Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"CYP2E1"”.
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Identification of Novel CYP2E1 Inhibitor to Investigate Cellular and Exosomal CYP2E1-Mediated Toxicity
<p>Cytochrome P450 2E1 (CYP2E1)-mediated hepatic and extra-hepatic toxicity is of significant clinical importance. Diallyl sulfide (DAS) has been shown to prevent xenobiotics such as alcohol- (ALC/ETH), acetaminophen- (APAP) induced toxicity and disease (e.g. HIV-1) pathogenesis. DAS imparts its …
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Proteomics of Oxidative Stress Using Inducible CYP2E1 Expressing HepG2 Cells and 3T3-L1 Adipocytes as Model Systems
… proteomes of 3T3-L1 adipocytes and an inducible CYP2E1 expressing HepG2 cells. Enhanced oxidative stress in hypertrophic adipocytes is associated with metabolic dysregulation and insulin resistance. Because mitochondria generate reactive oxygen species (ROS), we monitored changes to the adipocyte …
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The Role of Cytochrome P450 2E1 in Hepatic Ischemia Reperfusion Injury and Reactive Oxygen Species Formation
… after their degradation. Among P450 enzymes, CYP2E1 is unique because it is considered a leaky enzyme, producing significant ROS through uncoupling in the absence of substrates, accelerating its own degradation and reducing its half-life. In this dissertation, we generated and characterized a …
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Human Cytochrome P450 2E1: Functional Comparison to Cytochrome 2A13 and 2A6
… of the CYP2A6 and CYP2A13 enzymes versus CYP2E1. Because these enzymes metabolize both common, as well as unique, small molecule substrates, it is likely that only few key residue-substrate interactions are responsible for those metabolic capabilities that differ between them. Amino acid …
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Role of cytochrome P450 enzymes on alcohol/nicotine-mediated oxidative stress and cytotoxicity in monocytes/astrocytes: Implications for HIV-infected alcohol/tobacco users
… of abuse, such as tobacco. Cytochrome P450 2E1 (CYP2E1), which is induced by alcohol, is mainly involved in alcohol metabolism in the liver and extra-hepatic cells in chronic users. As a result of alcohol metabolism, CYP2E1 produces reactive oxygen species (ROS), which in turn inhibits …
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Vliv dihydromyricetinu a myricetinu na vybrané biotransformační enzymy
… myricetinu na aktivitu cytochromu P450 2E1 (CYP2E1) a N-acetyltransferas 1 a 2 (NAT1/2). Sledován byl jak vliv premedikace potkanů těmito flavonoidy na expresi a aktivitu těchto enzymů, tak i schopnost myricetinu a dihydromyricetinu inhibovat aktivitu CYP2E1 a NAT1/2. U CYP2E1 nebyla …
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The Effects of Ethanol and Nicotine on Hepatic and Brain CYP2 Family Enzymes in African Green Monkeys
… and nicotine on hepatic and/or brain levels of CYP2E1, CYP2B6 and CYP2A6 in African green monkeys. Monkeys were randomized into four groups (N = 10/group): an ethanol-only group, a nicotine-only group, an ethanol + nicotine group and a control (no drug) group. Ethanol (10% ethanol in sucrose …
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Effect of cell- and species-differences in the expression of drug metabolism enzymes on chemical-induced injury to the rat and human kidney
… expressed cytochrome P450 (CYP) 2B1/2, CYP2C11, CYP2E1, CYP4A2/3, and GSTα, but not CYP3A1/2, GSTµ, GSTπ, or GSTτ. rDT cells expressed CYP2B1/2, CYP2E1, and CYP4A2/3, GSTα, and GSTµ, but not CYP2C11, CYP3A1/2, GSTπ, or GSTτ. Expression of CYP2E1 was significantly higher in rDT cells than in rPT …
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Genetic polymorphisms in the drug metabolizing genes and their roles in the development of oesophageal cancer
… for the cytochrome P450 (CYP1A1, CYP1A2, CYP1B1, CYP2E1), sulphotransferase 1A1 (SULT1A1), glutathione S-transferases (GSTT1. GSTM1 and GSTP1) alcohol dehydrogenase (ADH2 and ADH3) and aldehyde dehydrogenase (ALDH2) because the products of these genes are involved in the metabolism or …
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Polimorfismos en genes metabolizadores del alcohol, acetaldehído y folato y riesgo de cáncer de pulmón
… implicados en el metabolismo del alcohol (ADH, CYP2E1), el acetaldehído (ALDH) y el folato (MTHFR) con el riesgo de cáncer de pulmón, así como la posible asociación entre el alcohol y el cáncer de pulmón, las interacciones gen-gen y gen-ambiente. Los polimorfismos en estos genes pueden dar lugar …
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HIV-1 gp120 and Methamphetamine-mediated Induction of Proinflammatory Cytokines/chemokines and Oxidative Stress in Astrocytes: Implications in Neuroinflammation
… The combination of gp120 and MA also induced CYP2E1 expression at both mRNA and protein levels, suggesting the involvement of CYP2E1 in ROS production. This was further confirmed by using selective inhibitors of ROS (Vitamin C), CYP2E1 (DAS), NOX, diphenyleneiodonium (DPI), and FWH reaction, …
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Genetically engineering a new system for the expression of cytochrome P450 enzymes in insect cells using novel P450 reductases
… CYPs, CYP2D6, CYP3A4, CYP1A1, CYP1B1, CYP1A2, CYP2E1 and CYP2C8 in conjunction with different CPR species (the native human CPR, variants of human CPR and the yeast CPR) using the baculovirus expression system. In my studies I have found that different CPRs have different coupling efficiencies …
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Toxicity and detoxification of imidacloprid in mouse ovarian follicles
… antral follicles. AOX1, AOX2, AOX3, CYP2D22, and CYP2E1 catalyze IMI reductions and CYP4F18 catalyzes IMI oxidations. IMI induced Cyp2e1 and Cyp4f18 in the follicles cultured for 96 hours compared to the controls. In Chapter 5, I localized nAChR subunit expression in the ovaries and I tested the …
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Investigating mechanisms of ovotoxicity of Perfluorooctanoic acid and Zearalenone exposure and the influence of altered metabolic status on ovarian function
… would be observed. The levels of ovarian EPHX1, CYP2E1 and GSTP1 proteins were evaluated by western blots of protein isolated from prepubertal pigs held in a thermal-neutral environment with ad libitum access to a standard swine diet, a thermal-neutral environment with a restricted access to a …
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Identifying unique cell states in early liver oncogenesis with transcription coupled repair
… data. I show that a subset of DEN-damaged Cyp2e1 expressing hepatocytes has the best expression fit with the mutational data, suggesting these may represent a subset of hepatocytes at the earliest stages of tumorigenesis. In conclusion, this work identifies a subset of DEN-damaged …
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Expression and activity of enzymes in the human placenta: pharmacological & toxicological consequences in AZT therapy
… were present and active in the human placenta. CYP2E1 protein was expressed but not active. CYPIA, CYP2EI and the UGT2B subfamily were expressed across gestation, but the UGTIA subfamily was only expressed in first trimester placentas. The localisation of XME (the syncytiotrophoblast layer …
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Farnesol-Mediated Regulation Of Hepatic Lipid Metabolism In Heparg Cells
… enzymes, such as CYP3A4, CYP2B6, CYP1A2 and CYP2E1 were also attenuated by co-treatment with farnesol. Our findings show for the first time that farnesol regulates human hepatic lipid metabolism and suppresses lipid accumulation in HepaRG cells under steatogenic conditions by upregulating …
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Effects of momordica charantia fruit juice on experimental diabetes and its complications
… diabetes an increased expression of CYP1AI, CYP2E1 and CYP4A2 proteins was also seen by western blot analysis. Mi charantia fruit juice feeding modulated the protein expression and catalytic activities in a tissue and isoenzyme specific manners. A marked decrease in hepatic glutathione (GSH)) …
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Investigation of experimental cell lines and non-invasive online sensor technologies in a 3D bioreactor system for extracorporeal liver support therapy
… showed the expression of hepatic markers (CYP2E1, albumin, CK18, CEBP-ß and MRP2) in B-13/H cells after hepatic trans-differentiation in the bioreactor system. In the second part of the study, the integration of multi-parametric sensors into the bioreactor system was investigated to allow …