Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"Capsids"”.
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Computational studies on biological membranes and viral capsids
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms
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Model of a proposed local interaction mechanism leading to symmetric global structure in virus capsids
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.
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Increased Apoptosis in the Host By Guanidine Hydrochloride Treatment of Infectious Pancreatic Necrosis Virus
… apoptosis within their host. Particles or empty capsids that produce inefficient or no protein products will allow host cells to undergo apoptosis. Empty capsids are mass produced by some viruses or can be created from wild type virus by nucleic acid extrusion. This process has been done with …
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Multi-modal imaging of herpesvirus infection
… a complex assembly pathway in the cell. Capsids assemble in the nucleus, cross the nuclear envelope into the cytoplasm by budding and fusion through the nuclear membranes, and acquire a proteinaceous ‘tegument’ layer in the cytoplasm before becoming enveloped at trans-Golgi network …
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Systems Engineering for Viral Vector Manufacturing
… has low yields and low fraction of full capsids in harvest. The low percentage of full capsids in harvest (1) leads to high doses which could trigger immunogenecity reaction in patient and (2) requires extensive purification which leads to increased development product cost. In order to …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… is urgently needed. Meanwhile, AAV empty capsids as virus-like particles (VLP) produced in mammalian cells have raised increasing interest for vaccine development and drug delivery. This thesis established a new approach to produce AAV VLPs using E. coli and in vitro capsid formation. The …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
… (EM) analysis revealed that DNA-containing capsids as well as A- and B-capsids were present in the nuclei of both ΔUL25MO- and KUL25NS-infected cells. As expected, none of the progeny from ΔUL25MO-infected Vero cells expressing the wt pUL25 formed plaques on non-permissive cells. Of the 17 …
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Statistical Mechanical Models Of Virus Capsid Assembly
… in the last decade. Advances in imaging of virus capsids-the protective protein shells-in a wide variety of stages of assembly have encouraged physical assembly models at a similarly wide variety of scales, while the apparent simplicity of the capsid system-typically, many identical units …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
… genomes and their packaging into preformed capsids. Several lines of evidence have suggested that UL33, along with the UL15 and UL28 gene products, forms part of a terminase enzyme responsible for catalysing this process. This thesis describes the creation and characterisation of a number of …
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Statistical thermodynamics of virus assembly
… is studied. We show that conical and cylindrical capsids have similar energy at high surface tension of the viral membrane, which leads to the various shapes of HIV-1 capsids. Secondly, the problem of RNA genome packaging inside spherical viruses is studied using RNA condensation theory. For weak …
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Engineering minimally immunogenic cargos and delivery modalities for gene therapy
… has identified endogenous proteins that can form capsids and package nucleic acid. In this work, I focus on the PNMA or Paraneoplastic MA-containing protein family to engineer a delivery system that can form capsids, package nucleic acid, and deliver functional, minimally immunogenic cargo to …
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PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
… by detergent and salt and was associated with capsids, features common to inner tegument proteins. ICP0 mutations that resulted in the absence of ICP0 in the tegument layer, allow herpes simplex virus to enter cells independently of the proteasome activity. I propose that proteasomal …
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Differential modulation of HIV-1 capsid uncoating kinetics revealed by a novel single-particle uncoating assay
… real-time uncoating kinetics of authentic HIV capsids in vitro. This novel assay allows us to visualise the dynamic interactions of molecules with hundreds of individual capsids and determine their effect on capsid stability. We found that spontaneous uncoating is initiated shortly after the …
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Role of the Small Terminase Subunit Encoded by Staphylococcus Aureus Pathogenicity Island SaPI1 in Formation of SaPI1 Transducing Particles
… redirects packaging of its DNA into SaPI1-sized capsids.
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MUTAGENESIS OF AAV CAPSID PROTEINS FOR ENHANCED TRANSDUCTION OF CARDIAC CELL TYPES
… surface domains of AAV9 and chimeric AAV9-2 capsids. Mutations in the VP3 capsid sequences, cloned into helper plasmids pXR9 and chimeric pXR9-2 (substituting the 3-fold loop region of AAV9's VP3 with AAV2's), were used to generate a small library of rAAV9 or chimeric 9-2 vectors with mutant …
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A Role for Mindbomb 1 in Adenovirus Genome Delivery
… genomes into the nucleus for replication. Viral capsids strategically navigate this vast distance (and all subsequent lifecycle steps) through the coordinated hijacking of a number of cellular proteins subsequently termed host factors. Given the virus' dependence on these proteins, host factors …
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Glycoprotein M and ESCRT in herpes simplex virus type 1 assembly
… requires the membrane wrapping of tegumented capsids in the cytoplasm, a process termed secondary envelopment. This process involves the coordination of numerous viral and cellular proteins and results in double-membrane structures with enveloped virions contained within cellular vesicles. …
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Investigating the structure and function of HSV-1 tegument proteins: UL7 and UL51
… occurs in the nucleus, after which, DNA-loaded capsids enter the cytoplasm where they undergo tegumentation and assembly. This thesis presents a structural and functional investigation of two HSV-1 tegument proteins, UL7 and UL51 that are conserved across all herpesviruses. Deletion of UL7 or …
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Protein and viral partitioning in two-phase aqueous micellar systems
… between the C10E4 micelles and the tailspikes/capsids of bacteriophage P22 (model virus). These possible attractive interactions were investigated because they could counter the effect of the excluded-volume interactions. However, the attractive interactions between the bacteriophage P22 …
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Immune responses to AAV gene therapy in the ocular compartment
… cells. Mechanistically, it was shown that the capsids demonstrated reduced binding affinity to the AAV2 primary receptor, heparan sulphate proteoglycan, and as such it was suggested that reduced sequestration of virions in the heparan-rich inner limiting membrane may increase cellular infection …
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