Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Carboxyfluorescein"”.
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THE DESIGN OF A NOVEL ANTI-TUMOUR DRUG DELIVERY SYSTEM USING IMMUNE-DERIVED CELLS.
… prepared containing either the fluorescent probe carboxyfluorescein located in the liposomal aqueous phase, or the anticancer drugs doxorubicin or mitoxantrone which located in the lipid bilayer. Unelicited murine peritoneal macrophages were chosen as the mcrophage model for the present study …
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Insulin based analogs HLVEALYLV and LVEALYLV to inhibit the formation of amyloid fibrils in beta cells
… synthesized and tested in the presence of hIAPP. Carboxyfluorescein-encapsulating vesicles that mimic the $-cell were created using a 7:3 ratio of the lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3- (phospho-L-serine) (DOPS), respectively, and the percent …
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Eine molekulare Fliegenfalle zur Erkennung von biologisch relevanten (poly)-anionischen Substraten
… Rezeptor konnte zusätzlich ein Sensorsystem mit Carboxyfluorescein auf Basis eines indicator displacement assays entwickelt werden, mit dem die Anwesenheit von Citrat im Gegensatz zu anderen Carboxylaten mit dem bloßen Auge (naked eye) erkannt werden kann. Neben den Polycarboxylaten zeigt der …
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Peptide nanovesicles: supramolecular assembly of branched amphiphilic peptides
… concentrations of the fluorescent dye, 5(6)-Carboxyfluorescein can be encapsulated in these assemblies and delivered into human lens epithelial cells and MCF-7 cells grown on coverslips. Besides fluorescent dyes, we have delivered the plasmid (EGFP-N3, 4.7kb) into N/N 1003A lens epithelial …
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Chlorpyrifos and chlorpyrifos oxon induce neurite retraction and cytoskeletal disruption in mouse N2a cells and human neural progenitor stem cells
… cells, as indicated by morphometric analysis of carboxyfluorescein succinimidyl ester-labelled cells. Retraction of neurites was observed within 2 h of exposure by live cell imaging. Neurofilament disruption was detected in treated cells, by indirect immunofluorescence with anti-phosphorylated …
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The design, synthesis and evaluation of selective, non invasive imaging agents for atherosclerotic plaque.
… sensitive bond in between the donor (5(6)-carboxyfluorescein) and quencher (aminoanthraquinone) moiety. Legumain mediated activation of the FRET substrates was measured via fluorescence spectroscopic analysis which showed the release in fluorescence that was initially quenched by the …
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Biostabilization of Lipid Bilayers: Dealing with Water Stress in Embryos of Artemia franciscana
… desiccation-induced damage were evaluated by carboxyfluorescein leakage. AfrLEA2 (cytoplasmic) and AfrLEA3m (mitochondrial) were able to offset damage during drying of liposomes that mimicked the lipid compositions of the inner mitochondrial membrane, outer mitochondrial membrane, and the …
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Interleukin-15 and [alpha](1,3)galactosyl-expressing vaccine combination therapy for melanoma
… mice with pulmonary B16 melanomas. In vivo 5,6-carboxyfluorescein diacetate, succinimidyl ester (CFSE) labeling experiments demonstrated that mice receiving the combination therapy developed CD8 + T cells which proliferated to a greater degree than CD8+ T cells from mice vaccinated with …
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Investigating the function of the multidrug transporter ABCG2
… that ABCG2 effluxes ethidium and identified carboxyfluorescein succinimidyl ester (CFSE) as another novel substrate. Experiments with proteoliposomes containing purified inside-out oriented ABCG2 revealed that an interior alkaline ΔpH was necessary and sufficient for ABCG2-mediated ethidium …
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Synthesis of mannosylated peptides as components for synthetic vaccines
… in biological screens, a fluorescent label (5(6)-carboxyfluorescein) was introduced into the glycopeptides via the Nα- or the Nε-amino group of the lysine residue. It was found that preparation of the glycopeptide was more facile when the peptide chain was built onto the Nε of Lys (label into Nα) …
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Studies of multicomponent assemblies
… of AT-PAMAM dendrimers and an indicator, carboxyfluorescein, to detect and identify various polyanions at a low micromolar concentration. The system was able to successfully differentiate twelve anions, many of biological interest, including three tricarboxylates. The tricarboxylates were …
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Hybrid Cyclic-Linear Cell-Penetrating Peptides Containing Alternative Positive and Hydrophobic Residues as Molecular Transporters
… (F′-FTC), Stavudine (F′-d4T)), where F′ is carboxyfluorescein, were measured in the presence of the peptides in CCRF-CEM and SK-OV-3 cells. Among all peptides, [(RW)<sub>5</sub>K](RW)<sub>5</sub> (10 μM) was the most efficient transporter and improved the cellular uptake of F′-GpYEEI (2 μM) …
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Vaccination and immunological memory
… was unrelated to their cognate one. For this, carboxyfluorescein diacetate succunimidyl ester (CFSE)-labeled OT-II cells were adoptively transferred, either as naïve or 4 following in vitro activation with OVAp, into C57/BL6 wild type recipient mice which were immune to TT. Recipient mice were …
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Monogeneans of the Southern Fiddler Ray, Trygonorrhina Fasciata (Rhinobatidae) in South Australia: an exceptional model to compare parasite life history traits, invasion strategies and host specificity.
… Experiments using the fluorescent dye, 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE) revealed B. octohamatus on gills of T. fasciata within 30 min of exposure to the host. This provides strong evidence that larvae invade the gills directly via the host’s inhalant respiratory …
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Impact of environmental factors on viability and stability and high pressure pretreatment on stress tolerance of Lactobacillus rhamnosus GG (ATCC 53103) during spray drying
… wurde. Dafür wurde die Farbstoffkombination Carboxyfluorescein-diacetat und Propidiumiodid angewandt. Es zeigt sich, dass während bei der Hitzebehandlung oberhalb 60°C der Verlust der Membranintegrität als primäre Ursache des hitzebedingten Zelltodes ausgemacht werden konnte, führte …
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Proteinkonjugationen durch Kupfer-abhängige Formylglycin-generierende Enzyme zur Generierung von Antikörper-Wirkstoff-Konjugaten
… loaded with monomethyl auri-statin E (MMAE) and carboxyfluorescein. In the cell-based methods live cell imaging and ala-marBlue® assay a direct correlation between the presentation of the EGFR on the cell surface and the ADC-dependent fluorescence or cytotoxicity was demonstrated, respectively. …
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Induction of death in myeloid leukaemia cells by doxorubicin and betulinic acid, singly and in combination was associated with the regulation of apoptosis, autophagy and the PI3K/Akt pathways
… viable cells). Proliferation rate (using 5(6)- carboxyfluorescein diacetate succinimidyl ester) and cell death population (using Annexin V/propidium iodide) of the treatments were studied using flow cytometry. The contribution of reactive oxygen species (ROS) stimulation in MOLM-13 cell death …
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Dissection of phloem transport in cucurbitaceae by metabolomic analysis
… to be functional, as indicated by phloem-mobile carboxyfluorescein tracer studies. Commissural sieve tubes interconnect phloem bundles into a complete super-symplastic network. 6) Extrafascicular phloem represents the main source of exudates following physical incision. The major transported …
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Engineering Resistance to <i>Orobanche</i> <i>aegyptiaca</i>: Evidence of Sarcotoxin IA as an Anti-Parasite Protein and Macromolecule Movement From Host to Parasite
<i>Orobanche</i> species are parasitic weeds that subsist on the roots of many dicotyledonous plants. These parasites form symplastic and apoplastic connections with their hosts and act as strong sinks for the uptake of water, minerals, and photosynthates, often causing severe damage to the hosts. …
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THE ROLE OF EXTRACELLULAR VESICLES IN HEART DISEASES
… (FACS). FACS analysis included the use of (6)-carboxyfluorescein diacetate N-succinimidyl ester staining to gate intact EVs and immunophenotyping for surface markers (e.g. CD9, CD63 for tetraspanins; CD14 for monocytes; CD206 for macrophages; CD202b for endothelial cells; CD172a for …