Global ETD Search
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Showing 1 to 11 of 11 for “"Caf1"”.
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Functional analysis of the CAF1 protein
<p>The CAF1 protein is a component of the CCR4-NOT deadenylase complex. While yeast CAF1 displays deadenylase activity, this activity is not required for its function in vivo, and CCR4 is the primary deadenylase in the complex. In order to identify CAF1-specific functional regions required for …
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Identification of suppressors of spt10 that affect ADH2 expression in the yeast Saccharomyces cerevisiae
… either components of the CCR4-NOT Complex (CCR4, CAF1, NOT4), or have been shown to interact with the complex ( DBF2, SRB9, SRB10). This indicated that spt10 suppression may be characteristic of CCR4-like gene regulation. In this work, I conducted a screen for additional suppressors of spt10 at …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… degradation with the primary role of CCR4 and CAF1 in the deadenylation and degradation of mRNA. Previous work in our lab has identified a physical connection between the CCR4-NOT complex and SPT5. The role of SPT5 in mRNA degradation was examined. Mutation in SPT5 or an spt4 deletion slowed …
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Functions of Deadenylation Factors In Mrna Decay and Mrna Processing Body Formation
… deadenylation complexes, Pan3-Pan2 and Ccr4-Caf1, can both be enriched in P bodies. The deadenylase activity of the Ccr4-Caf1 complex is prerequisite for P body formation. Pan3, but not the deadenylase Pan2, is essential for P body formation. While the C-terminal domain of Pan3 is important …
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The function of PAB1 in translation and in PUF3 dependent deadenylation
… been shown to involve recruitment of CCR4 via CAF1, suggesting PUF proteins accelerate deadenylation by increasing the local concentration of deadenylases around the mRNA. Since PUF proteins are involved in repression of translation it has been suggested that they also accelerate deadenylation …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… unknown how the conserved exonucleases Pan2 and Caf1 recognise poly(A). In Chapter 2, structural and biochemical studies reveal that these DEDD-family deadenylases recognise the intrinsic stacked, helical structure of single-stranded poly(A) RNA. Recognition of this conformation in different …
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Remineralisierendes Potential neuartiger zahnärztlicher Lacke
… in Kombination mit Kalziumfluorid (CaF1/CaF5) eine Remineralisation der künstlichen Schmelzkaries erzielt. Ein signifikanter Unterschied zwischen der Verwendung von 5.000 ppm F- Zahnpasta zu 1.450 ppm F- Zahn¬pasta hin¬sichtlich einer Verbesserung der Remineralisationsparameter …
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The enzymatic function of the CCR4 protein
… the complex. The 1.0 MDa complex contains CCR4, CAF1, NOT1--5, and two new proteins, CAF40 and CAF130. CAF130 and CAF40 are two unique yeast proteins, with CAF40 displaying extensive homology to proteins from other eukaryotes. Immunoprecipitation and gel filtration experiments confirm that CAF130 …
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Genomic changes underlying disease resistance and high protein QTL
… genomic changes in a gamma-SNAP in cqSCN-006 and CAF1 (chromosomal assembly factor 1) in cqSCN-007 are examined for their potential to be causative for SCN resistance in cqSCN-006 and cqSCN-007. Structural variation causes high protein phenotype in cqProt-003 In addition to the G. soja genotype PI …
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Expression, purification and characterisation of recombinant chromatin assembly factor 1
Chromatin Assembly Factor 1 (CAF-1) is the only known replication dependant histone chaperone, responsible for the deposition of the histone H3/H4 tetramer onto DNA. Found in all eukaryotes, CAF-1 consists of three subunits, p150, p60 and p48. Since its identification work on CAF-1 has mainly …
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The development of Greenland's shrimp fishing and processing industry since 1979: A study in applied economics
Shrimp fishing and processing is Greenland's dominant industry, and one which is crucial to the country's economy. However, since the late 1980s it has faced a state of crisis characterised by overcapacity, poor returns and threatened stocks. Despite these facts, the literature in this area is …