Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Calcium flux"”.
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Synthesis and Characterisation of Dual CCR7/CXCR4 Antagonists
… antagonists were synthesised and assessed by calcium flux assay. Several potential dual CCR7 and CXCR4 biaryl sulfonamide antagonists have been synthesised, these are hybrid compounds incorporating features from CCR7 antagonists of this project, and from known sulfonamide CXCR4 antagonists. …
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Synthesis and Characterisation of Dual CCR7/CXCR4 Antagonists
… antagonists were synthesised and assessed by calcium flux assay. Several potential dual CCR7 and CXCR4 biaryl sulfonamide antagonists have been synthesised, these are hybrid compounds incorporating features from CCR7 antagonists of this project, and from known sulfonamide CXCR4 antagonists. …
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The emerging roles of BAP1 and cancer stem cells in Malignant Mesothelioma
… receptor subtype 3 (IP3R3)-mediated calcium flux. Cancer Stem-like Cells (CSCs) are a small population within the tumour mass, which are responsible for tumour initiation, cancer progression and treatment failure. Increased evidence suggests that CSCs rely on mitochondria function as …
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Structural and Dynamic Proximal Proteomic Analysis of TRPV2 Ion Channel Activation
Calcium signaling is a robust, tightly regulated pathway which mediates every vital cellular function through the propagation of localized calcium flux. Transient receptor potential vanilloid type 2 (TRPV2) ion channels are calcium-permeable, tetrameric ion channels with implications in several …
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Targeting the formyl peptide receptor 1 for treatment of glioblastoma
… responses in a range of models including calcium mobilisation, cell migration, and invasion. The efficacy of FPR1 antagonist ICT12035 in vivo was assessed in a U-87 MG subcutaneous xenograft model. Results Virtual high throughput screening using a homology model of FPR1 led to the …
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Enhancement of the An1 Population by BAFF: Potential Role in Autoimmune Disease
… whether this population is truly anergic, calcium flux assays have been performed. Interestingly, these assays show a decrease in response to BCR signaling in BAFF-treated cultures compared to untreated controls. Additional studies with sorted populations of cells indicate that these An1 …
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Pathogenic effects of Mycoplasma hyopneumoniae on porcine tracheal organ cultures and neutrophils in vitro, and porcine respiratory goblet cells in vivo
… altered neutrophil function, intracellular calcium flux was examined after incubation of porcine neutrophils with or without M. hyopneumoniae. After zymosan stimulation, intracellular calcium levels were significantly increased in neutrophils incubated with M. hyopneumoniae. Enhancement of …
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Effects of Acute Nutritional Deprivation on Lymphocyte Subsets and Membrane Function in Cats
… CD8+ cells (day 7; p < 0.04) and intracellular calcium (day 4; p < 0.02) as compared to baseline. During the refeeding period increases (p < 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p < 0.07) and lymphocyte number (p < 0.004) as compared to day …
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Investigating the metabolic impact of Metformin and Berberine in combination with Venetoclax for Acute Myeloid Leukaemia treatment
… and reduced expression of the lactate efflux protein MCT4, whereas BBR+Ven increased MCT4 expression. Both combinations increased mitochondrial membrane permeability, correlating with increased cell death. Proteomic analysis of Met+Ven treated cells revealed decreased expression of core …
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Design, Synthesis and Evaluation of FPR-1 Antagonists in the Treatment of Glioma
… inhibit cell migration for the antagonists using calcium flux assays and scratch assays, respectively. MTT assays confirmed that the antagonists would be non-toxic to cells. Most novel antagonists showed improved lipophilicity, but none showed higher potency or migratory inhibition than ICT12035. …
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Design, Synthesis and Evaluation of FPR-1 Antagonists in the Treatment of Glioma
… inhibit cell migration for the antagonists using calcium flux assays and scratch assays, respectively. MTT assays confirmed that the antagonists would be non-toxic to cells. Most novel antagonists showed improved lipophilicity, but none showed higher potency or migratory inhibition than ICT12035. …
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How TCR signal strength controls CTL polarisation for target killing
… was linked with the TCR-induced intracellular calcium flux, the duration of which increases with the strength of TCR signalling. This demonstrates how the process of CTL killing can be fine-tuned by the quality of antigen.
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The regulation of metabolic gene expression in human skeletal muscle by exercise: the influence of exercise intensity and contraction frequency
… are activated by alterations in ATP turnover, calcium flux, cellular stress and the redox state in the muscle cell. The aim of this thesis was to impact the main variables influencing muscle contraction, the contraction force and frequency, during an acute bout of exercise and to investigate …
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Role of N-terminal residues of CCL19 and CCL21 in binding and activation of CCR7
… CCR7 using a number of in vitro assays such as calcium flux, trans-well (Boyden chamber), and Western blotting. We also carried out computational studies to better understand and predict the activity of these peptides. Our results demonstrate that some of these peptides are indeed capable of …
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Role of Neurogranin In The Regulation of Calcium Binding to Calmodulin
… of Neurogranin in the regulation of calcium binding to Calmodulin</strong></p> <p><strong></strong>Anuja Chandrasekar, B.S</p> <p>Advisor: M. Neal Waxham, Ph.D</p> <p>The overall goal of my project was to gain a quantitative understanding of how the interaction between two proteins …
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Calcium Signaling Dynamics in RBL Cells Under Pulsed Ligand Exposure
… have measured with high temporal resolution the calcium response of mast cells exposed to stimulus. Cells labeled with the calcium indicator dye, Fluo-4, are loaded into the microfluidic traps. By monitoring the intensity of emitted fluorescence from the excited Fluo-4, the amount of …
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Mitochondrial regulation of CD8+ T cell cytotoxicity
… of oxidative phosphorylation, mitochondrial calcium flux and mitochondrial translation. I highlight mitochondrial translation as a previously uncharacterised mitochondrial function that allows for optimal CTL cytotoxicity. Specifically, I show that both Usp30-/- CTLs and CTLs in which …
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Modulation of neutrophil degranulation by hypoxia
… 3-kinase and phospholipase C, but not calcium flux prevented augmented granule release under hypoxia In conclusion, hypoxia induces a destructive neutrophil phenotype, with increased release of multiple histotoxic proteases. This may contribute to tissue injury and disease pathogenesis …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
… entrapment by relying on the cell’s natural calcium flux. A widely used CPP, TAT, is covalently linked to a calmodulin protein, creating TAT-CaM. The protein of interest that acts as a cargo, is engineered to have a calmodulin binding site (CBS). This allows TAT-CaM to bind cargo proteins …
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Phosphodiesterase 1 (PDE1) at the Crossroads of Calcium and Cyclic Nucleotide Signaling in Diabetic Nephropathy
… Our investigation focused on the role of calcium/calmodulin activated PDE1 in DKD. Three isoforms of PDE1 are differentially expressed in vascular smooth muscle cells, renal tubular epithelial cells, podocytes, and mesangial cells. We used highly potent and selective PDE1 inhibitor LY1 to …
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