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Showing 1 to 20 of 23 for “"Catharanthus roseus"”.
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Substrate analogs to investigate alkaloid biosynthesis in Catharanthus roseus
… directed biosynthesis of alkaloid analogs in Catharanthus roseus hairy roots and seedlings. All terpene indole alkaloids are derived from tryptamine and secologanin, which are coupled to form strictosidine by the enzyme strictosidine synthase (STR). Analysis of the tryptamine substrate …
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Gene discovery in Catharanthus roseus using virus induced gene silencing
… alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents in the treatment of a variety of cancers, however the complete biosynthetic pathway for these metabolites remains to be elucidated. …
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Reprogramming alkaloid biosynthesis in Catharanthus roseus : synthetic biology in plants
The medicinal plant Madagascar periwinkle (Catharanthus roseus) produces over 130 monoterpene indole alkaloid (MIA) natural products. Many of these compounds have pharmaceutical value, such as the anticancer agents vinblastine and vincristine. Unnatural modifications can impart novel bioactivity to …
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Functional Characterization of Monoterpenoid Indole Alkaloid (MIA) Biosynthetic Genes in Catharanthus roseus
… alkaloids (MIAs) of Madagascar periwinkle (Catharanthus roseus) are known to be among the most important source of natural drugs used in various cancer chemotherapies. MIAs are derived by combining the iridoid secologanin with tryptamine to form the central precursor strictosidine that is …
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Bioreactor design for scaleup of Catharanthus roseus hairy root cultures for production of indole alkaloids
C. roseus is the source of the important anticancer indole alkaloids, vincristine and vinblastine. Hairy root cultures are an attractive alternative for the production of plant derived secondary metabolites. C. roseus hairy root cultures were established in our laboratory by A. rhizogenes mediated …
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Metabolic, biochemical and molecular profiling of Catharanthus roseus flower cultivars and transformed hairy roots for monoterpenoid indole alkaloid accumulation /
… engineering ofvindoline bios}l1thesis in Catharanthus rosellS hairy root cultures have been performed by expressing the last step in vindoline biosynthesis [Dcacetylvindoline-4-0- acetyltransferase (DAT)]. Enzymatic profiling studies with transformed hairy roots have confirmed that …
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Biosynthesis and antibacterial activity of silver and gold nanoparticles from the leaf and callus extracts of Amaranthus dubius, Gunnera perpensa, Ceratotheca triloba and Catharanthus roseus
Submitted in complete fulfilment for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2013.
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A Catharanthus roseus mutant with trace levels of secologanin and monoterpenoid indole alkaloids does not express BIS1/BIS2 transcription factors and fails to activate iridoid biosynthesis.
The Madagascar periwinkle (Catharanthus roseus) is the sole source of the monoterpenoid indole alkaloids (MIAs) that result in several essential anti-cancer chemotherapies as well as being an important source for other MIA derived pharmaceutical agents. Most of the alkaloid and pre-alkaloid iridoid …
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Toxicology and herb-drug interaction of selected anti-hypertension plants used by lay persons in Northern KwaZulu-Natal (South Africa)
… The aqueous extracts which were toxic were Catharanthus roseus and Citrus limon and these two extracts were further tested at varied concentrations to determine dosage. The results showed that as the concentration increases, the mortality percent also increased. For the extracts tested for …
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Exploiting alkaloid biosynthesis in Madagascar periwinkle to obtain natural product derivatives and new biocatalysts
… For example, vinblastine and vincristine from Catharanthus roseus monoterpene indole alkaloid biosynthesis, and camptothecin derivatives from Ophiorrhiza pumila quinoline alkaloid biosynthesis, are anticancer agents currently used in the clinic. Strictosidine synthase is a key enzyme in the …
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Preliminary characterization of VmTPT2, a putative monoterpene indole alkaloid (MIA) transporter from Vinca minor L. (Apocynaceae)
… in epidermal cells to the leaf surface of Catharanthus roseus. Bioinformatic analysis of transcriptomes from several geographically distant MIA-producing species led to the identification of proteins with high amino acid sequence identity to CrTPT2. Molecular cloning of a similar …
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The Breakdown of Plant Cell Biomass by Fungi
… of plant cell substrates (methanol extracted), Catharanthus roseus, Daucus carota, re-autoclaved C. roseus, re-autoclaved D. carota) which preliminary studies had indicated contained the necessary nutrients for fungal growth. Incubated in a suitable manner all three fungal species were able to …
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The screening of medicinal plants traditionally used to treat diarrhoea, in Ongoye area, KwaZulu Natal.
… Baccharoides adoensis, Calfilepis laureola, Catharanthus roseus, Chenopodium ambrosioids, Chromolaena odarata, Dichrostachys cinerca, Faurea macnaughton, Hewittia mafambaricaa, Hypoxis hemerocalfidea, Ihlaza, Lippia javanica, Maytenus heterophylla, Melia azedarach, Psidium guajava, Schotia …
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Discovery, characterization, and rational design of the enzymes involved in monoterpene indole alkaloid biosynthesis in Madagascar periwinkle
… insecticides, dyes, perfumes, and biofuels. In Catharanthus roseus, approximately 130 structurally complex monoterpene indole alkaloids are produced, including the clinically used anti-mitotic drugs, vinblastine and vincristine. The common intermediate to all monoterpene indole alkaloids is …
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Understanding and manipulating alkaloid biosynthesis
… alkaloids such as vinblastine (isolated from Catharanthus roseus) and morphine (isolated from Papaver somniferum) are prescribed to treat various cancers and relieve pain, respectively. Despite this storied use and palpable presence in the current pharmacopeia, relatively little is known about …
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The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
… Probing the transcriptome assemblies of Catharanthus roseus (L.)G.Don, Vinca minor L., Rauwolfia serpentine (L.)Benth ex Kurz, Tabernaemontana elegans, and Amsonia hubrichtii, with the nucleotide sequence of the N-methyltransferase involved in vindoline biosynthesis, revealed eight new …
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Localization of monoterpenoid indole alkaloid (MIA) biosynthesis by in situ hybridization (ISH)
… plant family including the most notable Catharanthus roseus. These compounds have demonstrated activity as successful drugs for treating various cancers, neurological disorders and cardiovascular conditions. Due to the low yields of these compounds and high pharmacological value, their …
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Purification and substrate specificity of new C. roseus enzymes
… thesis I developed strategies to isolate new C. roseus enzymes and to make novel analogs of the anti-hypertensive agent ajmalicine and the anti-neoplastic agent isositsirikine. The NADPH-dependent reductases that produce ajmalicine and isositsirikine have not been isolated. To produce ajmalicine …
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Molecular characterization of Rhizoctonia spp. isolates and sustainable approaches to control Rhizoctonia diseases in ornamental nursery
… Thevetia peruviana Bignonia sp., Carissa spp., Catharanthus roseus, Eugenia sp., Quercus ilex, Phyllirea angustifolia and Pittosporum tobira. The high frequency and disease incidence detected through surveys in ornamental nurseries located in Sicily clearly show as this fungus represents a …
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A potential energy-saving heat treatment for re-circulated irrigation water and its biological mechanisms
… assay data to the real world using annual vinca (Catharanthus roseus) and P. nicotianae as a model system. The results of these studies indicated that the water temperature required to eliminate Phytophthora and bacterial species can be lowered to 48"C from 95"C if treatment time extends to 24 h. …
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