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Showing 1 to 20 of 203 for “"Capsid"”.
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Statistical Mechanical Models Of Virus Capsid Assembly
… in the last decade. Advances in imaging of virus capsids-the protective protein shells-in a wide variety of stages of assembly have encouraged physical assembly models at a similarly wide variety of scales, while the apparent simplicity of the capsid system-typically, many identical units …
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The Multifunctional Capsid Gene of Hepatitis C Virus
… Hepatitis C Virus displays great diversity. The capsid gene, however, is remarkably conserved and this has led to predictions of RNA secondary structure and an overlapping, alternative reading frame (ARF). We investigated a role of the ARF in HCV infection, by introducing four stop codons into …
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Capsid catalysis : de novo enzymes on viral proteins
Biocatalysis has grown rapidly in recent decades as a solution to the evolving demands of industrial chemical processes. Mounting environmental pressures and shifting supply chains underscore the need for novel chemical activities, while rapid biotechnological progress has greatly increased the …
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Genetic and Biochemical Analyses of the Flaviviridae Capsid Proteins
… particles, thought to arise by budding of the capsid protein and associated genomic RNA through a host cell-derived lipid membrane outfitted with envelope glycoproteins. The process of virion morphogenesis is not well understood, but the presumed involvement of numerous viral and cellular …
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Investigation into the mechanism of immature HIV-1 capsid assembly
… resulting in formation of a<br/>spherical capsid immediately below the host cell membrane. The resulting immature, noninfectious<br/>virions are released from the cell and the viral protease, PR, hydrolyses Gag into<br/>its component domains. MA remains at the membrane and NC remains in …
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HIV needs the small molecule IP6 to build its capsid
… Immunodeficiency Syndrome (AIDS). The viral capsid protein (CA) is essential for replication. Upon budding of a new HIV particle, CA is part of the Gag polyprotein which assembles into an immature virus lattice. During maturation, the viral protease cleaves Gag into single components and CA …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… is urgently needed. Meanwhile, AAV empty capsids as virus-like particles (VLP) produced in mammalian cells have raised increasing interest for vaccine development and drug delivery. This thesis established a new approach to produce AAV VLPs using E. coli and in vitro capsid formation. The …
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Secretion of the rotavirus VP7 capsid glycoprotein from polarised epithelial cells
… rotavirus, the VP7 glycoprotein forms the outer capsid layer of the virion where it is a key target for neutralising antibodies, which act to prevent virion decapsidation. Both polarised and non-polarised cell culture models have been widely used in rotavirus research. However, cell …
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Functions of scaffolding protein in phage P22 capsid assembly and maturation
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Design of a large-scale virus capsid model for educational use
A foot-scale model of an icosahedral virus capsid, as opposed to a helical capsid shape, was modeled in preparation for manufacture for educational use. We chose to model the icosahedral virus capsid for manufacture because understanding the structure of this type of capsid is of biological …
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MUTAGENESIS OF AAV CAPSID PROTEINS FOR ENHANCED TRANSDUCTION OF CARDIAC CELL TYPES
… surface domains of AAV9 and chimeric AAV9-2 capsids. Mutations in the VP3 capsid sequences, cloned into helper plasmids pXR9 and chimeric pXR9-2 (substituting the 3-fold loop region of AAV9's VP3 with AAV2's), were used to generate a small library of rAAV9 or chimeric 9-2 vectors with mutant …
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Engineering fluorescent imaging platforms to characterise molecular interactions with HIV-1 capsid
The HIV-1 capsid is a protein shell utilised by the virus as a binding platform to hijack host machineries to promote infection. Characterisation of novel cofactors binding to capsid demands sensitive and quantitative assays to better understand their underlying binding mechanisms. Here, I have …
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Design and manufacturing of a physical model of an icosahedral virus capsid
… of the development of a model of a virus capsid for educational and illustrative purposes. This thesis begins with a study of the virus and its capsid structure. It studies the icosahedral structure and the theory of quasi-equivalence, which were favored by viruses throughout their …
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Development of an Assay to study the Kinetics of HIV-1 Capsid Uncoating
… evolve resistance to therapeutic intervention. Capsid uncoating is the process by which p24CA proteins (CA) disassemble from the viral ribonucleoprotein during the early phase of the HIV-1 lifecycle. Despite intensive investigation, much is yet unknown about the spatial and temporal occurrence …
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Modulation of West Nile Virus Capsid Protein and Viral RNA Interaction through Phosphorylation
… the interaction between WNV viral RNA and the capsid (C) protein and also elucidate how the processes of nucleocapsid assembly are regulated. Initial in vitro C protein and viral RNA interaction studies showed that this interaction was not specific. This suggested the presence of a regulatory …
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Self-assembly of the human papillomavirus capsid: light scattering studies and kinetic models
Includes bibliographical references (pages 133-135).
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HEPATITIS A VIRUS: GROWTH CHARACTERISTICS, PURIFICATION, AND CAPSID GENE ORDER (PEPTIDES, IMMUNOREACTIVITIES, POLYPEPTIDES).
… potential immunogenic regions within the HAV capsid polypeptides. Synthetic peptides corresponding to selected regions of each of the larger putative capsid polypeptides were coupled to keyhole limpet hemocyanin and used to immunize rabbits. EITB reactivities of HAV specific anti-peptide sera …
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Helper phage capsid size redirection by staphylococcal pathogenicity island SaPI1 involves internal scaffolding proteins.
… is remodeling of the normal T=7 bacteriophage capsid to produce smaller T=4 phage-like particles. These particles, composed entirely of helper phage proteins, can accommodate the smaller SaPI genome while excluding that of a complete helper phage. This study was designed to understand the …
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Structural Studies On The Rubella Virus Capsid Protein And Its Organization In The Virion
… to its alphavirus relatives. The rubella capsid protein is a critical structural component of virions as well as a key factor in virus-host interactions. Three crystal structures of the structural domain of the rubella capsid protein have been described here. The polypeptide fold of the …
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