Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Carrier proteins"”.
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Study of Nitrogenase Cofactors bound to Nitrogenase Carrier Proteins
… that the L-cluster binds to the nitrogenase carrier protein NifX through His³⁵ residue. In addition, we extended the use of both site-selective labeling strategy and NifX as a protein platform to conduct a comparative electronic structure study of two nitrogenase catalytic cofactors (FeMo-co …
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Nucleocytoplasmic Localization of MAPKs
… nucleocytoplasmic localization events require carrier proteins known as karyopherins (importins and exportins), ERK2 enters the nucleus of permeabilized cells even if these carrier proteins are missing. This is explained by direct binding to proteins in the nuclear pore complex (NPC). Similar …
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Development of a technique for measuring insulin-like growth factor-I in swine:application to the study of the IN VIVO and IN VITRO effects of growth hormone in neonatal pigs
… Ultrafiltration quantitatively separated carrier proteins and IGF-I. Failure to separate these species interfered with the RIA. Using this assay, swine chronically treated with pGH had 2.6-fold higher sera levels of IGF-I than controls, whereas, serum IGF-I from a hypophysectomized animal …
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Examination of auxin transport and root development in the scd1-1 mutant in Arabidopsis thaliana
… in plant cells via asymmetrically localized carrier proteins. This polarity is believed to be established and maintained by vesicular delivery and endosomal cycling of these proteins. The SCD1 (stomatal cytokinesis defective) protein has a domain structure that suggests it may function in Rab …
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Structural studies of rebeccamycin, staurosporine, and violacein biosynthetic enzymes
… that this enzyme shares a fold with lipoprotein carrier proteins. A series of site-directed mutagenesis experiments on residues around a PEG molecule bound in the VioE structure have revealed the likely location of the active site in VioE.
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Purification of Native and Recombinant NPC1
The Niemann-Pick, Type C1 protein (NPC1) is required for the transport of lipoproteinderived cholesterol from lysosomes to endoplasmic reticulum. The 1278-amino acid, polytopic membrane protein has not been purified, and its mechanism of action is unknown. We encountered NPC1 in a search for a …
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In Pursuit of a Molecular Fountain of Youth: The Identification and Characterization of Lifespan Regulators in Drosophila
Over the past century, average human lifespan has experienced steady increase despite lack of substantial intervention or understanding of the aging process. In fact, many organisms have the latent potential to live much longer than they normally do. This indicates lifespan determination is an …
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Investigation of the mechanism of transfer of a-tocopherol by the human a-tocopherol transfer protein (H-a-TTP) /
The human a-tocopherol transfer protein (h-a-TTP) is understood to be the entity responsible for the specific retention of a-tocopherol (a-toc) in human tissues over all other forms of vitamin E obtained from the diet. a-Tocopherol is the most biologically active form of vitamin E, and to date has …
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Development of a mucosal vaccine delivery system
… methods in order to provide suitable immunogen carriers for delivery to mucosal surfaces. The nanoparticles were physically characterised by determination of size, zeta potential, and morphological appearance using a variety of analytical tools. A new method for the analysis of the chemical …
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Superantigens as vaccine delivery vehicles for the generation of cellular immune responses
… conventional antigens but instead bind as intact proteins to MHC class II molecules expressed on the surface of professional antigen presenting cells. On the hypothesis that the unique properties of superantigens may serve useful for vaccine delivery, several bacterial superantigens were …
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New Approaches to the Development of Peptoid Vaccines
… not immunogenic, the attachment of peptoids to carrier proteins using a linker elicited antibodies against the peptoid/linker. Such T-cell dependent antigens elicited high-affinity, class-switched antibodies. The goal of this dissertation research was to continue optimizing the magnetic and …
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Entwicklung eines Biosensors zum Nachweis von Antibiotika und Sulfonamiden in Milch- Herstellung der immunchemischen Komponenten
… was prepared by linking haptens to carrier-proteins. After optimizing test sensitivity and characterizing the test specificity indirect EIAs could be developed for each antimicrobial compound, fulfilling the MRL requirements due to EU regulation 2377/90. Only for ampicillin and …
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Biochemical Characterization of Niemann-Pick C: A Disease of Cholesterol Transport
… where cholesterol is liberated from plasma lipoproteins that have entered the cell through receptor-mediated endocytosis. This cholesterol is transported from the lysosome to other cellular membranes to perform structural and regulatory roles. A clue to the mechanism of this cholesterol movement …
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The Hydrophobic Handoff Between NPC2 and the N-Terminal Domain of NPC1 in the Export of Cholesterol from Lysosomes
Low density lipoproteins (LDL) and related plasma lipoproteins deliver cholesterol to cells by receptor-mediated endocytosis. The lipoprotein is degraded in late endosomes and lysosomes where its cholesterol is released. Egress of cholesterol from late endosomes and lysosomes (hereafter referred to …
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Computational Evaluation and Structure-based Design for Potentiation of Nicotine Vaccines
… complex (MHC) class II molecules. The proteins were subsequently expressed in E. coli and purified using a multi-step process. Comparisons between the separation outcomes revealed that the insert was able to modulate individual process outcomes and improve overall yield without …
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Factors that Affect the Immunogenicity of Lipid-PLGA Nanoparticle-Based Nanovaccines against Nicotine Addiction
… size, hapten density, hapten localization, carrier protein, and molecular adjuvants, on its immunogenicity has been investigated. Results indicated that all these studied factors significantly affected the immunological efficacy of the nicotine nanovaccine. First, 100 nm nanovaccine was …
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Discovery and Characterization of the Polycomb Repressive Complex 1 of C. Elegans
… MIG-32 a RING protein similar to some Polycomb proteins that were identified in a yeast two hybrid screen with UNC-3 as bait. The Polycomb repression complex 2 (PRC2) methylates histone H3 lysine 27 at target genes to modify gene expression, and this mark is recognized by PRC1, which …
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The Impact of Pleiotrophin on Breast Cancer Progression
Page xiv is misnumbered as page xv.
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Visualizing Synaptic Specificity with GRASP
… to the extracellular portions of transmembrane carrier proteins in apposing cells. When complementary CD4-tethered GFP fragments were brought into proximity at sites of cell contact, GFP fluorescence was observed both in vitro and in vivo. Split GFP fragments fused to the presynaptic phosphatase …
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