Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Capture Hi-C"”.
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Investigation of transcription factor binding at distal regulatory elements
… remote regulatory elements, termed enhancers, which are brought into contact with promoters via DNA looping. Enhancers typically contain clusters of transcription factor binding sites, and TF recruitment to them is thought to play a key role in transcriptional control. In this thesis I have …
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The interplay of global chromosomal organisation, promoter-enhancer interactions and transcription
All somatic cells within an organism contain the same genetic material, yet they display pronounced differences in function and morphology. Precise control of gene expression is of fundamental importance to allow cells to properly develop, maintain homeostasis, and respond to external stimuli. The …
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UNCOVERING STRUCTURE-FUNCTION RELATIONSHIPS IN CHROMATIN ARCHITECTURE
… such as gene expression and DNA replication, which are crucial to establishment and maintenance of cell identity in human development. Recent technological advances have enabled the creation of high-resolution maps of chromatin architecture genome-wide from which to test new biological …
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Identificazione e analisi funzionale di fattori regolatori dei geni globinici
… we applied “Genome Wide Chromosome Conformation Capture” (Hi-C), followed by a novel technique for a selective enrichment at these target regions, to characterize whether they are involved in long range physical interactions able to modulate HBS1L-MYB and BCL11A expression. As a first step we …
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Integrative statistical methods for the genomic analysis of immune-mediated disease
… Chapters 2 and 3, I hypothesised that promoter-capture Hi-C (PCHi-C) data might have utility in physically linking disease-associated regulatory variants to their target genes, in a tissue-specific manner. To examine the genome-wide enrichment of GWAS summary statistics within PCHi-C chromatin …
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Genome-Wide and Multi-Scale Mapping of DNA Supercoiling in Differentiating Human Stem Cells
… levels to accommodate the two-meter-long DNA within the confined nuclear space of six microns. Chromatin folding is not only a cellular need, but is also crucial for gene regulation, and coordinates the precise rewiring of gene expression programmes in every cell type during developmental …
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Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
To study the interplay between spatial nuclear architecture and transcriptional activity during viral infections, I employed a genome-wide chromosome conformation capture approach (Hi-C) on infected murine and human cells and further enriched those libraries for genomic loci of interest and the …
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Investigating the role of rare genetic variants in the aetiology of haemostasis disorders
High throughput sequencing and publicly accessible genomic resources increased the diagnostic yields for inherited conditions, and, nowadays, the genetic bases for thousands of Mendelian disorders have been identified. However, providing a molecular diagnosis for these conditions remains …
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Investigation of the three dimensional organisation of the genome in B cell development
… diverse recombination events. To understand how this is achieved a capture Hi-C (CHi-C) approach was developed to investigate interactions within the Ig loci at high resolution. Hi-C libraries were enriched for the Ig loci and other genes of interest, using a biotinylated RNA bait system. In this …
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STUDIES OF B CELL DEVELOPMENT AND V(D)J RECOMBINATION
… separation of the recombination process, in which DH-JH precedes VH-DHJH recombination. Successful recombination of both heavy and light chain loci results in the expression of an antigen receptor on the cell surface. Subsequent selection stages remove non‑functional and autoreactivity …
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Genome organization by loop extrusion
… Despite their importance, the mechanism by which SMC complexes compact chromosomes remains unsolved to this day. This thesis presents a detailed analysis of the recent "loop extrusion" hypothesis of the SMC action. This hypothesis postulates that, upon binding to chromosomes, SMCs …