Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Ca2+ Sensor"”.
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Biochemistry and physiological role of otoferlin
… Es wird vermutet, es könne sich um den Ca2+-Sensor für die synaptische Vesikel Fusion an den inneren Haarzellen Band Synapse, und als Regulator für den Vesikel Nachschub handeln. Wir analysierten die C-terminale C2 Domäne C2F im Detail durch CD-Spektroskopie, Fluorimetrie und mit einem …
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Synaptic structure and function at the Drosophila larval neuromuscular junction : a molecular analysis of complexin and radish
… vesicle fusion is exquisitely regulated by Ca2+ such that neurotransmitter release can occur within 1 ms of an action potential reaching the presynaptic terminal. This feat implies the presence of both a Ca2+ sensor and a fusion clamp that prevents vesicles from fusing in the absence of …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
… well understood. This coupling is assumed to be Ca2+-dependent but the exact role of Ca2+ and its key effectors in the regulation of endocytosis are not clear. Using a genetically encoded pH-sensitive GFP tag expressed in cultured hippocampal neurons, I analyzed synaptic vesicle trafficking in …
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Elucidation of Jasmonate-Responsive Promoter Elements in the Calmodulin-Like Gene CML39 in Arabidopsis
… to biotic and abiotic stress. Calcium ions (Ca2+) have proven to be important components of signaling networks. Observations of stimulus-specific oscillations of cytosolic Ca2+ during signal transduction suggest that Ca2+ signals directly encode information. These stimulus-specific …
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Retrograde regulation of activity-dependent synaptic growth by Synaptotagmin 4
Ca2+ influx into pre- and post-synaptic compartments during neuronal activity is a key mediator of neurotransmitter release and synaptic plasticity. Although the role of presynaptic Ca2+ in triggering vesicle fusion is established, molecular mechanisms that underlie responses to postsynaptic Ca2+ …
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Molecular Basis of Coupling Calcium Sensing to Fast Membrane Fusion by Synaptotagmin-1 in Neurotransmitter Release
… the rapid release of neurotransmitters through Ca2+-triggered synaptic vesicle exocytosis. Synaptotagmin-1 (Syt1) acts as the Ca2+ sensor for fast, synchronous neurotransmitter release. Despite decades of research, the precise molecular mechanism of Syt1 action and the coupling of Ca2+ sensing …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… for fusion to occur. An increase in the local Ca2+ concentration at the synaptic terminal rapidly triggers neurotransmitter release. The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… for fusion to occur. An increase in the local Ca2+ concentration at the synaptic terminal rapidly triggers neurotransmitter release. The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… fast and strictly regulated by the intracellular Ca2+ concentration. To achieve this exquisite regulation, many proteins are involved. The central fusion machinery includes the SNARE proteins synaptobrevin, syntaxin and SNAP-25, as well as Munc18. Besides these universal components, many other …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… release machinery requires other factors such as Ca2+ sensor Synaptotagmin-1 and negatively charged lipids. Synaptotagmin interacts with the SNARE complex and the negatively charged lipids, and initiates the synchronous fast release by sensing the Ca2+ influx. It is crucial to investigate …
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Structural characterization of synaptotagmin I
… vesicle protein and is widely accepted as the Ca2+ sensor in fast synchronous neurotransmitter release. The protein is composed of one trans-membrane domain, an unstructured linker followed by two C2A domains identified as C2A and C2B. Each C2 domain is composed of an 8 stranded β-sandwich …
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STIM Protein Coupling Domains and The Activation of Orai Channels
… are widely expressed endoplasmic reticulum (ER) Ca2+ sensor proteins able to translocate within the ER membrane to physically couple with and gate plasma membrane (PM) Orai Ca2+ channels. The coupling of STIM proteins and Orai Ca2+ entry channels generates “store-operated” Ca2+ signals crucial in …
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Angiotensin II signalling in sensory neurons
… – and the presence of receptors for Ang II on sensory neurons may point to additional roles in nociception in inflammatory disease. Work in this Thesis sought to examine the identity of sensory neurons with which Ang II interacts, the mechanisms underpinning these interactions, and the …
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Reevaluating the functional role of the C₂A domain of synaptotagmin in neurotransmitter release
… Following depolarization of the nerve terminal, Ca2+ enters the presynaptic cell and drives fusion of vesicles with the membrane, releasing neurotransmitter. The synaptic vesicle protein, synaptotagmin, was identified as the Ca2+ sensor for this fast, synchronous neurotransmitter release. It is …
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Synaptic vesicle protein 2A-dependent function and dysfunction at the presynapse
… was investigated. SYT1 predominantly acts as the Ca2+ sensor for fast synchronous release at the presynapse, whilst the exact function of SV2A remains unknown to this day. In this study, the ablation of the AP-2 binding site in SV2A (Y46A) resulted in increased SYT1 surface expression and …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
Calcium (Ca2+) is an intracellular messenger regulating various physiological activities. The ‘store-operated calcium entry’ (SOCE) is a significant, almost ubiquitous Ca2+ signaling pathway and is triggered when intracellular Ca2+ stores (e.g., endoplasmic reticulum, ER) are depleted, either …
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Ca2+ signalling between the endoplasmic reticulum and lysosomes
Ca2+ is a universal and versatile intracellular messenger, regulating a vast array of biological processes due to variations in the frequency, amplitude, spatial and temporal dynamics of Ca2+ signals. Increases in cytosolic free Ca2+ concentration ([Ca2+]c) are due to influx from either an infinite …