Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Cdc2"”.
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THE INHIBITOR-OF-APOPTOSIS PROTEIN SURVIVIN INCREASES P34CDC2 PHOSPHORYLATION AND ENHANCES CELL SURVIVAL AND PROLIFERATION BY PROTECTING THE WEE1 KINASE FROM DEGRADATION BY CASPASE-3
… Survivin and the cyclin-dependent kinase p34Cdc2 are involved in cell cycle progression and apoptosis. Activation of Cdc2 is required for its pro-apoptotic activity, which can be inhibited by phosphorylation at Tyrosine-15 (Tyr15). In transduced IL-3-dependent murine BaF3 hematopoietic cells, …
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Part 1 Design, Synthesis and Bioactivity of a Phosphorylated Prodrug for the Inhibition of Pin1; Part 2 Conformational Specificity of Cdc25c Substrate for Cdc2 Kinase using LC-MS/MS
… cell cycle regulatory phosphoproteins, including Cdc25 phosphatase,Cdc27, p53 oncogene, c-Myc oncogene, Wee1 kinase, Myt1 kinase, and NIMA kinas, have been confirmed as substrates of Pin1. Pin1 was also observed to be overexpressed in a variety of cancer cell lines, and the inhibitors of Pin1 …
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MOLECULAR ASPECTS OF DIRECT SOMATIC EMBRYOGENESIS IN ALFALFA (MEDICAGO)
… cyclin genes termed cycMfl and cycMf2 and a cdc2 cDNA fragment from alfalfa. The full cDNA and genomic sequence of cycMfl was determined by the RACE procedure. Several cycMfl 5' end cDNA sequences, representing different allelic variants in the tetrapioid alfalfa genome, were also isolated. …
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The molecular and biochemical characterization of cell cycle regulators in Pisum sativum
… dissertation focuses on the characterization of cdc2-type cell cycle regulators in Pisum sativum (garden pea). Immunoblot patterns indicate that $\sim$34 kDa cdc2-type proteins present in protein complexes may be phosphorylated in pea. As in other systems, these proteins copurify with a histone …
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Identifizierung und Charakterisierung von Interaktionspartnern der Janus-Kinase Hopscotch in Drosophila melanogaster
… Drosophila Cyclin-abhängige Kinase Cdc2 mit Hop sowohl in vivo als auch in vitro interagiert. Ferner wurde belegt, dass Hop Cdc2 in vitro posttranslational durch Phosphorylierung modifizieren kann. Weiterführende in vivo Studien der temperatur-sensitiven konstitutiv aktiven …
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Prolonged T – DC macro-clustering within lymph node microenvironments initiates Th2 differentiation in a site-specific manner
… Th2 cells with migratory type-2 dendritic cells (cDC2s) generating specialized Th2-promoting microenvironments. Macro-clustering was integrin mediated and promoted localized cytokine exchange among T cells to reinforce differentiation, which contrasted behavior during Th1 responses. Unexpectedly, …
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Characterization and Functional Analysis of the Arabidopsis Intron Splicing Factor ASF/SF2
… to be a potential substrate for the mitotic cdc2/cyclin kinase. UV crosslinkage assay demonstrate RNA binding ability of SF2a and RRM domain, but not SF2b protein. Immunostaining of TN-5 cells expressing recombinant proteins and western blot analyzing cytoplasmic and nuclear fractions of …
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Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells
… inhibition of the cyclin-dependent kinase, Cdc2. Both Chk1 and Chk2 have the potential to operate in this pathway. Therefore, the initial aim of this study was to investigate the relative requirement of Chk1 and Chk2 for mitotic delay mechanisms triggered by DNA damage and DNA replication …
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Regulatory Mechanisms of Mitotic Catastrophe Induced by Doxorubicin
… cell cycle profiles using FACS, activities of Cdc2 or Cdk2. RESULTS: LD doxorubicin induced abnormal mitosis and then senescence-like phenotype (SLP) in Huh-7 human hepatoma cell lines. Subsequently, these cells were undergoing cell death through mitotic catastrophe which was caused by abnormal …
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Fucntional Analysis of Sec61beta, a Component of the Sec61 protein Translocation Channel at the Endoplasmic Reticulum
… der M-Phase des Zellzyklus wird Sec61b durch die cdc2-Kinase phosphoryliert. Die Phosphorylierung erfolgt sowohl beim humanen als auch beim Drosophila Protein an einem stark konservierten Serin innerhalb eines cdc2-Konsensus Motivs. Die Mutation dieser Position kann die Lethalität des sec61bP1 …
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Sulforaphane Inhibits Mammary Cancer Cell Mitotic Progression and Tubulin Polymerization
… resulted in G2/M cell cycle arrest, elevated p34 cdc2 (cdc2) kinase activity, Bcl-2 downregulation, evidence of caspase activation, and aggregation of condensed nuclear chromatin. Subsequent exposure of synchronized cells to 15 muM SUL resulted in elevated numbers of prophase/prometaphase mitotic …
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Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis
… activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle progression dependent on cell size, thereby contributing to cell …
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Investigating the regulation of the PCTAIRE-1 protein kinase : a new target of AMP-activated protein kinase and beyond?
PCTK1 is a Cdc2-related protein kinase that is poorly characterised. Several studies have investigated its role and regulation, and have provided some insight into its upstream regulation by PKA/CDK5 phosphorylation. Proposed physiological functions include vesicle trafficking and neurite …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
… brief, phosphorylation of the Emi2 N-terminus by Cdc2/cyclin B targets it for proteasomal degradation in meiosis I (MI). During anaphase of MI, the APC/C triggers its own inactivation by degrading cyclin B, therefore stabilizing its inhibitor, Emi2. The timely inactivation of APC/C activity …
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Targeting Myeloid Protein Kinase C Signaling to Overcome Immune Suppression and Improve Immunotherapy in Cancer
… of cross-presenting cDC1 at the expense of cDC2 and plasmacytoid DCs (pDC). Lastly, combination of PKC agonism with agonistic CD40 mAb, a form of innate immunotherapy, resulted in a reduction in tumor growth with a significant increase in intratumoral activated CD8+ T cells in a syngeneic …
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Pyp3 Involvement in Mitotic Control and Cell Growth in Fission Yeast
… pombe, tyrosine dephosphorylation of p34cdc2 is required for mitotic initiation and this reversible tyrosine phosphorylation plays a crucial role in the regulation of the cell cycle. The kinases wee1+, mik1+ and the phosphatases cdc25+, pyp3+ play opposite roles in the regulation of …
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IMMUNOLOGICAL CHARACTERIZATION OF SEVERE INFERTILE MEN REVEALED ALTERATIONS OF THE LYMPHOID AND MYELOID COMPARTMENTS
… A significant higher rate of pro-inflammatory cDC2 was observed in OAT participants compared to fertile controls. Of the cytokines analyzed, we observed an overall upregulation of myeloid-derived pro-inflammatory cytokines and of IL-10 in the seminal fluid of infertile compared to fertile men. …
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GENETIC MANIPULATION OF STORAGE ROOT DEVELOPMENT IN HORTICULTURAL CROPS
A novel cdc2-like protein kinase gene (Bvcrkl) has been isolated from sugar beet in the Norman Borlaug Institute laboratories. The expression of this gene is associated with dividing tissue, particularly the secondary vascular cambial rings of the sugar beet storage root. This gene is of interest …
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EGFR Signaling Is Necessary for Gonadotropin-Induced Steroidogenesis and Oocyte Maturation
… G protein signaling, as well as MAPK and CDK1/cdc2 intracellular signaling cascades. Pharmacologic studies using receptor antagonists as well as transgenic mouse models suggested that steroid-induced maturation is mediated through classical steroid receptors. Subsequent studies focused on …
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Molecular Mechanism of Aurora-A Kinase in Human Oncogenesis
… CNTD2 expression induces Aurora-A and cdc2 kinase activity, G2/M progression, and malignant transformation. These data indicate that CNTD2 is an oncogene and could play a pivotal role in Aurora-A activation during the cell cycle and that disruption of CNTD2-Aurora-A axis may represent a …
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