Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"CYP1A"”.
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Hepatic CYP1A enzymes in fish as a biomarker of water pollution
… Induction of hepatic cytochrome P450 1A (CYP1A) enzymes in fish have been used as a biomarker to detect exposure to PAH and to a certain extent PCB-like compounds. In the present study, Chinook salmon (Oncorynchus tschawytscha) were used as an animal model (i) to examine the induction of …
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Response of CYP1A and VEGF in <i>Cyprinodon variegatus</i> When Exposed to Source/Dispersed Oil and Hypoxia
… <p>My results indicate that Cytochrome P450 1A (CYP1A) increases in a dose dependent manner when larval and adult <em>C. variegatus</em> are exposed to crude (WAF) and dispersed crude (CEWAF) oil. Vascular Endothelial Growth Factor (VEGF) expression increases in a dose dependent manner when …
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Involvement of Cytochrome P450 1A in the toxicity of aryl hydrocarbon receptor agonists : alteration arachidonic acid metabolism and production of reactive oxygen species
… hepatic microsomes, cytochrome P450s including CYP1A and CYP2B-like proteins catalyzed regioselective metabolism of AA to eicosatrienoic and hydroxyeicosatetraenoic acids. Benzo[a]pyrene (BP) treatment induced liver microsomal AA metabolism, but that effect varied with season. Endogenous AA …
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Involvement of cytochrome P450 1A in the toxicity of aryl hydrocarbon receptor agonists : alteration of arachidonic and acid metabolism and production of reactive oxygen species
… hepatic microsomes, cytochrome P450s including CYP1A and CYP2B-like proteins catalyzed regioselective metabolism of AA to eicosatrienoic and hydroxyeicosatetraenoic acids. Benzo[a]pyrene (BP) treatment induced liver microsomal AA metabolism, but that effect varied with season. Endogenous AA …
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Energetic costs of toxicant metabolism in rainbow trout ( Oncorhynchus mykiss)
… associated with increased detoxification. Two CYP1A inducers were employed, PCB-126 and BNF. Resveratrol, a documented inhibitor of CYP1A activity and gene expression, was used to correlate metabolic changes in CYP1A induction with any metabolic changes. PCB-126 and BNF effectively induced …
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Chemical mixtures and interactions with detoxification mechanisms and biomarker responses in fish
… for chemical interactions. Induction of the CYP1A isoform and the egg-yolk precursor vitellogenin in fish are two established biomarkers used to assess exposures to aromatic hydrocarbons and estrogens in the environment. This thesis focuses on regulation of these biomarkers, with emphasis on …
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Characterisation of mammalian cytochrome P450 enzymes by their metabolic activities.
… and MROD activities, and the concentration of CYP1A in the 16 human liver microsomal preparations. This suggests the involvement of CYP1A2 in the metabolism of harmine by human liver microsomes. Inhibition studies with selected anti-P450 antibodies and CYP-isoform selective inhibitors indicated …
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Broccoli bioactives: impact on enzyme induction, estrogen metabolism and human cancer cell growth
… An underlying mechanism may be upregulation of CYP1A and NQO1 activities, which may result in enhanced inactivation and subsequent excretion of potentially carcinogenic compounds. In this study, selenium enrichment resulted in increased levels of the indole glucosinolate neoglucobrassicin in the …
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Synthesis and Evaluation of Naturally Occuring Halogenated Bipyrroles in a Relevant Marine Species, Fundulus Heteroclitus
… were shown to induce the cytochrome P4501A (CYP1A) gene through activation of the AhR signaling pathway, similar to other anthropogenic pollutants. They also result in porphyrin accumulation in chicken embryo hepatocytes. Based on these studies, we have focused our efforts on examining the …
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Mechanisms of action for aryl hydrocarbon receptor ligands in the PLHC-1 cell line
… and significantly alter relative potencies of CYP1A induction, raising the possibility of artificial differences in measured potencies among cell types and laboratories. A quantitative pharmacological approach was used to show that both AHR binding affinity and intrinsic efficacy ofligands …
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Ricerca, mediante tecniche di proteomica, di biomarcatori proteici utili nella valutazione della qualità degli alimenti di origine animale
… levels of EROD supports the possible presence of CYP1A subfamily in the invertebrates, still today controversial, never identified previously in C. gallina and never isolated in the immune cells, as confirmed instead in this study with the identification of CYP1A-immunopositive protein …
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Chemical-sensitive genes in zebrafish (Danio rerio) early development - identification and characterisation of differential expression in embryos exposed to the model compound 3,4-dichloroaniline
… were involved in biotransformation pathways (cyp1a, ahr2), stress response (nrf2, maft, ho-1) and cell cycle control (fzr1). Differential expression upon 3,4-DCA exposure was detected below the LOEC (lowest observed effect concentration = 6.2 µM) of survival or developmental disorders of the …
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Breast Cancer Inhibition by Grape Constituents
… of both bioactivation enzyme, cytochrome P-450 (CYP1A), and detoxification enzymes such as glutathione S-transferase (GST) and quinone reductase (QR). These grape components also suppressed reactive oxygen species (ROS) formation. The constituents did not induce antioxidant response element …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… expressed ≥1.8-fold (p-values ≤5x10-4), and only CYP1A and CYP1B1 were induced >4- fold. Transcriptional responses to TCDD were highly dose-dependent, and adaptive responses were a prevalent feature of TCDD-modulated gene expression. Microarray analyses indicated induction of genes in three major …
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Characterization of P-glycoprotein expression as a multixenobiotic resistance mechanism in fish
… a P-gp substrate. We show that B[a]P and/or its CYP1A metabolites are not transported by P-gp in liver, brain, or ovary.
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Tetrachlorobiphenyl metabolism, toxicity, and regulation of cytochrome P50 expression in a marine teleost fish
… strong and sustained induction of hepatic CYP1A1 mRNA, protein content, and catalytic activity. A high TCB dose (5.0 mg/kg) elicited similar, strong induction of hepatic CYP1A1 mRNA, but not of CYP1A1 protein content or catalytic activity. This post-transcriptional "suppression" at the high …
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Estrogenic Activity of the Polybrominated Diphenyl Ether Flame Retardant Mixture DE-71
… knockout (ERαKO) mice. Liver cytochrome P450 1A (CYP1A) and CYP2B activities increased 2.5-fold and 7-fold respectively when DE-71 was administered PO, but only CYP2B increased (5-fold) after SQ treatment. Six OH-PBDE metabolites were found in mice after 34-day DE-71 treatment and all were able to …
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A study of gene expression in human normal and carcinogenic cell lines using qRT-PCR
… fact that transcription of CYPJAI was observed CYP1A1 activity was not detected even after cell treatment with CYP1A1 inducers. This was possibly due to scarcity of CYP1A! and limited level of haem in the extrahepatic tissue. This study demonstrated a novel basal and induced co-transcription of …
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Exploring the role and the function of Aryl Hydrocarbon Receptor (AhR) and Aryl Hydrocarbon Nuclear Translocator (ARNT) in T cells
… expressing Cre recombinase ‘knocked in’ to the CYP1A locus (CYP1A1Cre+/-) was backcrossed to the R26REYFP reporter mouse. In R26REYFP mice, a gene encoding EYFP is knocked into the ubiquitously expressed Rosa26 locus preceded by a loxP flanked stop sequence. CYP1A1 expression is controlled by …
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In search of human cytochrome P450 1A1 enzyme inhibitor
… family consists of three members; they are CYP1A1, CYP1A2 and CYP1B1. The mRNAs of CYP1A1 and CYP1B1 genes are known to be expressed in extra-hepatic tissues while that of the CYP1A2 gene is found in the liver amongst other tissues. Under normal circumstances, rarely has CYP1A1 protein …
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