Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"Caco-2 cell"”.
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Uptake and transport of orally-deliverable drugs across caco-2 cell monolayers:the effect of lipid formulations
… of the Iiposome. formulations towards the Caco-2 cell line, employed to model the epithelial barrier in vitro. Uptake and transport of mannitol, propranolol, glutamine and digoxin was measured in the presence and absence of Iiposome formulations to establish changes in absorption resulting …
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Interaction of peptides, peptidomimetics and other drug candidates with the DI/tripeptide transport system in intestinal epithelial cells, using the in vitro caco-2 cell culture system
An uptake system was developed using Caco-2 cell monolayers and the dipeptide, glycyl-[3H]L-proline, as a probe compound. Glycyl-[3H]L-proline uptake was via the di-/tripeptide transport system (DTS) and, exhibited concentration-, pH- and temperature-dependency. Dipeptides inhibited uptake of the …
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A Comparative Study of Iron and Zinc Concentration, Localization, Speciation and Bioavailability in Two Wheat Cultivars
… bioavailability of Fe in wheat using an in vitro Caco-2 cell model system. Speciation of Fe and Zn varied between milling fractions with low molecular weight (LMW) complexes likely to be Fe-deoxymugenic acid/nicotianamine and Zn-nictainamine being the predominant extractable Fe and Zn species in …
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An Investigation of The Use of Dendrimer-Based Carrier to Cross Cellular Barrier
… drug delivery system to overcome the cellular barriers. G3 PAMAM dendrimers were conjugated with lauryl chains via a carbamate bond. Paclitaxel, a low water solubility P-gp substrate and anti-tumour drug, was conjugated to G3 and/or lauryl-G3 PAMAM dendrimer conjugates via a glutaric …
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In vitro starch digestibility and ex-vivo glucose response studies of Vietnamese starchy foods using Caco-2 cells and 14C radioisotope
… and ex-vivo glucose response using a Caco-2 cell model. The potential of using black rice as an alternative to white rice was also studied. Eleven rice starches were analysed for amylose content, swelling power, gelatinisation (DSC) and pasting (RVA) properties, degree of …
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Hyperinvasiveness in the major food-borne pathogen Campylobacter jejuni
… infections. Invasion of host epithelial cells is believed to be an important virulence Mechanism of this bacterium. C. jejuni strains vary in their ability to invade the human epithelial cells and some of the strains are hyperinvasive. The aim of this work was to find the molecular basis …
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Diversified strategies for improving iron bioavailability of maize
… soy hemoglobin (LHb) for FeBA using the in vitro Caco-2 cell model compared to bovine hemoglobin (BHb), and ferrous sulfate (FeSO4). When iron sources were tested alone, the FeBA values of LHb and BHb were 19% (P > 0.05) and 113% (P < 0.001) higher than FeSO4, respectively. However, when iron …
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Nherf1 – New Modifier of Colorectal Cancer Progression
… epithelium into adenocarcinoma. Loss of cell polarity has been implicated as an early event in this process, but the molecular players involved are not well known. NHERF1 (Na+/H+ Exchanger Regulatory Factor 1) is an adaptor protein with apical membrane localization in polarized epithelia. …
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INVESTIGATING ALTERNATIVE SPLICING REPROGRAMMING REGULATED BY THE E-CADHERIN/B-CATENIN AXIS
… of the myosin VIshort isoform, promoting tumor-cell migration. Using the Caco-2 cell line, capable of modulating isoform expression under different conditions, we uncovered the molecular mechanism behind this dysregulation, pinpointing E-cadherin and β-catenin as central players in myosin VI AS …
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Strategies to improve non-haem iron absorption
… iron in an available form for uptake into Caco-2 cells (measured by ferritin formation). A human study was undertaken to assess the bioavailability of ferrous gluconate in alginate beads, and it was found to be significantly lower than ferrous gluconate on its own, so further in vitro …
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Intestinal absorption and metabolism of bush tea major phenolic compounds exhibiting anti-diabetic activity.
… in vitro when tested in muscle, liver and fat cells. While an aqueous extract of A. phylicoides has been shown to have bioactivity, there is limited knowledge regarding absorption and bioavailability of phenolic compounds present in A. phylicoides. Aim of the study: The present study aims to …
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Pre-clinical development of cyclodextrin-based nanoparticles for oral delivery of protein/peptide drugs-in vitro and in vivo evaluation
… of the formulations. In vitro studies using the Caco-2 cell monolayer model and ex vivo (rat intestinal tissue) studies showed that insulin glulisine NPs enhanced the epithelial permeability of insulin. Lastly, the formulations were assessed in different animal models. Best results were obtained …
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Experimental Approaches to Understand and Control Salmonella Infection in Poultry
… evaluated for decreasing the gut permeability in Caco-2 cell lines. The result from animal experiments indicated that a combined batch culture of 3 selected strains showed significant efficacy in controlling intestinal colonization of ST in neonatal turkey poults.</p>
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Effects of GABA on inflammation and intestinal barrier disruption
… of EAE mice and in a monolayer composed of Caco-2 cells exposed to inflammatory mediators. Intestinal permeability of the in vivo model was measured by the oral administration of 4-kDa fluorescein isothiocyanate (FITC)-labeled dextran 19 days post-EAE induction. Results showed increased …
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Solid dispersions: formulation, characterisation, permeability and genomic evaluation
… dispersions as compared to drug alone across Caco-2 cell monolayers. From the cell uptake studies it was shown that PEG 8000 enhanced rhodamine123 uptake which suggested that PEG 8000 may increase the permeability of these drugs in solid dispersions. Gene expression profiles analyzing the …
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Improved iron and zinc availability in sorghum by phytate reduction through genetic modification, fermentation and phytase addition
… using a dialysability assay (bioaccessibility), Caco-2 cell (uptake) study and an in vivo suckling rat pup model (absorption). A mineral solubility assay was used to analyse the soluble minerals in the raw cereal wort. GM low phytate (approx. 30-40% reduction) non-tannin and tannin sorghums and …
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Relationship Between Flavonoid Structure And Phase-II Metabolism
… in backbone on the disposition of flavonoids in Caco-2 cells; 5) study the rate-limiting role of efflux transporters in the disposition of flavonols in Caco-2 cells; and 6) study the regiospecific disposition of flavones in Caco-2 cells. Method: For objectives 1 and 2, in vitro recombinant human …
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Mechanism(s) involved in the transport of Fenretinide across Caco-2 monolayers
… for the transport of femetinide across Caco-2 cell monolayers with an aim to determine the possible reasons for poor oral absorption of fenretinide. Fenretinide was found to be highly lipophilic (log P = 7.4) and practically insoluble in water. The water solubility of fenretinide was …
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… The Mouse Small Intestinal Epithelial (MSIE) cells are conditionally immortalized. It was found that MSIE cells express BoAT1, CAT1, CAT2, LAT1, y+LAT1, and y+LAT2, but not PepT1, EAAT3, Bo,+AT, or LAT2, making this model similar to the basolateral membrane of enterocytes. Growing MSIE cells …
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