Global ETD Search
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Showing 1 to 9 of 9 for “"Ccr4-Not"”.
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Mechanisms of mRNA substrate-selection by the Ccr4-Not deadenylase complex
… by a conserved multi-protein complex called Ccr4-Not. While the poly(A) tail is a feature of almost all mRNAs, cells control the rate at which each undergoes decay by the precise targeting of Ccr4-Not in both a gene-dependent and a context-dependent fashion. Substrate-selective deadenylation …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… study of the elongation process.</p><p>The CCR4-NOT complex has been shown to have role in transcriptional initiation, elongation, and mRNA degradation with the primary role of CCR4 and CAF1 in the deadenylation and degradation of mRNA. Previous work in our lab has identified a physical …
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The enzymatic function of the CCR4 protein
<p>The CCR4-NOT complex from Saccharomyces cerevisiae, which exists in two forms in vivo: 1.9 and 1.0 x 10 6 MDa in size, is a general transcriptional regulatory complex. It regulates mRNA transcription in initiation, elongation and degradation.</p><p>The 1.0 MDa complex was purified to near …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… conserved multiprotein complexes: Pan2-Pan3 and Ccr4-Not. This thesis focuses on the in vitro characterisation of Pan2-Pan3. While Pan2-Pan3 and Ccr4-Not show poly(A) preference, it was unknown how the conserved exonucleases Pan2 and Caf1 recognise poly(A). In Chapter 2, structural and …
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Precise Temporal Regulation of Ribonucleoprotein Complexes during the Drosophila Maternal-to-Zygotic Transition
… at 31B (ME31B) and Cup, as well as AGO1 and the CCR4-NOT deadenylase to regulate the translation and stability of hundreds of maternal mRNAs, and this regulation is required for proper development through the MZT. However, regulation of maternal proteins, including these RBPs is not well-studied. …
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Identification of suppressors of spt10 that affect ADH2 expression in the yeast Saccharomyces cerevisiae
… of an spt10 mutation on ADH2 expression is a phenotype shared by a small number of genes involved in the regulation of gene expression. All six known suppressors of spt10 are either components of the CCR4-NOT Complex (CCR4, CAF1, NOT4), or have been shown to interact with the complex ( DBF2, …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
… process. I have found that yeast PAB1 does not play a simple, obstructionist role in regulating CCR4 deadenylation. Instead, PAB1-PAB1 protein interactions, as mediated by the PAB1 proline-rich region (P domain) and the RRM1 domain, are required for the CCR4 deadenylase activity. The P and …
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Functional analysis of the CAF1 protein
<p>The CAF1 protein is a component of the CCR4-NOT deadenylase complex. While yeast CAF1 displays deadenylase activity, this activity is not required for its function in vivo, and CCR4 is the primary deadenylase in the complex. In order to identify CAF1-specific functional regions required for …
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Defining Determinants of Primary Drug Resistance in Precision Cancer Therapies
… CRISPR/Cas9 screen, we identified the CCR4-NOT complex as a major determinant of sensitivity to the combination therapy whose loss renders 4E-BP1 unresponsive to drug-induced dephosphorylation, rescuing MYC translational suppression and stability. Thus, by revealing a long debated EGFR …