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Showing 1 to 16 of 16 for “"Cap Structure"”.
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Purification, characterization and substrate specificity of a nuclear mRNA (Guanine-7)-Methyltransferase from Ehrlich ascites cells
… the sequence of m7G(5')ppp(5')N- known as the "cap" structure. The guanine-7 methylation of this cap structure is important for mRNA processing and initiation of translation. A RNA (guanine-7)-methyltransferase that specifically methylates the 5'-terminal guanosine residue of the RNA cap …
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The mRNA Cap and Discontinuous Transcription in Trypansoma brucei
… mini-exon genomic DNA, in order to analyze the structure of medRNA, the 140- nucleotide precursor of the mini-exon in mRNA. Some possible intermediates in the joining reaction and the 5' mini-exon cap structure were detected and characterized. Using this direct RNA analysis, the 5' and 3' ends …
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Biochemical and Structural Characterization of Divergent Members of the Protein Kinase Family
… Glutamylation, and NiRAN-catalyzed RNA capping. A Legionella pneumophila effector SidJ adopts a kinase fold yet catalyzes calmodulin (CaM)-dependent glutamylation to inactivate the SidE ubiquitin ligases. SidJ becomes activated upon translocation into the host cell and uses cellular ATP …
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Initiation of Translation of Eukaryotic Messenger-Rna at a Unique Site Lacking an AUG Codon
… the ribosome recognizes a 5$\sp\prime$ terminal cap structure (m$\sp7$GpppN ...) and then scans down the mRNA until reaching the first 5$\sp\prime$ terminal AUG or $\sp{\rm A}\sb{\rm G}$XXAUG$\sp{\rm A}\sb{\rm G}$. The RNA from satellite tobacco necrosis virus (STNV RNA) is unusual in that it …
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Evaluation of translation initiation features of satellite tobacco necrosis virus RNA
… in the absence of a 5$\sp\prime$-terminal cap structure and initiator AUG codon. The translational properties, in wheat germ in vitro translation system, of transcript STNV RNAs (tSTNV RNAs), with modifications to the initiator AUG codon and the 5$\sp\prime$-terminal sequences normally …
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Regulation of hnRNP A1 Cellular Localization by Protein Kinases and its Biological Impact
… recruit ribosomes independently of the 5’ m7G cap structure. hnRNP A1 (heterogeneous nuclear ribonucleoprotein A1), a multifunctional RNA binding protein, is required for the IRES-dependent translation of many specific RNAs within the cell cytoplasm. The phosphorylation of hnRNP A1 is required …
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Characterization of features of the initiation of translation of satellite tobacco necrosis virus RNA
… to initiate efficiently in the absence of a cap structure and in the absence of an AUG initiation codon. These unusual characteristics of STNV RNA were shown to reside in the 5$\sp\prime$-untranslated region by replacing these upstream sequences with either a random sequence or the …
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
<br/>The N-7 methylguanosine cap structure is conserved from yeast to man. It is essential for cell proliferation as it influences several steps in eukaryotic gene expression including transcription, pre-mRNA processing, RNA export and translation. The N-7 methylguanosine cap is added …
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Genomic Analysis of Human and Mouse Guanine-7-Methyltransferase with Active Site Characterization
… 5' end of eukaryotic and viral mRNAs contain a "cap" structure with the sequence m7G(5')pppN(5'). The methylation of the 7-position on the guanine cap is very important to proper mRNA processing and initiation of translation. The enzyme responsible for this methylation, RNA …
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Elucidating the Roles of Eukaryotic Initiation Factors Involved in DAP5 Mediated Translation
… Initiation Factor (eIF4F), which consists of the cap binding protein eIF4E, the scaffolding protein eIF4GI, and the helicase factor eIF4A.The recognition and binding of eIF4E to the m<sup>7</sup>G cap structure of the mRNA is essential for the subsequent recruitment of eIF4GI and eIF4A to form a …
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The translation initiation site of satellite tobacco necrosis virus RNA does not require a start codon
… subunit recognizes the 5$\sp\prime$ terminal cap structure (m$\sp7$G(5$\sp\prime$)ppp(5$\sp\prime$)N$\...$) and scans along the messenger RNA until an initiator AUG codon is found in a favorable context sequence ($\sp{\rm A}\sb{\rm G}$XXAUGG). Satellite Tobacco Necrosis Virus (STNV RNA) and …
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On co-transcriptional splicing and U6 snRNA biogenesis
… before it can be translated into a protein: a cap structure is added to its 5’ end, noncoding, intervening sequences (introns) are removed and coding exons are ligated together and a poly(A) tail is added to its 3’end. Splicing, the process of intron removal, is carried out in the spliceosome, …
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Regulatory consequences of mRNA Poly(A)-Tail length changes
Eukaryotic mRNAs have a cap structure at their 5' ends and a poly(A) tail at their 3' ends, and the proteins that bind these features increase the stability and translation of an mRNA. The influence of the poly(A) tail on translation was discovered decades ago, but primarily with regard to the idea …
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Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction
… initiation factor 4E (eIF4E) binds the 5’m7GTP cap structure of mRNA in order to facilitate effective translation. Recessive eIF4E alleles harboring naturally occurring point mutations have been associated with resistance to viral infection, particularly in members of the genus Potyvirus. …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
The mRNA cap consists of a series of co-transcriptional modifications on nascent transcripts that confer protection from degradation and mediate RNA processing and translation. The addition of an inverted guanosine cap to the 5’-end of the transcript and its subsequent methylation along with the …
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Differential Regulation of Iress In The Aurora A Mrna By Bfgf Through The Mtor Complex to rc2 Modulates Aurora A Kinase Expression
… The canonical pathway utilizes the m7G cap structure at the 5’ end of the mRNA to recruit the translational machinery including the 40S ribosome which scans the mRNA for the AUG start codon. Alternatively, the 40S robsome is recruited downstream of the cap to internal ribosomal entry …