Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 166 for “"Caco-2"”.
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Development of in vitro Chylomicron Assay Using Caco-2 Cells
… model system. As an <em>in vitro</em> model Caco-2 cells are able to secrete lipoproteins. We investigated the possible factors that may affect the secretion of CMs through the ultracentrifugation technique. The dose-dependent effects of oleic acid, mono-olein, egg lecithin, collagen matrix, …
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Mechanism(s) involved in the transport of Fenretinide across Caco-2 monolayers
… for the transport of femetinide across Caco-2 cell monolayers with an aim to determine the possible reasons for poor oral absorption of fenretinide. Fenretinide was found to be highly lipophilic (log P = 7.4) and practically insoluble in water. The water solubility of fenretinide was …
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Einfluss des Flavonoids Quercetin auf die epitheliale Barrierefunktion der humanen Kolonkarzinom-Zelllinie Caco-2
… die Barrierefunktion der Kolonkarzinom-Zelllinie Caco-2 zu untersuchen. Hierbei zeigte sich, dass Quercetin konzentrationsabhängig (50-200 µM) den transepithelialen Widerstand erhöhte. Die Wirkung von 200 µM Quercetin war bereits nach 4 h Inkubation erkennbar und erreichte nach 48 h maximale …
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Investigations into the effects of berry flavonoids on nutrient transport processes in Caco-2 enterocytes
… expression microarray analysis on intestinal Caco-2 cells treated with a flavonoid-rich berry extract (0.125 % w/v for 16 h). This microarray analysis identified alterations in numerous specific nutrient pathways, three of which were selected for further study, namely: 1) glucose, 2) iron and …
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Uptake and transport of orally-deliverable drugs across caco-2 cell monolayers:the effect of lipid formulations
… of the Iiposome. formulations towards the Caco-2 cell line, employed to model the epithelial barrier in vitro. Uptake and transport of mannitol, propranolol, glutamine and digoxin was measured in the presence and absence of Iiposome formulations to establish changes in absorption resulting …
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Regulation of mTORC1 Signalling and Amino Acid Uptake by Iron Availability in Caco-2 Intestinal Epithelial Cells
… (DFO) was used to induce iron deficiency in Caco-2 cells (a cell line derived from a human colorectal carcinoma which has functional properties typical of absorptive enterocytes of the small intestine) in order to assess the effects of iron depletion on various aspects of the physiology of …
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In vitro starch digestibility and ex-vivo glucose response studies of Vietnamese starchy foods using Caco-2 cells and 14C radioisotope
… and ex-vivo glucose response using a Caco-2 cell model. The potential of using black rice as an alternative to white rice was also studied. Eleven rice starches were analysed for amylose content, swelling power, gelatinisation (DSC) and pasting (RVA) properties, degree of …
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The intestinal absorption of n-3 and n-6 polyunsaturated fatty acids: proposed mechanisms from studies using human Caco-2 cells.
… study, a human enterocyte cell model (Caco-2 cells) was used. A careful attention has been taken on the specific parameters that influence the transport characteristics of the Caco-2 cells and the accessibility of the fatty acids to the absorption site. The results presented in the …
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Small Proline Rich Protein-2 Expression and Regulation in the Caco-2 model of Intestinal Epithelial Differentiation along the Crypt-Villus Axis
… in the GI tract is largely unexplored. Using the Caco-2 model of intestinal epithelial differentiation along the crypt-villus axis, we hypothesized that SPRR2 would be preferentially expressed in post-confluent differentiated Caco-2 cells and examined SPRR2 regulation by the protein kinase A …
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Modulatory effect of EVOO polyphenols and their metabolites on inflammatory response in Adenocarcinoma Colon Cells (Caco-2) and Human Umbilical Vein EC (HUVEC)
… inflammatory response at intestinal level (in Caco-2 cell monolayer) and endothelial level (in HUVEC cell monolayer), using pro-inflammatory stimuli, such as LPS and/or a hyperglycemia (HG) condition. Their modulatory action was evaluated focusing on the alteration of tight junctions (TJ) and …
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Glucocorticoid receptor signalling and the effect of interleukin 1 beta on glucocorticoid mediated gene expression in intestinal epithelial cell lines Caco-2 and IEC-6
… pathway in the human colon carcinoma cell line Caco-2 and rat intestinal cell line IEC-6. The second aim of this study was to test the hypothesis that IL-1b, a major cytokine in IBD may inhibit glucocorticoid receptor action. Methods: The subcellular distribution of glucocorticoid receptor was …
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Interaction of peptides, peptidomimetics and other drug candidates with the DI/tripeptide transport system in intestinal epithelial cells, using the in vitro caco-2 cell culture system
An uptake system was developed using Caco-2 cell monolayers and the dipeptide, glycyl-[3H]L-proline, as a probe compound. Glycyl-[3H]L-proline uptake was via the di-/tripeptide transport system (DTS) and, exhibited concentration-, pH- and temperature-dependency. Dipeptides inhibited uptake of the …
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Phospholipid Transfer Protein (Pltp): Secretion, Gene Expression, and Functions in Cultured Cells
… by using a human small intestine cell model, Caco-2. Properties of Caco-2 secreted-PLTP were similar to those of purified human plasma PLTP. Treatment of the cells with butyrate and propionate enhanced PLTP secretion and PLTP gene expression.
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Intestinal absorption barriers as modelled by p-glycoprotein and cytochrome p450 a34 in caco2 cells
… passage number and origin of two populations of Caco-2 cells influence their enterocyte-like characteristics. Caco-2 cells of passage number >90 from Novartis pharmaceutical company possess higher levels of expression of alkaline phosphatase and P-glycoprotein and a greater cellular uptake of …
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Cell-Specific Inhibition of Interferon Production by Rotavirus
… of two human cell lines; the intestinal (Caco-2) and the alveolar basal (A549) epithelial cells. Our findings confirmed the potential ability of the virus to establish extra-intestinal infection in A549 cells. Furthermore, our results showed up-regulated IFNs and ISGs transcription in …
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Effects of GABA on inflammation and intestinal barrier disruption
… of EAE mice and in a monolayer composed of Caco-2 cells exposed to inflammatory mediators. Intestinal permeability of the in vivo model was measured by the oral administration of 4-kDa fluorescein isothiocyanate (FITC)-labeled dextran 19 days post-EAE induction. Results showed increased …
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Relationship Between Flavonoid Structure And Phase-II Metabolism
… in backbone on the disposition of flavonoids in Caco-2 cells; 5) study the rate-limiting role of efflux transporters in the disposition of flavonols in Caco-2 cells; and 6) study the regiospecific disposition of flavones in Caco-2 cells. Method: For objectives 1 and 2, in vitro recombinant human …
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