Global ETD Search
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Showing 1 to 15 of 15 for “"Cdc14"”.
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CHARACTERIZATION OF MITOTIC REGULATORS ACM1 AND CDC14
Mitotic exit and cytokinesis are driven by a complex set of processes that serve to
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REGULATION OF CHROMOSOME SEGREGATION BY CONSERVED PHOSPHATASE CDC14 AND KINASE CDC5
… from mitosis require the protein phosphatase Cdc14 whose activation relies on two consecutive protein pathways, the FEAR network and the MEN. As the polo-like kinase Cdc5 is a component of both pathways its activity is essential to Cdc14 release and in its absence Cdc14 is never released. By …
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Characterization of the specificity and functions of the protein phosphatase Cdc14
… other disease states. The protein phosphatase Cdc14 has been implicated in the general reversal of cyclin dependent kinase (Cdk) phosphorylation at the end of cell division. However, the molecular mechanisms responsible for ordered Cdk substrate dephosphorylation are poorly understood. The …
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Regulation and functions of Cdc14 in mitotic exit in Saccharomyces cerevisiae
In order to ensure the accurate formation of two daughter cells from one parental cell, the series of events that comprise the mitotic cell division cycle must be carefully regulated. Much of this regulation affects the transitions between the stages of the cell cycle, which are controlled by …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
<p>In budding yeast, the phosphatase Cdc14 is released from nucleolus to promote mitotic exit (ME). Cdc14 release and ME is controlled by mitotic cyclin-Cdk oscillation, the FEAR network including a non-proteolytic function of separase (Esp1), and the Mitotic Exit Network (MEN) indirectly activated …
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The regulation of chromosome segregation by Aurora kinase, protein phosphatase 1 and nucleolar protein UTp7
… this complex during anaphase is regulated by the Cdc14 protein phosphatase, which is kept inactive in the nucleolus until anaphase onset. I show here that the predominantly nucleolar ribosome biogenesis protein Utp7 is also present at kinetochores and is required for normal organization of …
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Role of Ame1, a central component of the Saccharomyces cerevisiae kinetochore, in kinetochore function and structure
… of spindle poles occurs in absence of Esp1 and Cdc14 activity. Following experimental evidence suggests that the spindle defect in ame1-2 is not due to the above mentioned causes, but rather results from an alternative function of the kinetochore that is compromised in the mutant in a cell cycle …
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Regulation of mitotic exit in Saccharomyces cerevisiae
… of anaphase, the MEN promotes further release of Cdc14 and maintains the phosphatase in its released state. The FEAR network is comprised of the separase Espl, the polo-kinase Cdc5, the kinetochore protein Slk19, the replication fork block protein Fobl, and the small nuclear proteins Spol2 and …
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Mitotic Exit: Thresholds and Targets
… studies from our lab showing that the release of Cdc14 phosphatase also oscillates under these conditions, the reentry of Swi5 may support a model that a kinase/phosphatase balance allows cell cycle progression in these cells. All this suggests that Swi5 promotes the transcription of genes …
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Temporal organization of the budding yeast cell cycle: general principles and detailed simulations
… put on mechanisms responsible for the release of Cdc14 from the RENT complex, Cdc14 being a major player in exit from mitosis. Simulations of the wild type strain and more than 100 mutants showed phenotypes in accordance with experimental observations. Some mutants defective in the Start and …
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Small Phosphomonoesters as Probes of Protein-Tyrosine Phosphatase Active Sites
… though at a lower rate than IphP. Cdc14 displayed little activity towards N-(cyclohexane carboxyl)-O-phospho-L-serine. The active site of VHR was mapped and amino acid residues potentially involved in binding N-(cyclohexane carboxyl)-O-phospho-L-serine were identified. The amino …
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Identification of novel regulators of mitotic exit in Saccharomyces cerevisiae
… networks, the mitotic exit network (MEN) and the Cdc14 early anaphase release (FEAR) network. In this budding yeast, coordination of nuclear migration and mitotic exit is critical to prevent aneuploidy. A surveillance mechanism known as the spindle position checkpoint ensures that exit from …
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Mechanisms underlying spatial control of exit from mitosis
… In contradiction to these results, I find that Cdc14 release is responsible for the loss of cMT-budneck interactions that precede inappropriate exit from mitosis. Lastly, through the generation of cells with two nuclei, I show that the coupling of spindle position to exit from mitosis is …