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Showing 1 to 15 of 15 for “"Ca-ATPase"”.
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Thermodynamic effects of phospholamban on Ca-ATPase kinetics
The Ca-ATPase of sarcoplasmic reticulum removes cytosolic calcium to promote muscle relaxation. In the heart, the Ca-ATPase is regulated by phospholamban, which inhibits the Ca-ATPase by decreasing Ca-ATPase calcium sensitivity. However, the kinetic and thermodynamic mechanisms of inhibition are …
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Physical Mechanisms of Ca-ATPase Regulation in the Heart
The Ca-ATPase is an integral membrane enzyme which translocates two calcium ions from the cytoplasm of the cell to the sarcoplasmic reticulum lumen utilizing ATP breakdown as its energy source, in order to promote muscle relaxation. The focus of this research is the cardiac isoform of the Ca-ATPase …
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Photoaffinity labeling the nucleotide sites of the sarcoplasmic reticulum Ca²⁺-ATPase
… interaction with the sarcoplasmic reticulum Ca²⁺-ATPase. The TNP-8N₃-nucleotides were synthesized from ATP in three steps involving bromination in the 8-position of the adenine ring followed by displacement with an azido group and then trinitrophenylation of the resulting 8N₃-nucleotide with …
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Properties of the nucleotide binding sites of the Ca²⁺-ATPase of sarcoplasmic reticulum
Properties of the nucleotide binding site of the Ca²⁺-ATPase of skeletal muscle sarcoplasmic reticulum have been investigated. The study centred around interaction of the high affinity ATP analog, 2'-3'-0-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate, (TNP-ATP), with the Ca²⁺-ATPase. Defined …
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Properties of the non-catalytic nucleotide site of the Ca²⁺-ATPase of sarcoplasmic reticulum
… of the regulatory nucleotide binding site of the Ca²⁺-ATPase of skeletal muscle sarcoplasmic reticulum have been investigated. Previously, several lines of evidence have indicated the existence of both catalytic and regulatory nucleotide binding sites on the same polypeptide species. The present …
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Mechanism of sarcoplasmic reticulum Ca-ATPase and multidrug resistance P-glycoprotein probed by Pi-HOH oxygen exchange
Bibliography: leaves 135-146.
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Characterisation of the reaction of 1,4-phenylenebismaleimide with Ca²⁺-ATPase and elucidation of the intramolecular crosslink site
The SR Ca²⁺-ATPase is an ATP driven pump that removes calcium from the sarcoplasm and myofibrils to allow muscle relaxation. The sulfhydryl crosslinker, 1,4- phenylenebismaleimide, reacts with Ca²⁺-ATPase (110 kD) to form a species with an apparent molecular weight of 125 kD, as well as dimers and …
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The role of TNP-Nucleotides, LYS492 and CA²⁺chelators in the skeletal muscle sarcoplasmic reticulum CA²⁺atpase cycle
… the first part of this study, the kinetics of decay of TNP-nucleotide superfluorescence was investigated with a view to understanding the role of nucleotides and Lys492 in later steps in the catalytic cycle of the skeletal muscle Ca²⁺ATPase. It has been found previously, and verified here, that …
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Sarcoplasmic reticulum responses to repeated sprints, conditioning and dietary lecithin in the horse
… reticulum (SR) releases and sequesters calcium during muscle contraction and relaxation. Calcium uptake rates have been shown to respond to high intensity exercise, and we propose this response may be affected by conditioning and dietary lecithin. Ten Quarter Horses performed repeated …
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Magnesium Regulation of Calcium in Essential Hypertension
… is treated by palliative methods, since the causal factors are as yet unknown. A variety of both genetic and environmental factors probably combine to produce chronic high blood pressure. A genetic model of essential hypertension, spontaneously hypertensive rats (SHR), and its control strain …
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Mathematical modelling of energy demand and supply in the cardiac myocyte
… heart muscle are crucial for maintaining normal cardiac function, yet they are not very well understood. Although a number of mechanisms have been proffered by which mitochondrial supply of ATP can change to match varying workload in the myocardium, identifying the underlying regulatory pathways …
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The effects of fatigue on glycogen, glycogen phosphorylase, and calcium uptake associated with the sarcoplasmic reticulum of rat skeletal muscle
Skeletal muscle fatigue can be defined as the inability to produce a desired amount of force. Fatigue can not only limit athletic performance and rehabilitation, but it can affect one's ability to perform every day activity as well. Despite extensive investigation of muscle fatigue, little is known …
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Studies on intracellular calcium pools in a pancreatic cell line
… that inositol 1,4,5-trisphosphate (IP₃) can release Ca²⁺ from intracellular stores, the phosphatidylinositol signal transduction system has been recognized as a major transduction pathway in most cell types. However, IP₃ is not the only intracellular Ca²⁺ mediator. In some cells, …
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Intracellular calcium mobilization in cardiomyocytes of rats with experimental diabetes
… is well known that alterations in intracellular calcium, [Ca2]i, mobilization in the heart are among the major causes of cardiac dysfunction. To study the mechanism(s) involved in the development of diabetic cardio myopathy, [Ca2]i (determined by fluorescence microscopy and Ca-indicators) and …
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Age-associated changes to calcium handling proteins across the whole heart
Age is a major risk factor for the development of cardiac problems. The ageing process causes significant changes to cardiac structure and function. These are linked with the elderly heart’s diminished ability to respond to cardiac demand and associated with the increased frequency of myocardial …