Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Calcium Current"”.
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Calcium Channel Gamma(6) Subunits Are Unique Modulators of Low Voltage-Activated Calcium Current
The calcium channel gamma (gamma) subunit family consists of eight members whose functions include modulation of high voltage-activated (HVA) calcium currents in skeletal muscle and neurons, and regulation of AMPA receptor targeting. Cardiac myocytes express three gamma subunits, gamma 4, gamma6 …
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Expression and Characterization of Calcium Channel Alpha1 Subunits in Atrial Tissue
… tissue expresses two types of voltage-activated calcium current, a high voltage-activated (HVA) L-type and a low-voltage activated (LVA) T-type. The LVA calcium current is often associated with growth and nuclear division. In atrial tissue, the LVA calcium current demonstrates developmental …
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The Calcium Channel Gamma6 Subunit Analysis of Function and Determination of a Sequence Motif Critical for Its Effect
The auxiliary gamma6 subunit of voltage dependent calcium channels is known to decrease calcium current density when co-expressed with the pore forming Cav3.1 subunit. In this study, I showed that Cav3.1 calcium channels are a major pathway for calcium influx at resting membrane potential, and that …
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Calcium Influx via the T-Type Calcium Channel Plays a Permissive Role in Proliferation of Mouse Embryonic Hl-1 Cells
… myocytes express two types of voltage operated calcium currents, a high voltage activated (HVA) L-type, and a low voltage activated (LVA) T-type. Influx of calcium into the cell through the L-type channel is responsible for excitation-contraction coupling in the heart. The T-type calcium current …
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A quantitative study of neuronal calcium signaling
… by transmembrane voltage changes, while calcium levels within the cell communicate information on a much slower time scale. Calcium acts as a second messenger responsible for modulating neuronal excitability in many ways including the mediation of gene transcription in the cell and the …
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Glutamate Excitotoxicty Activates a Novel Calcium Permeable Ion Channel in Cultured Hippocampal Neurons
… receptors leads to protracted intracellular calcium elevations triggering calcium mediated neurotoxic mechanisms culminating in delayed neuronal cell death. In addition, glutamate excitotoxicity induces a NMDA dependent extended neuronal depolarization mediated by continuous calcium influx …
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Modeling and Ion Mapping of Intracellular Calcium Distribution in Molluscan Neural Somata
… constants of 1-3 sec) buffering of intracellular calcium in molluscan cell bodies, a diffusion-reaction model was developed. The model took into account the binding reactions of Ca('2+) with a calcium-indicating dye (arsenazo III) and an endogenous Ca('2+) buffer. The results from this model show …
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Sensory Transduction in Enteroendocrine Cells
… hormone release. In KRJ-I cells two outward currents were observed. In STC-1 cells three types of ionic currents were identified; a large outward voltage gated potassium current, a tetrodotoxin-sensitive voltage gated sodium current and a nifedipine-sensitive persistent calcium …
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Molecular Interactions and Physiological Function of the Voltage-Dependent Calcium Channel Gamma6 Subunit
… I, I give an overview of the Voltage-dependent Calcium Channel (VDCC) and its importance as a key regulator of intracellular calcium. I attempt to provide a thorough explanation of the nomenclature system used to describe the various VDCC subunits, and discuss the structure and interaction of …
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Ethosuximide stabilizes sleep-related oscillations and reduces sleep fragmentation during alcohol withdrawal
… with increased risk of relapse. Furthermore, the current first-line therapy for alcohol withdrawal, chlordiazepoxide, is inappropriate for long-term management of AWI due to potential for abuse and negative side effects. While much of the research into the mechanisms underlying AWI has focused on …
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Mathematical modelling of pulsatility in neuroendocrine systems.
… cells and methods of recording selected ionic currents are the subject of chapter three. Chapter four presents a discussion of oscillatory behaviour in nerve cells at a general level and outlines the features necessary for a nerve cell to exhibit oscillation. The next three chapters discuss the …
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Intrinsic Properties and Neuromodulator Regulation of Neuronal Excitability of Intralaminar Thalamic Neurons
… from activation of the transient, low threshold calcium current, was present in all bushy neurons; however, burst discharge was absent in the majority of diffuse neurons. Diffuse neurons primarily project to the basal ganglia, whereas bushy neurons predominantly project to frontal cortex. My …
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Characterization Of High-Voltage-Activated Calcium Channels In Retinal Bipolar Cells
… I investigated the high-voltage-activated (HVA) calcium current in retinal bipolar cells in mice. In the first part of my dissertation, I characterized multiple bipolar cell-expressing GFP and/or Cre transgenic mouse lines. In the second part of my dissertation, by performing whole-cell …
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Development and Mechanism of Oxygen Sensitivity in Caudal Hypothalamic Neurons
… frequency that was due to a sustained inward current. Interestingly, this inward current response was no different in magnitude between neurons from immature and mature rats. Pharmacological analyses demonstrated that this inward current response was due to sodium and not calcium current. This …
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T-type calcium channels and human mesangial cell proliferation
… mesangioproliferative renal diseases. The T-type calcium channel (T-CaCN) has been proposed to play an important role in the proliferation of a number of non-excitable cell types, but T-CaCN expression and functional significance in MC is not known. The aim of this thesis was to investigate the …
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The electrophysiological effects of Endothelin-1 in human atrial myocytes
… (ET-1), each of which affects the atrial L-type calcium current (ICaL) and consequently action potentials. Hypotheses: ET-1 modulates the effects of isoproterenol (ISO) on ICaL and action potentials in human atrial myocytes. Methods: Atrial myocytes were isolated enzymatically from samples of …
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DOES CALCIUM INFLUX THROUGH T-TYPE CALCIUM CHANNEL INDUCE CARDIOMYOCYTE PROLIFERATION?
… study is to investigate on how TTCCs (T-type calcium channels) affect cardiomyocyte proliferation. In mice after birth, the major TTCC expressed in the heart is Cav3.1/α1G, and therefore we used Cav3.1/α1G transgenic (TG), knockout (-/-) and wild type mice respectively to define the role of …
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The electrophysiological and molecular effects of chronic beta-adrenoceptor antagonist therapy on human atrium
… involve pharmacological remodelling of atrial K+ currents and underlying ion channel subunits. This project aimed to test the hypothesis that various characteristics of human atrial K+ currents, including voltage, time and rate dependency, differ between patients treated and not treated with a …
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Effects of endotoxin-induced inflammation on glucose metabolism and contractility of the rat heart in vivo and in vitro
… performed on isolated cardiomyocytes the calcium influx through voltage-sensitive Ca2+- channels (L-type channels) was measured. It was found that an incubation of these cells in vitro with LPS (1 µg/ml) for 1-2 h reduced the calcium current by 50%. This effect was prevented by …