the highly selective recognition of substrates via binding to degrons, such as the destruction box (D-box), KEN-box, and ABBA motif. However, not all …
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Identification and functional characterisation of novel APC/C interacting proteins
… subunits 1-8, the APC/C‟s mitotic coactivator CDC20 and mitotic substrate cyclin A. Moreover, TIF1\(\gamma\) depletion by RNAi arrests cells in a metaphase-like state characterised by elevated levels of APC/C substrates, cyclin A, cyclin B and CDC20. In support of a role for TIF1\(\gamma\) in …
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CDK control of mitotic progression in Saccharomyces cerevisiae
… a requirement for mitotic CDKs in APC/C-Cdc20 activation in vivo. Second, mitotic CDK activity was necessary for the elongation of the mitotic spindle in anaphase. This requirement for mitotic CDKs is independent of cohesin removal and thus constitutes a novel role for CDK activity in …
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A meiosis-specific form of the Anaphase Promoting Complex/Cyclosome regulates the oocyte-to-embryo transition in Drosophila
… in vitro, while a mitotic APC/C activator, fzy/cdc20, cannot. Mtrm proteins levels are drastically reduced upon completion of meiosis, and this reduction is dependent on cort. When expressed in cell culture, Cort causes a proteasome dependent decrease in Matrimony protein levels. Cort and Mtrm …
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