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Showing 1 to 19 of 19 for “"CTnDOT"”.
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Excision of CTnDOT
Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2013-01-24T19:34:21Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Keeton_Carolyn.docx: 6779335 bytes, checksum: 32b917b01d2ce2946ce4c38114fe84a2 (MD5) Keeton_Carolyn.pdf: 4011034 bytes, …
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Characterization of IntDOT, the CTnDOT Integrase
… of the Bacteroides conjugative transposon CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a reaction that appears to differ in some …
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Excision of the Bacteroides Conjugative Transposon CTnDOT
In this study, we showed that expression of the exc operon is regulated by tetracycline and mediated by the regulatory proteins, RteA, RteB, and RteC. Tetracycline induces the expression of rteA and rteB, genes in the tetQ operon. Then, RteB induces the expression of rteC. RteC, subsequently, …
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Role of the core in CTnDOT integration
… by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can catalyze recombination between …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
CTnDOT is a conjugative transposon (CTn) that is found in many Bacteroides strains. Transfer of CTnDOT is stimulated 100--1000 fold if the cells are exposed to tetracycline (Tc). An operon that contains a Tc resistance gene, tetQ, and two regulatory genes, rteA and rteB, is essential for control of …
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DNA -Protein Interactions in the CTnDOT Excisive Intasome
The excision mechanism of CTnDOT is more complicated than any system studied to date. Excision requires multiple accessory proteins. Exc is one protein required for excision, however, its function is unknown. In an attempt to determine a role for Exc, the native wild type Exc protein was purified. …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
… of rteC. In order to determine whether CTnDOT regulatory genes can affect the expression of chromosomal genes in B. thetaiotaomicron , I used GeneChip and quantitative RT-PCR analyses. I showed that RteA and RteB are global regulators that induce the expression of various genes, in …
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Positive regulators of Bacteroides CTnDOT excision and transfer
… transfer of a Bacteroides Conjugative transposon CTnDOT. It was shown previously that a gene, rteC, is necessary for tetracycline-stimulated transcriptional regulation of the xis2c operon, which contains the excision genes. The protein encoded by this gene, RteC, did not have primary amino acid …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… recombination reactions that promote movement of CTnDOT that is an integrative conjugative element (ICE) found in Bacteroides spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence identity in the region where …
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
… transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. The CTnDOT Int protein catalyzes the in vitro integration reactions by a coupling sequence …
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Excision, transfer, and integration of the bacteroides integrative and conjugative element CTnDOT
… of the most well studied examples is the 65kb CTnDOT, which encodes both erythromycin and tetracycline resistances. Bacteroides are prevalent within the human gastrointestinal tract, which means that CTnDOT is capable of transferring to other resident microbes within this environment. The …
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Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT
… integrative conjugative element, or ICE) called CTnDOT, which is a 65 kb ICE that encodes resistance to the antibiotics erythromycin and tetracycline. A notable feature of CTnDOT is that excision from the donor chromosome and conjugative transfer are coordinately regulated upon exposure of donor …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
… harbor a conjugative transposon (CTn) called CTnDOT. CTnDOT carries two antibiotic resistance genes tetQ and ermF. A distinctive feature of CTnDOT is that both its excision and transfer are stimulated by tetracycline. The regulation of excision has been described previously. I provide here the …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… genes. One of these mobile genetic elements, CTnDOT, moves by conjugation and site-selective recombination. It is an example of a Conjugative Transposon (CTn). These elements were later classified as Integrated Conjugative Elements (ICEs). They frequently carry resistance to antibiotics and …
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Bacteroides Conjugative Transposons: Ecology and Mechanisms
CTnDOT is a 65 kb Bacteroides CTn that carries genes encoding for tetracycline resistance and erythromycin resistance. Previous studies had shown that the attDOT recombination site and integrase of CTnDOT (IntDOT) are sufficient and essential for integration in vivo. Even though the integrase of …
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Integration and Excision Mechanism of Bacteroides Conjugative Transposons
… is about the regulatory mechanism of RteB on CTnDOT. RteA and RteB are shown to be global regulators that regulate many genes on Bacteroides host. Whether RteB regulates CTnDOT and NBU1 excision directly or not is investigated in this study. Both RteB and the mutant are over-expressed in E. …
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Protein/DNA interactions during site-specific recombination
… (MTns). One such conjugative transposon is CTnDOT, which is 65 kb in size and encodes resistances to tetracycline and erythromycin. In recent years, CTnDOT has been implicated in the spread of antibiotic resistance among gut microbiota. The integration and excision of CTnDOT is mediated by …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
… proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA elements. The proteins isolated and characterized include BHP, a non-specific DNA-binding protein that is most likely involved in nucleoid organization, BHP2, a …
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The Role of Bacteroides Conjugative Transposons in the Spread of Antibiotic Resistance Genes, And, The Role of recA in DNA Damage Repair in Deincoccus Radiodurans
… of another Bacteroides conjugative transposon, CTnDOT, is presented. The second project involves identifying novel mechanisms of DNA damage resistance and DNA damage repair in the extremely radiation resistant organism Deinococcus radiodurans. Previously published studies had suggested that the …