Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Ca2+ signals"”.
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High-resolution optical analyses of IP3-evoked Ca2+ signals
Ca2+ is a universal intracellular messenger that regulates many cellular responses. Most cells express inositol 1,4,5-trisphosphate receptors (IP3R) that mediate Ca2+ release from the endoplasmic reticulum (ER) when they bind IP3 produced after activation of cell-surface receptors. Vertebrate …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… over cellular behavior by endogenous re-wiring. Ca2+ signals allow cells to regulate diverse processes such as migration, apoptosis, motility and exocytosis. In some receptors (e.g. VEGFR2), Ca2+ signals are generated upon ligand binding (e.g. VEGF-A). Here, firstly we engineered fusion proteins …
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Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells
… the spatial and temporal changes of the free Ca2+ ion concentration ([Ca2+]) inside skeletal muscle fibers and to calculate the underlying Ca2+ release from the sarcoplasmic reticulum (SR) caused by the opening of Ca2+ permeable ion channels (RyRs). The recording of microdomain Ca2+ signals …
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TRANSCRIPTIONAL CONTROL OF Ca2+ SIGNALING IN T CELLS
… resulting in sustained elevation of cytosolic Ca2+ concentration critical for T cell activation. Sustained Ca2+ signals are important for the activation of Nuclear Factor of Activated T cells (NFAT), which is a key regulator of T cell activation through its transcriptional control of genes in …
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Angiotensin II signalling in sensory neurons
… on neuronal properties and function. Ca2+ imaging revealed that Ang II stimulated a rise in cytosolic Ca2+ in small-diameter sensory neurons which expressed the nociceptive markers TRPV1 and Nav1.8. This population of Ang II-sensitive neurons could be divided in two, with one …
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Mechanism and role of calcium in excitation-mucus secretion coupling in human colonic epithelium
… for developing new therapies. Cholinergic signals have been shown to stimulate mucus secretion, but the mechanism is not fully understood. The aim of this study was to investigate the molecular mechanism of calcium (Ca2+) coupling mucus (MUC2) secretion in colonic goblet cells in situ, …
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Suppression of Ca2+ Signaling Enhances Melanoma Progression
Ca2+ is a ubiquitous and dynamic second messenger molecule that is induced by many factors including receptor activation, environmental factors, and voltage, leading to pleiotropic effects on cell function including changes in migration, metabolism and transcription. As such, it is not surprising …
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Ca2+ signalling between the endoplasmic reticulum and lysosomes
Ca2+ is a universal and versatile intracellular messenger, regulating a vast array of biological processes due to variations in the frequency, amplitude, spatial and temporal dynamics of Ca2+ signals. Increases in cytosolic free Ca2+ concentration ([Ca2+]c) are due to influx from either an infinite …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
… acinar cell exocytosis requires precise calcium (Ca2+) signals. When cytosolic Ca2+ levels remain high, cellular functions are disrupted, which is associated with initiation of pancreatitis. Ca2+ signals are achieved through regulating endoplasmic reticulum (ER) stores, Ca2+ATPases, and …
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Modulation of GABAergic transmission in the cerebellar stellate cell network by neurotransmitter spillover and synaptic cross talk
… localized in the axon of stellate cells. Ca2+ imaging experiments performed with Two-photon Microscopy confirmed the role of presynaptic receptors in affecting the axonal Ca2+ signals that trigger GABA release. These findings support the relevance of presynaptic receptors and provide …
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Robust and Cross-Domain Anomaly Detection and Mitigation
… Semi-automated Analytical Tool for Astrocyte Ca2+ Signals and Vascular Responses in Neurovascular Coupling) to assist researchers in identifying anomalous Ca2+ signals and their corresponding vascular responses.
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A microfluidic approach for the initiation and investigation of surface-mediated signal transduction processes on a single-cell level
… beads led to a rapid rise of their cytosolic Ca2+ concentration which was analyzed by a dual-wavelength ratiometric fluorescence measurement of the Ca2+-sensitive dye Fura-2. After Ca2+ imaging, the cells were isolated individually from the microfluidic system and cultivated further. Cell …
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Endoplasmic Reticulum Morphoregulation Modulates Ca2+- Driven Brain Cell Functions
… of the ER is to store large amounts of calcium (Ca2+ ) and sequester the ion on low-affinity/high-capacity protein carriers. This enables the ER to rapidly release Ca2+ in response to specific signals, either globally or locally. This led me to investigate how the tubular ER structure supports …
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Complementation of an AtCAM3 knockout line restores wild type root growth and provides localization data through expression of a chimeric GFP:AtCAM3 fusion protein in Arabidopsis thaliana
… higher eukaryotes, and oscillations in cytosolic Ca2+ are one highly conserved mechanism for transducing these signals. In Arabidopsis thaliana there are four main isoforms of Calmodulin (CaM), one group of proteins that bind Ca2+ ions and interact with a multitude of protein targets to modulate …
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Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis
… to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific …
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Identification of CaMK-II Protein Targets in Tissue Culture and Zebrafish Embryos using Tandem Mass Spectrometry
<p>Calcium (Ca2+)/calmodulin-dependent kinase 2 (CaMK-II) is one member of a family of Ca2+/calmodulin-dependent protein kinases that responds to intracellular Ca2+ signals (Hudmon, A. and H. Schulman (2002)). CaMK-II is a multifunctional regulator of transcription, cell cycle progression, cell …
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The Role of Arabidopsis thaliana Cyclic Nucleotide-Gated Channels 2, 6, and 19 in Extracellular ATP Signalling
Calcium (Ca2+) is known as a secondary messenger in plant growth, development, and stress perception. Plants generate stress-specific Ca2+ signals (Ca2+ signatures) to translate extracellular signals into cellular responses. Extracellular Adenosine 5’-triphosphate (eATP) is a crucial signalling …
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Visualization of cellular signaling by fluorescence resonance energy transfer in response to biochemical and mechanical microenvironments
… Hence, visualization of the intracellular signals is a challenge of major importance for in-depth understanding of the relationship between biochemical/mechanical cues and various cellular responses. In this thesis, I have focused on Ca2+, cAMP-dependent protein kinas A (PKA), and focal …
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Stoichiometry of the interactions between endogenous Orai1 and STIM1 during store-operated Ca2+ entry
Ca2+ is a ubiquitous intracellular messenger. It regulates a variety of cellular activities, ranging from muscle contraction, neuronal transmission, secretion and cell growth to apoptosis. Store-operated Ca2+ entry (SOCE) is a major pathway of Ca2+ signalling and exists in almost all metazoans. …
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STIM Protein Coupling Domains and The Activation of Orai Channels
… are widely expressed endoplasmic reticulum (ER) Ca2+ sensor proteins able to translocate within the ER membrane to physically couple with and gate plasma membrane (PM) Orai Ca2+ channels. The coupling of STIM proteins and Orai Ca2+ entry channels generates “store-operated” Ca2+ signals crucial in …
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