Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Cathepsin L"”.
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Functional Characterization of a Cathepsin L in Drosophila Melanogaster
… focused on understanding the role of a Cathepsin L like cysteine protease (CP1) in the development of dorsal ASP and wing development of Drosophila melanogaster. To analyze the function of CP1, we overexpressed and knocked down CP1, respectively, using UAS-GAL4 system in combination with …
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Inhibitors of human cathepsin L and cruzain as therapeutic agents.
Increased human cathepsin L activity is linked to invasive and metastatic cancers where it promotes degradation of the extracellular matrix. This major cysteine protease found in cell lysosomes and secreted from tissues, also plays a role in the pathology of degenerative cartilage and neurological …
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Untersuchungen zur Pathogenese der dilatativen Kardiomyopathie in Cathepsin L-defizienten Mäusen
… die defizient für die lysosomale Cysteinprotease Cathepsin L sind, prägen in einem Alter von 12 Monaten eine DCM aus (Stypmann et al., 2002). Ziel der vorliegenden Arbeit ist, die Pathogenese der Kardiomyopathie der Cathepsin L-defizienten (ctsl-/-) Maus zu untersuchen. <br>Bereits im Alter von 3 …
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Veränderungen in Proteom und Metabolismus bei dilatativer Kardiomyopathie Cathepsin L defizienter Mäuse
… Aspekten derdilatativen Kardiomyopathie bei Cathepsin L defizienten Mäusen konnten in dieserArbeit weiterführende Erkenntnisse über die Erkrankung gefunden werden. CTSLdefiziente Mäuse zeigen mit durchschnittlich mit 28,4g ein um 20% reduziertesKörpergewicht im Gegensatz zu gleich alten …
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Synthese und Eigenschaften N-Acylierter Aziridin-2,3-dicarboxylate als selektive, peptidomimetische Inhibitoren von Cystein-Proteasen der Cathepsin-L-Subfamilie
… und deren Testung an den Proteasen Cathepsin B (human), Cathepsin L (Paramecium tetraurelia), Falcipain-2 (Plasmodium falciparum) und Rhodesain (Trypanosoma brucei rhodesiense). Die Verbindungen sind als irreversible Inhibitoren der Proteasen konzipiert. Der Aziridin-Baustein als …
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Investigation of the ubiquitin-specific protease UBP41 and of the lysosomal cysteine proteases cathepsin-L and cathepsin-B as potential mediators of proapoptotic signalling
… involvement of the lysosomal cysteine proteases cathepsin-L and cathepsin-B in apoptosis pathways induced by distinct death stimuli, in particular by the tumor necrosis factor a (TNF-a). Therefore, both projects examine a possible regulation of apoptosis induction by proteases that are part of …
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The synthesis, structural characterization and biological evaluation of potential chemotherapeutic agents
Cathepsin proteases have been identified in many parasitic organisms and are involved in roles as diverse as tissue and skin penetration, virulence and immune invasion. Involvement in these key functions renders them potential targets at which to direct novel chemotherapeutic agents. The inhibitor …
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The role of digestive proteases in the rotation-resistant phenotype of the western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
… on continuous soybean diet. In addition, cathepsin L protease activity in RR-WCR populations was significantly higher than that of WT populations fed on either corn silks or soybeans. Gene expression analysis of cysteine proteases in WCR guts supports these findings; the expression level …
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Design, synthesis, and biological evaluation of dihydronaphthalene and chalcone-based anticancer agents inspired by the natural product combretastatin A-4.
… were prepared to investigate selectivity of cathepsin L mediated cleavage of thiosemicarbazone and dipeptide drug-linker conjugates. The synthesis of dipeptide linkers along with thiosemicarbazone intermediates has contributed to our preliminary investigation of cathepsin L selectivity. …
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Controlled metabolic cell labeling and bioorthogonal click chemistry for cancer targeting
… quinone 1 (NQO1), and histone deacetylase (HDAC)/cathepsin L (CTSL)). Among them, HDAC/CTSL-responsive acetylated azidomannose (DCL-AAM) mediated excellent cancer-specific labeling in vitro and in vivo, which enhanced tumor accumulation of dibenzocyclooctyne (DBCO)-drug conjugates via efficient …
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The immunomodulatory properties of helminth-derived products on dendritic cell maturation and function
… F. hepatica secreted molecules, the protease cathepsin L (rFhCL1) and anti-oxidant, sigma class glutathione S-transferase (rFhGST-si), to examine their interactions with DCs. Despite enzymatic and functional differences between these antigens they both induced IL-6, IL-12p40 and chemokine …
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Characterization of Palmitic Acid Induced Lipotoxicity in Schwann Cells
… and caspase 3/7 activation. The release of cathepsin L and B were also observed with PA treatments. However, in hyperglycemic SCs, PA-LTx induced- ER stress followed mitochondrial injury, oxidative stress, caspase 3/7 activation with a subsequent xviii lysosomal release of cathepsins. Of …
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The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
The cysteine proteinases cathepsins B, L, and S are lysosomal enzymes responsible for the degradation of endocytosed proteins. Their presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- …
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Novel Roles for A-Type Lamins in Maintaining Genomic Stability
… led us to the discovery of a cysteine protease, cathepsin L, as a novel regulator of 53BP1 and the retinoblastoma tumor suppressor proteins. These findings are significant, because they reveal unexpected functions of A-type lamins and novel pathways that affect genomic stability. Our findings …
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Structure and dynamics of Pseudomonas aeruginosa ICP
… exhibits higher affinity toward the mammalian CP cathepsin L than cathepsins H and B. Homology modelling of the PA-ICP-cathspin L interaction based on the crystal structure of the chgasin-cathpsin L complex shows that PA-ICP may inhibit the peptidases by blocking the enzyme’s active site and that …
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Identification of Cathepsin B and L as Novel Uva Targets Upstream of Cutaneous Lysosomal-Autophagic Dysregulation
… the thiol-dependent cysteine-proteases cathepsin B and cathepsin L as novel UVA-targets undergoing photo-oxidative inactivation upstream of autophagic-lysosomal dysfunction. In human skin fibroblasts, exposure to noncytotoxic doses of chronic UVA (9.9 J/cm ², twice a week, 3 weeks) …
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A New Pathway Responsible For 53BP1 Loss in Breast Cancer and Laminopathies
… levels - degradation by the cysteine protease cathepsin L: CTSL). The main questions addressed in this dissertation are whether CTSL-mediated degradation of 53BP1 is a mechanism contributing to BRCA1-mutated and TNBC tumor progression and resistance to therapy and if activation of this pathway …
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Fasciola hepatica cystatins: recombinant production, biochemical characterisation and efficacy as vaccines
… inhibitors of the major F. hepatica secreted Cathepsin L peptidases (Ki <1 nM – 6 nM) and of human cysteine peptidases L, K, S and B (Ki <1 nM – 6 nM). Immunoblot analysis showed the presence of FhStf-1, FhStf-2 and FhStf-3 in the excretion/secretion (ES) so all three inhibitors could be …
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Involvement of the lysosomal cysteine peptidase cathepsin B in tumour progression and formation of metastases
… the role of the lysosomal cysteine peptidase cathepsin B (CTSB) in tumour progression and metastasis, transgenic mice FVB/N-TgN(MMTVPyVT)634Mul, developing multifocal metastasising mammary carcinomas, were utilised. CTSB deficient mice, with a mixed C57BL/6J and 129SvJ background were made …
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Cistatinas de cebada: proteínas de defensa contra artrópodos
… assays against commercial cysteine proteases: cathepsin B-, L-, H-like and legumain have demonstrated a differencial and specific inhibitory capacity within the family of barley cystatins. The defence function has been studied analysing the effects of the 13 cystatins against different species …
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