Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"CTCF"”.
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A functional analysis of the epigenetic regulator CTCF
The highly conserved protein CTCF (CCCTC-binding factor) participates in many epigenetic regulatory functions, including insulation, imprinting, X chromosome inactivation, and both transcriptional activation and repression. Recent data has also suggested a prominent role for CTCF in mediating …
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The evolutionary genomics of CTCF binding and functional signatures in mouse.
… their involvement in the health and disease. The CTCF protein demarcates mammalian genomes into discrete transcriptionally active domains, providing the platform for complex spatial and temporal regulatory processing of genetic information that govern biological processes. In this thesis, I …
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The pathological and genomic impact of CTCF depletion in mammalian model systems
CCCTC-binding factor (CTCF) binds DNA, thereby helping to partition the mammalian genome into discrete structural and regulatory domains. In doing so, it insulates chromatin and fine-tunes gene activation, repression, and silencing. Complete removal of CTCF from mammalian cells causes catastrophic …
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Roles of CTCF and YY1 in T Cell Receptor Gene Rearrangement And T Cell Development
… have previously shown that CCCTC-binding factor (CTCF) regulates Tcra gene transcription and rearrangement through organizing chromatin looping between CTCF- binding elements (CBEs). This study is one of many showing that CTCF functions as a chromatin organizer and transcriptional regulator …
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The Functional Role for Chromatin Loops in Gene Expression Control During Human Neuron Maturation
… causal link among higher-order chromatin loops, CTCF occupancy, and mRNA levels in post-mitotic human neurons remains an open question. Here, we summarize the field of epigenetics and chromatin in neuroscience, examine neural model systems, and build an auxin-inducible degron to deplete the …
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The chromatin landscape of colorectal cancer cells
… by chromatin insulating proteins such as CTCF (CCCTC-binding factor) and multiprotein complex, cohesin. The dysfunction of which, through mutational and epigenetic aberrations, directly impacts a plethora of chromatin contacts and the resultant transcriptional profiles within each cell. …
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Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… previously. Chromatin architectural protein Ctcf has previously been implicated in the control of long-range enhancer-promoter contacts and inter-chromosomal interactions. Ctcf is broadly expressed in the skin including epidermal keratinocytes and hair follicles. Conditional Keratin 14-driven …
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3D chromosome structure and chromatin proteomics
… structures. Enhancer (H3K27ac) and insulator (CTCF) locations were mapped using ChIP-sequencing, whereas 3D chromosome structures were detected by cohesin-ChIA-PET. 3D chromosome structures connecting insulators (CTCF-CTCF loops) were shown to form topologically associating domains (TADs) and …
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The epigenetics and role of Dopa Decarboxylase in heart development
… occurs from both parentally inherited alleles. CTCF plays a central role in controlling gene expression by regulating chromatin organisation, and has been shown to be fundamental for imprinting of the Igf2/H19 locus through its binding to the germline differentially methylated region (gDMR). …
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Mechanisms of epiallelic variation in mouse
… role of the multifunctional DNA binding protein CTCF at VM-IAPs. CTCF is enriched at VM-IAPs but not at IAPs in general. I postulate that CTCF antagonises the DNA methylation machinery during early development, thus driving variable methylation. I use CRISPR/Cas9 mutagenesis in mouse embryonic …
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Regulatory variation and human disease
… patterns of a major sequence-specific regulator (CTCF) in relation to genetic variability in binding site sequences across a three-generation pedigree as well as 19 diverse human cell types. We identified hundreds of genetic variants with reproducible quantitative effects on CTCF occupancy (both …
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Changes of nucleosome positioning and 3D chromatin organization in cell transitions
… domain boundaries is the architectural protein CTCF that binds to thousands of locations in the genome and changes the chromatin configuration during cell differentiation or cancer development. Chapter 1 of this thesis provides an overview of the field of chromatin folding and a premise of the …
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Regulatory architecture of the Pax6 locus
… Work in other loci suggests a key role for CTCF and cohesin (subunit Rad21) in chromatin organisation and long distance regulatory interactions. ChIP-chip for CTCF/Rad21 across the Pax6 locus identified numerous sites within the gene and at distal regulatory locations. The majority of these …
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Predicting genomic interactions using deep learning
Physical promoter-enhancer and CTCF-CTCF interactions organize the human genome in 3-dimensions, and contribute to the regulation of gene expression. Hi-C and related approaches have enabled profiling of these interactions, though how the instructions for these interactions are encoded in the …
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Understanding the epigenome using system genetics
… several assays are performed: FAIRE-chip, CTCF- seq, RNA-seq and DNA-seq. In the first part, the discovery and characteristics of FAIRE-QTLs is presented. The identified FAIRE-QTLs show strong overlap with other molecular QTLs, histone modifications, and transcription factors. The second …
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Therapeutic testing and epigenetic characterization of Friedreich Ataxia
… for the presence of the CCCTC-binding factor (CTCF) was also performed on FRDA patient cerebellum samples. Overall, lower levels of CTCF were detected in FRDA-associated FXN alleles, suggesting the potential involvement of CTCF in the regulation of FXN transcription.
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Insights into transcriptional regulation from natural and induced variation in closely related species
… I explore the evolutionary dynamics of the CTCF (CCCTC-binding factor) binding among mouse species and its interplay with the establishment and maintenance of topologically associating domains (TADs). TADs make up a level of the 3D genome organisation that dictates formation of regulatory …
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Investigating and exploiting the latency-associated expression of the human cytomegalovirus gene US28 in early myeloid lineage cells.
… THP-1 monocyte-like cell line. This identified CTCF, a transcription factor which appears to be modified by US28 in THP-1 cells. I showed that CTCF has a repressive effect on the HCMV MIEP, and that CTCF likely plays a role in HCMV latency. In summary, this work provides insights into the role …
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