Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"CRISPRa"”.
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CRISPR activation screening at single-cell transcriptomic resolution: discovering new regulators of zygotic genome activation
… method that combines pooled CRISPR activation (CRISPRa) with single-cell transcriptomics and apply it to discover new epigenetic and transcriptional regulators of ZGA in mouse embryos. In chapter 1, I introduce the biological context and latest technological advances related to CRISPR and …
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Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
… on this foundation, we engineered interconnected CRISPRa/i circuits capable of executing dynamic genetic programs in both cell-free and bacterial systems, showing that these circuits can be layered to achieve temporal regulation and logic-based behaviors. To increase circuit complexity, we …
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Methods for CRISPR Cas12a Multiplexing in Mammalian Systems
… editing activities, including gene activation (CRISPRa). However, only a small number of genes can currently be targeted at one time, due to a limit on the number of gRNAs that can be successfully placed into a guide array. This is primarily due to the difficulty of synthesizing arrays with a …
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CRISPR-based functional genomics to study gene regulatory architecture and consequences of genetic variation
… multiplex, single-cell CRISPR activation (CRISPRa) screening, a method I developed during my PhD and utilized to identify both proximal and distal cell type-specific regulatory elements that are capable of increasing target gene expression when activated via a specific CRISPRa perturbation. …
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From iPSCs to Engineered Heart and Liver Models: Modulating Genetic and Extracellular State for Maturity
… factors. Functional interrogation revealed that CRISPRa-mediated activation of early endoderm TFs enhanced hepatic function. siRNA knockdown of AP-1 family members, FOS, JUN, FOSL1, FOSL2 changed albumin secretion. Additional analyses showed persistent activation of EMT and TGF-β signaling in …
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PRC2 GATEKEEPS THE BALANCE BETWEEN DIRECT AND INDIRECT NEUROGENESIS AND CONTROLS NEURONAL MIGRATION DURING HUMAN CORTICOGENESIS
… subsequent neuronal migration, we developed a CRISPRa-based system to upregulate AJAP1 in control CBOs. This approach allowed us to isolate the molecular and cellular effects of AJAP1 dysregulation in WVS and demonstrate its regulatory relationship with EZH2 in neuronal migration. This study …
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TET1 AND TRANSCRIPTION FACTOR REGULATION OF LINEAGE SPECIFICATION DURING MOUSE GASTRULATION AND EARLY ORGANOGENESIS
… Chapter 5, I utilise a pooled CRISPR activation (CRISPRa) spontaneous differentiation experiment with single cell transcriptome readout to test 146 TFs with putative cell type-specific activities, based on analysis of a paired single cell transcriptome and accessibility atlas from mouse …
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Functional genetics reveals surprises about the mechanisms of action of chemotherapeutics
… These new technologies, known as CRISPRi and CRISPRa, were demonstrated to easily and efficiently facilitate screening of genes influencing tumor growth and treatment relapse. Thus, future utilization of these tools should permit faster discovery and translation of new findings to the clinic.
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Engineering programmable nucleases for in vivo and in vitro applications
… tools, such as base editors, prime editors, and CRISPRa/i systems, to achieve enhanced functionalities and expanded applications.