Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"CRISPR-Cas9 system"”.
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Genetic screens in vivo using the CRISPR/Cas9 system
An unmet and paramount need in the field of cancer research is to rapidly translate basic biological findings to clinically relevant therapeutics for cancer patients. Recent technological advances have generated many innovative applications to cancer biology and in a short time have yielded a …
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The direct injection of CRISPR/Cas9 system into porcine zygotes for genetically modified pig production
… examine the efficacy of direct injection of the CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/ CRISPR associated protein 9) in pigs and to overcome shortcomings that resulted after direct injection into the cytoplasm of developing zygotes. By using direct injection …
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CRISPR-mediated interrogation of small cell lung cancer
… and metastasis. The development of the CRISPR-Cas9 system has greatly facilitated genome editing in mammalian cells, leading to its widespread adoption for various applications in cancer biology. We have utilized this system in two complementary ways to investigate the molecular …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… along with fast neutron mutagenesis, a dual gRNA CRISPR/Cas9 genome editing system was developed as a molecular genetic tool for gene discovery and crop improvement in soybean. In order to demonstrate the efficiency and ability to simultaneous by edit homeologous genes using a CRISPR/Cas9 system …
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Modulation of DNA repair pathway after CRISPR/Cas9 mediated Double Stranded Break
The CRISPR/Cas9 system has become the predominant tool for genome editing. Targeted modifications can be introduced while repairing double strand breaks (DSBs), induced by the CRISPR/Cas9 system. The DSB is repaired by either non-homologous end joining (NHEJ) or homologous recombination (HR), and …
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Regulation of Intestinal Epithelial Thymic Stromal Lymphopoietin Gene Expression by Retinoic Acid Receptor Alpha
… mice). We sought to generate an experimental system to identify signaling pathway(s) or mechanism(s) that might be governing these effects, specifically RA signaling, as this data suggested there might be contributing factors intrinsic to IECs. We chose to establish a knockout cell line using …
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Optimizing CRISPR/Cas9 Gene Targeting to Investigate Cone Photoreceptor Development in the Chick Retina
… this question, this paper studies the effect of CRISPR/Cas9-mediated mutagenesis of two key transcription factors, Otx2 and Nr2e3, on retinal progenitor cells and cone photoreceptors throughout the development of the retina. This study also explores optimization of the CRISPR/Cas9 system to …
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Development of an inducible transcriptional control system in plasmodium falciparum with applications to targeted genome editing
… to reconstitute the T7 RNA polymerase expression system in P. falciparum, functionally creating the first system for directed expression of non-coding RNA in the parasite. We were then able to use this expression system to adapt a clustered regularly interspaced short palindromic repeats …
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Characterization of synaptotagmin 7 function in neurotransmission and its subcellular localization at synapses
… role as an asynchronous Ca²⁺ sensor, we used the CRISPR-Cas9 system to generate mutations in the Syt7 locus and introduced tags to label the endogenous protein in Drosophila. Electrophysiology, FM1-43 analysis and quantal imaging revealed that release probability is elevated 2-fold at larval …
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CRISPR/CAS9 mediated gene editing for the improvement of beef and dairy cattle
… In this study, we examine how we can use the CRISPR/Cas9 system to induce single-base pair substitutions, thereby improving animal genetics through removal of undesirable or insertion of beneficial mutations. In Angus beef cattle (Bos taurus), a mutation in the NHL-repeat containing 2 gene …
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DESIGN AND INVESTIGATION OF DUMBBELL-SHAPED DNA MINIMAL VECTORS FOR CRISPR/CAS9 EDITING
… DNA vectors for the delivery of the CRISPR/Cas9 system. Using three targets, i.e. glucose-6-phosphate dehydrogenase (G6PD) Mahidol, G6PD Mediterranean and Southeast Asian Ovalocytosis, and a dual reporter assay, we demonstrated that dumbbell vectors offer a viable alternative to …
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Development and Characterization of Viral-Based Gene Editing In Vivo
… Viral (AAV) vectors packaging the CRISPR/Cas9 system (AAVCRISPR) can efficiently modify disease-relevant genes in somatic tissues with high efficiency. AAV vectors are a preferred delivery vehicle for tissue-directed gene therapy because of their ability to achieve sustained …
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Regulation and Function of the Dscam1 Extended 3' UTR mRNA
… extended 3' UTR mutant using the relatively new CRISPR/CAS9 system. We show that the Dscam1 extended 3' UTR loss of function mutants have severe deficiencies in locomotor activity and display an increased mortality rate. We have identified that in S2 cells the RNA binding protein ELAV promotes …
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ANALYZING THE POTENTIAL IMPACT AND ETHICAL QUESTIONS SURROUNDING CRISPR-CAS9 IN EMBRYONIC GENOME EDITING
… Impact and Ethical Questions Concerning CRISPR-Cas9 in Genome Editing covers the current state of genome editing, and more specifically, the CRISPR-Cas9 system. The goal of this thesis is to answer the broad questions; “What is genome editing?,” “Who can afford it?,” and “What are the …
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Zinc Finger Protein 36L1 (ZFP36L1): Gene Expression, Regulation and Interactions with Immune Receptors in Human Tumour Cells.
… and MC38. To study the function of ZFP36L1 a CRISPR-Cas9 system was used to generate ZFP36L1 knockout MC38 cell line. Our results show that ZFP36L1 limits production of TNF α and VEGF A. Our preliminary data also show co-localization of ZFP36L1 with CD44 upon cellular permeablization, which …
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Investigating the role of microglia in myelin development
… are resident immune cells in the central nervous system (CNS) and carry out important functions not only in injury and disease but also in homeostatic conditions. While the role of microglia in myelination has been explored by previous studies, little is known about the precise mechanism. …
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Using CRISPR/Cas9 to modify the genome of cattle
… production. The gene editing technology CRISPR has expanded the possibilities of gene editing. The Cas9 nuclease creates a double strand break which can be repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR). The goal of this experiment was to create a single …
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Investigating The Role of Prmt1 and Arginine Methylation of Hsp70 In Human Pancreatic Cancer
… By using HSP70 knockout cells generated by CRISPR/Cas9 system and a series of wildtype and methylation sites mutant HSP70 expression vectors, I demonstrated that PRMT1-mediated arginine methylation was essential for the drug resistance function of HSP70 in pancreatic cancer cells.</p> …
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