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Showing 1 to 20 of 21 for “"Cauliflower mosaic virus"”.
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A serological study of some cauliflower mosaic virus isolates
… assay (ELISA) was used successfully to detect cauliflower mosaic virus (CaMV) in crude leaf extracts. Small serological differences between CaMV isolates could be shown by ELISA and serum cross-absorption. Serological reactivity of CaMV was found to depend on the proteolytic degradation state …
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Effect of Liposome Encapsulation of Cauliflower Mosaic Virus and Its Dna on Plant Infectivity and Culture of Turnip (Brassica Rapa L.) Protoplasts, Their Uptake and Expression of Exogenous Dna
… the effect of liposome encapsulation of cauliflower mosaic virus (CaMV) and its DNA. When grown at temperatures below 24 C, inoculated half-leaves of turnip (Brassica rapa L. cv. Just Right) produced distinct lesions. Decolorizing and staining leaves for starch increased 2 to 3 times the …
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Structure and expression of a U1 snRNA gene from Arabidopsis thaliana
… unique to the U1a gene. Two others resemble the Cauliflower Mosaic Virus 35S box II sequence and the AT box, which is found in light-regulated genes.
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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum
… either the "super" promoter or the dual enhanced cauliflower mosaic virus 35S promoter. Eighteen transgenic tobacco plants were generated that were transformed with the above recombinant genes. Preprorelaxin, mRNA was detected in 12 of the transgenic plants. Fully processed relaxin protein was not …
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Secretion of active recombinant phytase from stably transformed soybean cells
… under the control of either a constitutive cauliflower mosaic virus 35S promoter or a soybean seed specific β-conglycinin promoter, each with or without a patatin endoplasmic reticulum (ER) signal sequence. All four vectors were sequenced and introduced into 'Williams 82' suspension culture …
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An investigation into the high level production of proteins in tobacco using transgenic plants or viral vectors
… expression vector from the RNA 3 of cucumber mosaic virus strain Y (CMV Y). The 5'- and 3'-untranslated regions (UTRs) of this genome segment were reverse transcribed, cloned and sequenced. The chloramphenicol acetyl transferase gene (CAT) was inserted between the two UTRs. This artificial …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… are placed under the control of the 35S CaMV (cauliflower mosaic virus) promoter and a NOS terminator. A plant catalase intron is inserted into the GUS reporter gene. The presence of GUS enzymatic activity, as a result of Agrobacterium-mediated transformation, was indicated by a GUS …
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Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
… synthetic core promoters that rival the viral Cauliflower Mosaic Virus 35S core promoter in activity. Finally, I use plant STARR-seq to dissect the activity of three known light-responsive enhancers. I perform deep mutational scans of all three enhancers and identify regions in which mutations …
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Heterologous Expression of Arabidopsis Thaliana Purple Acid Phosphatase Gene (Atpap15) in Crops for Phytoremediation of Sites Contaminated with Excess Phosphorus
… was overexpressed in Nicotiana tabaccum under cauliflower mosaic virus (CaMV35S) constitutive promoter. After PCR confirmation of the gene, plants were transferred to the greenhouse and allowed to grow in pots. The pots contained Sta-Green potting mix (Lowe’s Inc., Mooresville, North …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… isolate of the PAV strain of Barley yellow dwarf virus (BYDV-PAV-IL), driven by Cauliflower mosaic virus-35S promoter (CaMV-35S), were constructed to study the mechanism of replication and transcription of BYDV-PAV-IL in oat protoplasts. Linearized wild-type and mutant cDNA clones express the …
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Expression of Nanobodies in Marchantia polymorpha chloroplasts
… locations of protein products. I found that the cauliflower mosaic virus 35S promoter with dual enhancer (<sub>pro</sub>35Sx2) and cytoplasmic localisation of the protein product gave the highest yield of recombinant protein: up to ~60µg/g fresh weight (~2% of total soluble protein). However, the …
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The roles of proteinases and proteinase inhibitors in plant-nematode interactions
… the late infection stage. The suitability of cauliflower mosaic virus (CaMV) 35S promoter used for transgene constructs was evaluated in this study. It was found that the expression of proteinase inhibitors, driven by the CaMV 35S promoter, decreased in root tissues of transgenic plants during …
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Structure and Function Analysis of a Plant Transcription Activator
<p>The cauliflower mosaic virus 35S promoter is composed of several cis-elements, each of which confers a different expression pattern in transgenic tobacco. One such cis-element, activation sequence (as)-1 (-83 to -63) mediates preferential expression in root. The element contains a tandem repeat …
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DNA-protein interactions throughout the Arabidopsis calmodulin-3 gene
… activity of similar sequence elements in the cauliflower mosaic virus 35S promoter, the TGAC-repeat domain of the calmodulin-3 promoter specifically activated reporter protein expression in roots. The TGAC-repeat domain was recognized by a family of at least six TGA-like factors in cauliflower …
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Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… creeping bentgrass protoplasts. The cauliflower mosaic virus (CaMV) 35S promoter was least active; whereas the rice actin 1 gene 5’ sequence was most active among the six sequences tested. Ranked in order of activity (high to low), the other four 5’ sequences were: 1) the CaMV 35S …
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Transformation of Brassica napus cv. Westar with the beet western yellows virus coat protein gene
… DNA (cDNA) copies of the beet western yellows virus (BWYV) coat protein (CP) gene were synthesized from genomic BVVYV RNA using reverse transcriptase (RT) followed by the polymerase chain reaction (PCR). The ds cDNA copy of the BWYV CP was then cloned into plasmid pRT103, a plant expression …
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Relações coordenadas entre ciclo TCA e ciclo celular em células específicas em resposta ao alumínio em Arabidopsis thaliana
… e com promotores individuais (i) Cauliflower mosaic virus 35S (p35S:FLAG-RPL18), quase constitutivo; (ii) plastid endopeptidase (pPEP:FLAG-RPL18), específico do córtex radicular das zonas de alongamento e maturação; e (iii) SCARECROW (pSCR:FLAG-RPL18), específico do centro …
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Characterization of grapevine vein clearing virus expression strategy and development of caulimovirus infectious clones
Grapevine Vein Clearing Virus (GVCV) is a newly discovered DNA virus in grapevine that is closely associated with grapevine vein clearing syndrome observed in vineyards in Missouri and surrounding states. However, Koch's postulates have never been completed. Four chapters discussing four projects …
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Isolation and characterization of an RNA polymerase III encoded gene of Pinus radiata and its use in pine transformation
Several promoters such as the cauliflower mosaic virus 35S promoter (CaMV 35S) and its enhanced version, pEMU, the maize ubiquitin promoter, the alcohol dehydrogenase promoter (Adh) and rice actin promoter (Act1) are currently used in Pinus radiata (pine) transformation. These heterologous …
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