Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Calpains"”.
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The activity and content of calpains in maturing dystrophic muscle membranes
Increased calcium-activated calpain proteolysis in the sarcolemma membrane is thought to be a primary mechanism in the pathophysiology of Duchenne Muscular Dystrophy (DMD). However, few studies have tested this possibility prior to the overt signs of the dystrophy. The purpose of this study was to …
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N-terminomics/proteomics investigation of Calpain-2 substrates
Calpains are a family of intracellular cysteine proteases that require calcium for activation. Calpain-1 and -2, first described as the classical calpains due to their ubiquitous expression, are part of this larger 15-member family in humans and share similar patterns of expression and structural …
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The characterisation of calpain-like proteins in Trypanosoma brucei
Calpains are a ubiquitous family of calcium-dependent cysteine proteases involved in a wide range of cell regulatory and differentiation processes. In many protozoan organisms, atypical calpains have been discovered that lack the characteristic calcium-binding penta-EF-hand motif of typical …
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Calpain inhibitor design inspired by the natural inhibitor calpastatin
Calpains are cysteine proteases that depend on Ca2+ for activation. They have many roles in the cell; hence modifications of calpains or changes in their levels of activity can lead to altered physiological states. Thus, it is of great interest and medical relevance to design inhibitors that can …
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Exploring the effect of genetic calpain disruption on cell migration, invasion, drug sensitivity, and metastasis in triple negative breast cancer
… reported to promote spread to secondary sites. Calpains are a family of calcium-activated proteases that have emerged as a potential target in triple-negative breast cancer. Calpains cleave substrates involved in various cell signalling pathways associated with tumourigenesis and metastasis. …
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Regulation of Cyclin G2 Degradation in Human Ovarian Cancer Cells
… assays, we showed that cyclin G2 is a target of calpains proteolytic activity in a number of human ovarian cancer cells. Furthermore, we found that inhibition of EGFR in those cells led to protection of cyclin G2 from degradation. Finally, we demonstrated that EGF stimulation also resulted in …
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Preclinical Evaluation of Matrix Metalloproteinase Inhibitors and Protein Kinase C Activators in Cell and Mouse Models of Huntington’s Disease
… HTT is cleaved by proteases, including caspases, calpains and matrix metalloproteinases (MMPs). Previous research has also shown altered kinase signaling pathways in HD contribute to the localization of mutant huntingtin to the nucleus and to disruption of transcriptional regulation. Currently, …
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Investigation into the lipid activation of calpain and its role in cataract formation
… is easily administered and equally effective. Calpains are Ca2+dependent intracellular proteases and several of these enzymes are believed to participate in cataractogenesis. Calpain 2 is the major isoform of calpain involved in human cataractogenesis and its activation appears to be …
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Targeting Calpain-1 and Calpain-2 for Prevention of Breast Cancer Metastasis: In Vivo Insights and Drug Discovery Approaches
… and therapeutic resistance. To evaluate calpains’ potential as therapeutic targets, we used CRISPR-Cas9 gene editing to knock out calpain-1 and/or calpain-2 in MDA-MB-231 triple negative breast cancer (TNBC) cells. Compared to WT cells, calpain knockouts exhibited a 79% reduction in in …
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Mechanisms associated with secretory and contractile responses in tissues and cells of the body in health and disease
… and anti-secretory drugs in the stomach and calpains in relation to vision. The study was designed to understand diseased states ( eg diabetes mellitus, pancreatitis, gastric ulcers, cataractogenisis and the dry eye syndrome) compared to healthy conditions. The results have demonstrated that, …
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Increased Mitochondrial Calpain-1 Is an Important Mechanism Contributing to Mitochondrial ROS Generation in Cardiac Diseases
… septic cardiomyopathy. Given that mitochondrial calpains also increase and contribute to myocardial injury in ischemic hearts, our findings suggest that increased mitochondrial calpain-1 may be a common mechanism contributing to mtROS generation and myocardial injury in the pathogenesis of …
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Non-lysosomal protein degrading systems in chicken skeletal muscle
… I species. A proportion (up to 14%) of the calpains in crude muscle extracts was bound to membrane fractions in the presence of ca2+; this could be removed by EGTA treatment. In addition, membrane-bound fractions examined by 9el filtration contained calpain· forms of an apparent molecular · …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
… from other cysteine proteases including calpains, cathepsin B and L, indicating the cysteine protease was either an uncharacterized protein or a known protein with unknown myosin specificity.
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Non-lysosomal protein degrading systems in chicken skeleton muscle
… I species. A proportion (up to 14%) of the calpains in crude muscle extracts was bound to membrane fractions in the presence of ca2+; this could be removed by EGTA treatment. In addition, membrane-bound fractions examined by 9el filtration contained calpain• forms of an apparent molecular · …
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Molekulargenetische Analyse der Central Core Disease
… stellt das Gen für die kleine Untereinheit des Calpains dar, das möglicherweise direkt in die elektromechanische Koppelung zwischen Nerv und Muskel involviert ist und mit dem Ryanodinrezeptor interagiert. CCD-Patienten wurden mit der SSCP-Methode (Single-stranded conformation polymorphism) auf …
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Leucine and exercise improve skeletal muscle function in the mdx mouse
Duchene muscular dystrophy (DMD) is a lethal X-linked disease that afflicts approximately 1 in 3500 newborn males. Boys with DMD will become progressively weaker causing wheelchair dependence by their early teens and death by their mid to late twenties. Currently there is no cure for DMD, the exact …
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Investigation of the effects of emerging technologies on the kinetics of glycolytic and proteolytic enzymes relevant for meat quality
… muscle. Enzymes involved in proteolysis include calpains and cathepsins. Carcass interventions offer the potential to modulate these biochemical processes that are key to determining meat quality. Several interventions are currently used by the meat industry, including aging (wet and dry), …
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Determining the mechanism of pulsed electric field for improving meat quality : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
Pulsed electric field (PEF) is a novel non-thermal technology that has recently attracted the attention of meat scientists and technologists due to its ability to modify membrane structure and enhance mass transfer. Several studies have confirmed the potential of PEF for improving meat tenderness …