Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 81 for “"Caco-2 cells"”.
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Development of in vitro Chylomicron Assay Using Caco-2 Cells
… model system. As an <em>in vitro</em> model Caco-2 cells are able to secrete lipoproteins. We investigated the possible factors that may affect the secretion of CMs through the ultracentrifugation technique. The dose-dependent effects of oleic acid, mono-olein, egg lecithin, collagen matrix, …
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In vitro starch digestibility and ex-vivo glucose response studies of Vietnamese starchy foods using Caco-2 cells and 14C radioisotope
… and ex-vivo glucose response using a Caco-2 cell model. The potential of using black rice as an alternative to white rice was also studied. Eleven rice starches were analysed for amylose content, swelling power, gelatinisation (DSC) and pasting (RVA) properties, degree of …
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The intestinal absorption of n-3 and n-6 polyunsaturated fatty acids: proposed mechanisms from studies using human Caco-2 cells.
… mechanism by which they first enter absorptive cells - to be metabolised for release in blood circulation - is still not fully elucidated. Cellular and molecular aspects of fat-absorption have been neglected since it was long time believed that fatty acids simply diffuse through the epithelium. …
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Intestinal absorption barriers as modelled by p-glycoprotein and cytochrome p450 a34 in caco2 cells
… passage number and origin of two populations of Caco-2 cells influence their enterocyte-like characteristics. Caco-2 cells of passage number >90 from Novartis pharmaceutical company possess higher levels of expression of alkaline phosphatase and P-glycoprotein and a greater cellular uptake of …
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Effects of GABA on inflammation and intestinal barrier disruption
… of EAE mice and in a monolayer composed of Caco-2 cells exposed to inflammatory mediators. Intestinal permeability of the in vivo model was measured by the oral administration of 4-kDa fluorescein isothiocyanate (FITC)-labeled dextran 19 days post-EAE induction. Results showed increased …
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Regulation of mTORC1 Signalling and Amino Acid Uptake by Iron Availability in Caco-2 Intestinal Epithelial Cells
… (DFO) was used to induce iron deficiency in Caco-2 cells (a cell line derived from a human colorectal carcinoma which has functional properties typical of absorptive enterocytes of the small intestine) in order to assess the effects of iron depletion on various aspects of the physiology of …
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… The Mouse Small Intestinal Epithelial (MSIE) cells are conditionally immortalized. It was found that MSIE cells express BoAT1, CAT1, CAT2, LAT1, y+LAT1, and y+LAT2, but not PepT1, EAAT3, Bo,+AT, or LAT2, making this model similar to the basolateral membrane of enterocytes. Growing MSIE cells …
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Cell-Specific Inhibition of Interferon Production by Rotavirus
… of two human cell lines; the intestinal (Caco-2) and the alveolar basal (A549) epithelial cells. Our findings confirmed the potential ability of the virus to establish extra-intestinal infection in A549 cells. Furthermore, our results showed up-regulated IFNs and ISGs transcription in …
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Relationship Between Flavonoid Structure And Phase-II Metabolism
… in backbone on the disposition of flavonoids in Caco-2 cells; 5) study the rate-limiting role of efflux transporters in the disposition of flavonols in Caco-2 cells; and 6) study the regiospecific disposition of flavones in Caco-2 cells. Method: For objectives 1 and 2, in vitro recombinant human …
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The role of the rho-associated coiled-coil containing kinase (ROCK) in cytokine-induced chemokine responses in intestinal epithelial cells
… chemokine expression in intestinal epithelial cells (IEC), which is mediated by Rho-associated kinase (ROCK). Because ROCK may be an important mediator of inflammation, we extended our investigations to examine the role of ROCK in TNF-α-stimulated chemokine responses in IEC. Inhibiting ROCK …
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Role of Extracellular Signal-Regulated Kinase (ERK) in Regulation of Intestinal Epithelial Tight Junctions
… ERK protects tight junctions in differentiated Caco-2 cells, while it is disruptive to tight junctions in under-differentiated cells, and that in differentiated cells, ERK regulates the activity of PKCζ to enhance Thr-phosphorylation of occludin.</p> <p><strong>Specific Aims</strong>: 1. To …
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Small Proline Rich Protein-2 Expression and Regulation in the Caco-2 model of Intestinal Epithelial Differentiation along the Crypt-Villus Axis
… in the GI tract is largely unexplored. Using the Caco-2 model of intestinal epithelial differentiation along the crypt-villus axis, we hypothesized that SPRR2 would be preferentially expressed in post-confluent differentiated Caco-2 cells and examined SPRR2 regulation by the protein kinase A …
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Effetto pro-ossidante e pro-infiammatorio degli ossisteroli in cellule intestinali: attività modulatoria della frazione fenolica del vino
… and pro-inflammatory effect of oxysterols in Caco-2 cells, a cell line with enterocyte-like features, as a model system that mimics the insult of dietary oxidized lipids on the intestinal mucosa. Oxidative damage was induced by treating cells with a dietary-representative oxysterols mixture …
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Solid Lipid Nanoparticulate Formulation for ifosfamide: Development and Characterization
… and the delivery systems were performed using Caco-2 cells. Subcellular localization studies using rhodamine loaded nanoparticles were performed using Caco-2 cells. Different chamber pressures during lyophilization produced products having different morphology and moisture content. The drug in …
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Preparation, Characterization and In Vitro Evaluation of Metformin Loaded Hyaluronic Acid Nanoparticles for Oral Delivery
… at a concentration of ≤ 100 µM in Cacco-2 cells. Haemolysis studies indicated that these nanoparticles can also be safely administered via intravenous route. Cellular uptake studies on Caco-2 cells indicated higher uptake of metformin from nanoparticle relative to that from metformin …
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Antiproliferative Activity of Carotenoids, Phenolic Compounds, and Volatile Terpenoids in Carrots (Daucus carota L.)
… volatile terpenoids extracted from carrots on Caco-2 colon cancer cells in vitro. Briefly, carrot carotenoids, phenolics, and volatiles were extracted from carrots using liquid-liquid, solid phase, and distillation extraction techniques respectively. 1000 Caco-2 cells were seeded in a 96-well …
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Lipid Signaling Under Microgravity Conditions
… by Space stressors. Intesti-nal epithelial cells (IECs), which form the intestinal lining, respond to environmental insults – including pathogens and other abiotic stressors – by interacting with the immune system and triggering inflammatory responses. Inflammation represents a …
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Effect of Soyasaponin Fractions on Human Colon Cancer Cells
… on the human colon adenocarcinoma cell line Caco-2. Experiments were conducted to determine the effects of purified soyasaponins on cell proliferation, Protein Kinase C (PKC) activity, and cell morphology in cultures of Caco-2 cells. Treatment of cells with soyasaponins I and III at …
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Novel polyelectrolyte complexes for oral insulin delivery.
… was determined by MTT assays carried out on Caco-2 cells with or without the inclusion of a 24-hour cell recovery period. An MTT assay conducted without a recovery period indicated that quaternisation of Paa was associated with a six-fold improvement in its IC50. Additionally, cells subjected …
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