Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Ca2+ signalling"”.
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Ca2+ signalling between the endoplasmic reticulum and lysosomes
Ca2+ is a universal and versatile intracellular messenger, regulating a vast array of biological processes due to variations in the frequency, amplitude, spatial and temporal dynamics of Ca2+ signals. Increases in cytosolic free Ca2+ concentration ([Ca2+]c) are due to influx from either an infinite …
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Towards an understanding of the role of Ca2+ signalling in neural stem cell activation
… Slo acts largely independent of the insulin signalling pathway, a canonical pathway required for NSC activation. Since Slo is a negative regulator of Ca2+ signalling, I investigated Ca2+ levels in quiescent and active NSCs. I have found that quiescent NSCs have significantly higher …
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BEYOND ARABIDOPSIS: UNRAVELLING THE HIDDEN WORLD OF CA2+ SIGNALLING ACROSS SPECIES VIA MULTISCALE IMAGING TECHNOLOGIES
… Among the various second messengers and signalling molecules, calcium ions (henceforth Ca2+) and glutamate (henceforth L-Glu) have recently reported to play a central role in long-distance signalling in Arabidopsis in response to biotic and abiotic stimuli. However, despite the power of …
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The role of extracellular matrix in modulation of myocyte Ca2+ signalling and contraction in the isolated rat heart
… observed that the amplitude of the intracellular Ca2+ transients that underlie contraction was reduced in isolated myocytes in comparison to Ca2+ transients from multicellular trabeculae. The role of ECM in myocyte Ca2+ modulation is poorly understood. We therefore hypothesised that Ca2+ handling …
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The role of intracellular Ca2+ in the arrhythmogenic activity of pulmonary vein cardiomyocytes
… mechanisms are unknown. Changes in intracellular Ca2+ signalling have been proposed to play an important role in the arrhythmogenic properties of the pulmonary vein. Therefore, the aim of this thesis was to study factors that might influence Ca2+ signalling in the cardiomyocytes. This involved …
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Inhibiting platelet scramblase activity
… activation, α-granule release and cytosolic Ca2+ signalling in response to a range of agonists. Ligand-based in silico methods were applied to identify new inhibitors of platelet scramblase activity. The pesticide thiodicarb was identified as a novel inhibitor of platelet scramblase activity …
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Investigating the role of TRPC3 in the biogenesis of extracellular vesicles
… characterised. Elevated intracellular calcium (Ca2+) induces EV release in a variety of cell types - with preliminary data to suggest the Ca2+ permeable transient receptor potential (TRP) channel TRPC3 may facilitate Ca2+-mediated EV biogenesis. TRPC3 contributes to the progression of ovarian …
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Elucidating TRPA1 Ion Channel Aberrations in Oesophageal Adenocarcinoma.
… OAC patients. TRPA1 is a ligand-gated, Calcium (Ca2+) -permeable membrane protein, which has recently been implicated in breast and prostate cancers. This doctoral thesis aims to uncover the tumour-promoting effects of TRPA1 in OAC, by using a multi-disciplinary approach including bioinformatics, …
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Regulation of Interferon Stimulated Genes Following Enveloped Virus Entry and Delivery of Viral Nucleic Acid
… membrane fusion and how this affects downstream signalling and upregulation of ISGs. We found that membrane perturbation by enveloped virus particles or p14 triggered cytosolic Ca2+ oscillations important for antiviral defence. Surprisingly, Ca2+ signalling seemed to act upstream of nucleic acid …
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Heterogeneity in the vascular endothelium enables parallel processing of multiple stimuli
… the mechanisms involved in endothelial Ca2+ signalling to muscarinic, purinergic and histaminergic activators and how the endothelium manages these extracellular signals when multiple agonists are present.;Using en face artery preparations we recorded the concurrent Ca2+ activity from …
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Stoichiometry of the interactions between endogenous Orai1 and STIM1 during store-operated Ca2+ entry
Ca2+ is a ubiquitous intracellular messenger. It regulates a variety of cellular activities, ranging from muscle contraction, neuronal transmission, secretion and cell growth to apoptosis. Store-operated Ca2+ entry (SOCE) is a major pathway of Ca2+ signalling and exists in almost all metazoans. …
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The physiological role of P2X4 receptors in lysosome function
… activated by ATP and with a high permeability to Ca2+. They are predominantly localised to lysosomes and from there can traffic to the cell surface. ATP levels within the lysosome are high but P2X4Rs are inhibited by the acidic pH. Previously, it was shown that the alkalinisation of lysosomes …
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Characterisation of Pharmacological Mechanisms and Potential Therapeutic uses of FK866
… finding that NAD+ plays a role in a variety of signalling pathways, including gene expression, Ca2+ signalling and DNA repair mechanisms, has sparked interest in the proteins involved in these pathways as potential pharmacological targets for drug development. Recently, FK866, a potent inhibitor …
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Exploring the role of Leucine Rich Repeat Kinase 2 within the innate immune system
… and can be phosphorylated by Myd88 directed TLR signalling. Functional experiments were performed using macrophages from WT and LRRK2 knockout mice. Many phenotypes and interactions have been described for LRRK2 in a neuronal or in vitro context; therefore experiments in macrophages were …
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Effects of Ca2+, microRNAs, and rosuvastatin on insulin-secreting beta cell function
… In this thesis I have investigated the role of Ca2+, miRNAs and rosuvastatin in the regulation of ion channels, exocytosis and insulin secretion of beta cells. For this purpose, pancreatic rat INS-1 832/13 beta cells, rodent animal models, and islets from human cadaver donors has been used. …
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Endoplasmic Reticulum Morphoregulation Modulates Ca2+- Driven Brain Cell Functions
… of the ER is to store large amounts of calcium (Ca2+ ) and sequester the ion on low-affinity/high-capacity protein carriers. This enables the ER to rapidly release Ca2+ in response to specific signals, either globally or locally. This led me to investigate how the tubular ER structure supports …
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Mitochondria-Endoplasmic Reticulum Contact Sites: Regulation and Roles in Coordinating Cell Fate Decisions
… reticulum (ER) contact sites (MERCs) form signalling platforms, which are required for cellular processes such as lipid metabolism, mitochondrial dynamics, mitochondrial Ca2+ signalling and apoptosis. MERCs are vital in coordinating cell fate decisions and their deregulation is associated …
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Endothelial NADPH oxidases as regulators of coronary microvascular dysfunction in diabetic cardiac remodeling
… expression, ROS/O2 - production, intracellular Ca2+ release (Flexstation) and cytokine release (cytokine array) in response to normal glucose (NG; 5.5mM) or high glucose (HG; 25mM). Experiments were repeated in coronary microvascular ECs (CMECs) to study differences in macro- vs. microvascular …