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Showing 1 to 9 of 9 for “"CYP17A1"”.
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Mechanistic Studies of Two Steroidogenic Cytochromes P450, CYP17A1 and CYP21A2
Human CYP17A1 (P450c17 or 17alpha-hydroxylase/17,20-lyase) is a member of the P450 superfamily of proteins. This enzyme is responsible for hydroxylating the 17-position of pregnenolone or progesterone and cleaving the 17,20-carbon,carbon-bond of its hydroxylated products, which leads to the …
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Mechanistic investigation of human cytochromes P450 involved in hormone biosynthesis
CYP17A1 and CYP19A1 are the key cytochromes P450 involved in steroidogenesis. Mutations causing hypo- or hyper-activation of both these enzymes results in diseased states, including cancers. In addition to carrying out mono-oxygenation reactions, both CYP17A1 and CYP19A1 also catalyze carbon-carbon …
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Understanding the mechanism of androgen biosynthesis
Cytochrome P45017A1 (CYP17A1) is a multifunctional steroidogenic enzyme responsible for the 17-hydroxylation of pregnenolone and progesterone as well as the subsequent 17,20 carbon-carbon bond scission of its hydroxylated products in a reaction that constitutes the first committed step of androgen …
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Ruolo dei neurosteroidi nella modulazione dopaminergica
… in PPI, such as 17α-hydroxylase/C17,20 lyase (CYP17A1), 3α- and 3β-hydroxysteroid dehydrogenase (HSD), in Sprague-Dawley rats and we tested the drug like FIN in a reserpinized rat probability discounting task, a behavioral paradigm aimed at capturing critical behavioral aspect of gambling …
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Characterization of Microrna Expression Profiles and Role of Nodal-Related Genes in Zebrafish Ovarian Follicles
… primary cell cultures. The mRNA levels of cyp17a1, hsd3b2 and paqr8 were increased in response to Nodal treatment. Subsequently, we used CRISPR/Cas9 technology to generate ndr1 and ndr2 null mutants, which caused severe defects in early development. To overcome this lethality in vivo, we …
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The effects of green tea, green rooibos and their major flavonoids (EGCG and aspalathin) on testicular cell health in vitro
… namely Lhcgr, Star, Tspo, Cyp11a1, Cyp17a1 and Hsd17b3, as well as for Gapdh as a housekeeping gene. TM4 cells were also assayed for inhibin B secretion, lactate secretion and SCB integrity (by transepithelial electrical resistance). Within the tested concentration range of the teas …
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Toxicity of TCDD in the mouse antral follicle
… hours decreased transcript levels for Cyp11a1, Cyp17a1, Hsd17b1, and Cyp19a1, but increased Hsd3b1 transcript. TCDD exposure particularly lowered both Hsd17b1 transcript and HSD17B1 protein. However, TCDD exposure did not affect levels of E1 in the media. The down regulation of the AHR protein …
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Toxicity and detoxification of imidacloprid in mouse ovarian follicles
… of the steroidogenic regulators (Star, Cyp11a1, Cyp17a1, Cyp19a1, Hsd3b1, and Hsd17b1), estrogen receptors (Esr1 and Esr2), and apoptotic factors (Bax and Bcl2). I found that DNI, but not IMI, inhibited follicle growth and caused follicles to rupture in culture compared to control. IMI increased …
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The effects of adult exposure to genistein on female reproductive outcomes
… exposure decreased the expression of the enzyme Cyp17a1, the enzyme responsible for further metabolizing progesterone. Thus, explaining the observed increase in progesterone levels and decrease estradiol levels after exposure to genistein. Additionally, I hypothesized that preconception exposure …