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Showing 1 to 20 of 32 for “"CRM1"”.
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Untersuchung des CRM1-abhängigen RNA-Exports mittels Repräsentativer Differenz-Analyse
… Substrate anhand bestimmter Transportmotive. CRM1 ist ein Export-Rezeptor, der seine Substrate über Leucin-reiche Kern-Export-Signale bindet und daraufhin exportiert. Dieser Transport-Rezeptor hat ein weites Spektrum an Export-Substraten, da es sowohl Proteine als auch Protein-RNA-Komplexe wie …
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Nuclear Export Receptor CRM1 Recognizes Nuclear Export Signals with Diverse Conformations
The Chromosome Region of Maintenance 1 or CRM1 protein facilitates export of hundreds of proteins and RNA molecules from eukaryotic cell nuclei. CRM1 recognizes its protein cargoes by their 8-15 residues-long nuclear export signals or NESs, which bind to a hydrophobic groove in CRM1. NESs are …
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Die Funktion von NLP1 im CRM1-abhängigen Protein-Export aus dem Zellkern
… erfolgt unter Beteiligung des Exportfaktors CRM1. Dieser erkennt einen hydrophoben Bereich des zu exportierenden Proteins (NES = nuclear export signal), bindet ihn in Gegenwart von RanGTP und vermittelt anschließend den Durchtritt durch die Kernpore. Die meisten NES-Substrate besitzen eine …
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Nuclear Export Signal Recognition by CRM1 Carrying the Oncogenic E571K Mutation and Structure-Based NES Prediction
… processes in the nucleus and the cytoplasm. CRM1 is the major nuclear export mediator, which facilitates nuclear export of hundreds of protein and RNA molecules. CRM1 is essential to the survival of eukaryotic cells and is overexpressed or mutated in a variety of cancers. This involvement of …
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Expression and regulation of the nuclear transport proteins, Crm1 and Kpnß1, in cervical cancer and transformed cells
Includes abstract. Includes bibliographical references (leaves 204-223).
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Structural and functional analysis of exportin-cargo recognition
… der Sox-Familie importiert.CRM1 (auch Exportin 1 genannt) ist das mit Abstand vielseitigste Exportin der Zelle: CRM1 erkennt eine enorme Vielzahl strukturell verschiedener Fracht-Proteine ( Kargos ). Wie diese bemerkenswerte Multispezifität bewerkstelligt wird und wie Ran die …
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Die Rolle von eIF-5A und Kernaktin bei Kernexportprozessen
… wird. Neben dem generellen Exportrezeptor CRM1 konnte auch der eukaryotische Initiationsfaktore 5A (eIF-5A) als ein Bindungspartner von Rev identifiziert werden. In dieser Arbeit konnte nun gezeigt werden, daß eIF-5A ein essentieller Faktor für den Rev-vermittelten RNA Export ist. …
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Implications of a CALM-derived Nuclear Export Signal for CALM-AF10-mediated Leukemogenesis
… is recognized by the nuclear export receptor, CRM1. Classically, CRM1 binds to NES-containing proteins and mediates their export from the nucleus to the cytoplasm through the nuclear pore complex. Through structure-function analyses, we determine that the CALM-derived NES is necessary and …
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Investigating nuclear transport proteins as secreted cancer biomarkers
… protein family; Kpnβ1, IPO5, IPO7, TNPO1, CRM1, CAS, Kpnα2 and Ran in a normal epithelial cell line (hTERT-RPE1) in comparison to transformed (SVWI38 and CT-1), cervical cancer (HeLa and CaSki) and oesophageal cancer (WHCO5 and KYSE 30) cell lines using Western blot analysis. Our data …
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Molekularbiologische und bioinformatische Analyse des Proteins KIAA0592/RISP und des dazugehörigen Gens
… gezeigt, dass Risp Interaktionen mit Rev und Crm1 eingeht und in der Lage ist, zumindest zu dimerisieren. Für Rev ist bekannt, dass es ebenfalls mit dem Kernexportrezeptor Crm1 interagiert, und dass diese Eigenschaften essentiell für die Aktivierungsfunktion von Rev ist. Deswegen wurden die …
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Nuclear accumulation of G-actin in cellular senescence: Reduced expressions of exportin 6 and Ran small G protein
… exclusively kept in the cytoplasm by exportin 1 (CRM1)-driven nuclear export. However, CRM1 has been reported to be not directly involved in actin export from vertebrate or Drosophila nuclei. On the other hand, exportin 6 (exp6) has been found as a novel family member from higher eukaryotes. Exp6 …
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The role of human and Drosophila NXF proteins in nuclear mRNA export
… in mRNA export in Drosophila Schneider cells. Crm1 is a transport receptor implicated in the export of a variety of non-mRNA and protein cargoes. In addition, human Crm1 has been suggested to be involved in the export of a specific mRNA species, serving as a "specialized" mRNA export receptor. …
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Mechanisms of Human LARP6 Nuclear Export
… export in Chromosome Region Maintenance 1(CRM1) in vivo, we performed inhibition studies in cell lines that endogenously expressed LARP6. Second, we developed two related approaches that leveraged the recombinant LARP6 protein approaches that had been established in the lab. To determine …
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Understanding the role of long non-coding RNAs in neuroblastoma development and progression
… Higher expression of the p53 exporter CRM1 in NBAT1 depleted cells contributes to p53 cytoplasmic localization, while CRM1 inhibition in these cells restores p53 localization. We observed that combined inhibition of CRM1 and MDM2 sensitized aggressive NB cells with cytoplasmic p53, …
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Characterizing the RanGAP1-RanBP2 complex in mitosis
… zur Identifikation des Kernexportrezeptors Crm1 und der GTPase Ran als stabile Komponenten in einem Komplex mit RanGAP1, RanBP2 und Ubc9 in mitotischen Zellen. Zusätzlich schien dieser Komplex zahlreiche weitere Proteine in substöchiometrischen Mengen zu enthalten. Diese könnten …
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Identification and characterization of Risp
… drug which has been shown to specifically block Crm1 (exportin) mediated export, resulted in nuclear accumulation of Risp-GFP, showing that the nuclear export of Risp, like that of Rev, is Crm1-dependent. Using bioinformatic tools able to detect weak homologies with high specificity, sequence …
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Identification of hepatitis C virus core protein residues critical for the interaction with the cellular DEAD-Box Helicase DDX3 and their functional relevance
… shuttling protein, utilising the CRM1 export pathway. To confirm this, DDX3 localisation was analysed in the presence of the CRM1 inhibitor leptomycin B (LMB). In the absence of LMB, DDX3 was seen to have a diffuse cytoplasmic localisation while a small proportion was also seen in …
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Mechanistic and Physiological Studies of the Insulin-Dependent Regulation of FOXA2
… signal and is excluded from the nucleus via a CRM1- dependent pathway in response to insulin signaling. Our data provide direct evidence that nuclear export-defective Foxa2 is phosphorylated and inactivated by insulin both in vitro and in vivo, suggesting that phosphorylation itself is the main …
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Identification of Oncogenic KRAS-Associated Vulnerabilities in Non-Small Cell Lung Cancer
… of a key nuclear export receptor, XPO1 (a.k.a. CRM1), was sufficient to induce robust and selective apoptosis in KRAS mutant NSCLC cells in vitro and to cause significant impairment of KRAS mutant tumor growth in vivo. Mechanistically, XPO1-depedent nuclear export machinery was required to …
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LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF POST-TRANSLATIONAL MODIFICATIONS TO HMGB1 AND ITS REGULATED SECRETION IN LUNG CANCER
… (XPO1) also known as chromosomal maintenance 1 (CRM1) rather than acetylation or oxidation. Malignant mesothelioma (MM) cell lines were found to constitutively secrete unacetylated HMGB1, and the cysteine oxidation profiles in HMGB1 from cisplatin-mediated, dimethyl sulfoxide-mediated, and MM …
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