Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 129 for “"Calcium imaging"”.
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Computational tools for monitoring neural connectivity using calcium imaging
The development of optogenetics and calcium imaging have enabled light-activated manipulation and monitoring of neural activity that offer powerful alternatives to traditional electronic and chemical methods. Along with light-evoked activity, many technical advancements have been made that allow …
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Microfluidics for Calcium Imaging of C.elegans Neurons During Temporally Precise Odor Stimulation
… thesis, I provide a protocol for high-throughput calcium imaging in C.elegans. I used the nematode worm, C.elegans as a model system for several reasons: (1) odor-evoked responses have been well characterized, both at the neural and behavioral level1, (2) scientists have mapped out all of the 302 …
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Molekulare und phänotypische Charakterisierung von Drosophila melanogaster Synapsin Mutanten und In-vivo Calcium Imaging
… Teil-Projekt konnten Mutationen in die cDNA des Calciumsensor Cameleon 2.0 Proteins eingebracht werden, um so die verbesserte Version Cam 2.1 zu erhalten. Daraufhin wurden mehrere transgene UAS-Cam 2.1 Linien hergestellt, die bei der Kreuzung mit verfügbaren Gal4 Linien den Calciumsensor für eine …
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Two-photon calcium imaging sequence Analysis Pipeline : a method for analyzing neuronal network activity
… as autism and Alzheimer's disease. Two-photon calcium imaging has been a powerful tool for the investigation of the development of neuronal networks. However, one of the major challenges of working with two-photon calcium images is processing the large data sets, which often requires manual …
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Optische Visualisierung neuronaler Aktivität : Etablierung des in-vivo Calcium-Imaging mit dem genetisch codierten Sensor Yellow Cameleon 2.1 und Untersuchung der olfaktorischen Codierung im Gehirn von Drosophila melanogaster
… vor: Zum einen wurde die Technik des in – vivo Calcium – Imagings mit Hilfe des genetisch codierten Sensors Yellow Cameleon 2.1 am Lehrstuhl komplett neu etabliert, zum anderen wurde mit der neuen Technik das Zusammenspiel der funktionellen Elemente neuronaler Systeme anhand der …
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Using machine learning for analysis of neuronal network activity
… conditions such as Rett syndrome. Two-photon calcium imaging is used to capture neuronal network activity over time. This method produces large sets of images that are typically manually analyzed by skilled neuroscientists. Because this process is both time-consuming and subject to error, …
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Optical imaging of dendritic spikes in apical oblique dendrites of layer 5 pyramidal neurons
… of voltage-gated sodium and voltage-gated calcium channels. In addition, oblique branch spikes manifest as after-depolarizing potentials (ADP) at the soma. I then experimentally verified the generation of spikes by imaging the dendritic activity of oblique branches of L5PNs from the …
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Optical imaging of dendritic spikes in apical oblique dendrites of layer 5 pyramidal neurons
… of voltage-gated sodium and voltage-gated calcium channels. In addition, oblique branch spikes manifest as after-depolarizing potentials (ADP) at the soma. I then experimentally verified the generation of spikes by imaging the dendritic activity of oblique branches of L5PNs from the …
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Chemesthesis in the Earthworm, Lumbricus terrestris: The search for TRP Channels
… behavioral and cell dissociation/calcium imaging techniques. We obtained responses from segmental nerves to tactile stimulus, 50mM AITC and pH 3.8, 4.0 and 4.2. Some cells in the epidermis displayed positive immunoreactivity for Drosophila and human TRPA1 homologs. L. terrestris …
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Minimally invasive neuromodulation using mechanically-sensitive ion channels and magnetically-actuated nanotransducers
… of mechanoreceptors and stimulation conditions. Calcium imaging results demonstrate that this method of nanotransducer targeting can elicit neuronal responses at 40mT even via endogenous ion channels, and that greater amplitudes of response can be achieved through mechanosensitive ion channel …
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The role of estradiol in astrocyte and neuron communication
… and the subsequent intracellular calcium mobilization. This intracellular calcium signal triggers the release of gliotransmitters that, activate neuronal receptors, and thereby regulate synaptic function. Most studies on the tripartite synapse have focused on classic …
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Diversity of transduction mechanisms in receptor neurons of the main olfactory epithelium in <i>Xenopus laevis</i> tadpoles
… wurden mittels der "patch-clamp" Technik und "calcium imaging" gemessen. Sowohl Aminosäuren als auch die Wirkstoffe, die den cAMP-vermittelten Transduktionsweg aktivieren, erwiesen sich als wirksame Reize. Interessanterweise reagierten etliche ORZ, die auf Aminosäuren eine Reaktion zeigten, …
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Understanding experience-dependent plasticity of cellular and network activity in the mouse primary visual cortex
… with the induction of SRP, we have used calcium indicator expressing mice to visualize cellular activity across days of SRP training. Using two-photon calcium imaging, we found that there is indeed no significant net change in the population of active neurons during presentation of the …
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Mechanisms and plasticity of cold sensitivity in peripheral neurons
… been implicated in cold sensation. Ratiometric calcium imaging and quantitative rt-PCR studies revealed that cold sensitivity in sympathetic neurons and in a significant proportion of sensory neurons is independent of TRPM8 and TRPA1 expression, suggesting the presence of a further …
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Modulation of the sensitivity of nociceptive neurones by cannabinoids and SNSR
… which leads to the sensitisation of nociceptors, calcium imaging was used to assess if the application of a synthetic cannabinoid agonist (WIN 55,212-2) would attenuate the responsiveness of nociceptors in vitro. After examining this in both a nonsensitised model and a model of sensitisation …
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Development of a mouse model for the study of osteoarthritis pain
… genetically modified mice, novel analgesics and calcium imaging.<br/>The role of specific pain receptors in OA pain were investigated in this model of OA using genetically modified mice, novel analgesics and calcium imaging. Partial medial meniscectomy has proven to be a useful tool for the …
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The Role of Mitochondria in Oxygen Sensing of the Ductus Arteriosus and Fetal Pulmonary Arteries
… and oxygen response measured with confocal calcium imaging. Human DASMC grown for 96-hours in hypoxia (2.5% oxygen) or normoxia (19.6% oxygen) were used for 3’RNA-sequencing. Silencing RNA was used to selectively knock down ETC subunits, with effects on oxygen responsiveness examined with …
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Sensorimotor transformation and information coding across cortex during perceptual decisions
… memory-guided visual decision task. Large-scale calcium imaging revealed that neurons in each area were heterogeneous and spanned all task epochs (stimulus, delay, response). However, information encoding was distinct across regions, with V1 encoding stimulus, fMC encoding choice, and PPC …
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Phenotyping for a personalised approach to CVD prevention
… Troponin measured in blood and coronary artery calcium imaging) in the management of persons with hypertension and abnormalities in diastolic blood pressure.
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Towards mapping spatial transcriptome of an entire vertebrate brain
… as a model organism for whole-brain in vivo imaging and modeling. While calcium imaging generates substantial amounts of neural activity data, the lack of molecular information for individual neurons in a purely activity readout approach limits further biological interpretation. Recent …
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