Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Capping Protein"”.
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MOLECULAR MECHANISMS OF ACTIN FILAMENT ASSEMBLY AND TURNOVER
… primarily from the myriads of actin binding proteins (ABPs) that bind to and regulate actin. A substantial proportion of these proteins modify actin turnover at filament ends, defined as the flux between actin’s monomeric G-actin state and its filamentous F-actin state. Our understanding of …
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Java-based modeling of the actin polymerization cycle
… model that describes the effects of regulatory proteins (profilin, beta-thymosin, cofilin, and capping protein) on the steady-state actin cycle. The model necessitates the simultaneous solving of 90-330 differential equations and 51-171 additional equations. One object of this thesis was to …
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A biochemical investigation of actin disassembly mechanisms
… filaments. We showed that Aip1 is not an actin capping protein as was previously thought, however it can destabilize cofilin-saturated stable filaments and potentiate cofilin’s severing and depolymerization activity. During the course of our work we also uncovered some insights on the biophysics …
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Study of proteins after recovery from bacterial infection
… The objective of this study was to compare the proteins and peptides present in the microglia of BCG-challenged mice one-week after treatment relative to control mice and gain insights through differential detection and enrichment analysis. Detection relied on tandem mass spectrometry …
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Distinguishing Macrophage Activation States by Mass Spectrometry
… MASCOT database search suggested the macrophage-capping protein (CAPG) as source of the peptide, which could be validated through peptide sequencing through post-source decay experiments conducted on the tryptic fragments</p>
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Calcium/Calmodulin Dependent Protein Kinase Type-II Associates with Flightless-I to Influence its Nuclear Localization
Ca2+/calmodulin-dependent protein kinase type-II (CaMK-II) is a Ser/Thr protein kinase regulated by Ca2+ and Calmodulin. It is a highly conserved and broadly expressed enzyme and has a unique structure and dynamic regulation. It has the ability to remain active in the absence of Ca 2+ as a result …
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The Function of CARMIL1 in Migrating Cells
… describes the physiological role of the Capping Protein- CARMIL interaction in migrating cells. I establish the CARMIL-CP complex as a key regulator of lamellipodial actin assembly and of lamellipodial dynamics. Membrane ruffling at the leading edge of motile cells and macropinocytosis …
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Role of the immunoglobulin superfamily member Basigin in sensory neuron dendrite morphogenesis in Drosophila
… residues that may bind cytoskeletal adaptor proteins is required for its function in neurons. Results of genetic interaction analysis suggest that Basigin-mediated regulation of dendrite morphogenesis does not involve Integrin and matrix metalloproteinases, both of which have been implicated …
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Actin polymerization dynamics at the leading edge
… filaments, we tested how profilin, Arp2/3, and capping protein (CP) function together to propel beads or thin glass nanofibers coated with N-WASP WCA domains. During sustained motility, physiological concentrations of Mg²⁺ generated actin filament bundles that processively attached to the …
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The Role of Zasp52 in the Morphogenesis of the Drosophila melanogaster Embryo
… processes share unique features, such as protein composition or organisation of components of morphogenetic functions. In order to study common features and possibly functions of supracellular actomyosin cables in the fruit fly embryo, this work capitalised on prior findings that the …
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The Role of Sirtuin Inhibitors on the Proteomic Responses of the Mussels Mytilus galloprovincialis and Mytilus trossulus to Menadione Induced Oxidative Stress
… analysis utilizing 2D-gel electrophoresis and protein identification via mass spectrometry showed that 18% and 17% of all identified protein spots detected demonstrated changes in abundance in <em>M. galloprovincialis </em>and <em>M. trossulus, </em>respectively. Using matrix-assisted laser …
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Functional studies of selected actin binding proteins by point mutations and GFP fusions
Profilin is an ubiquitous cytoskeletal protein whose function is fundamental to the maintenance of normal cellular physiology. Site-directed mutagenesis of profilin II from Dictyostelium discoideum by PCR resulted in the point mutations W3N and K114E, whereby the W3N profilin is no longer able to …